Determination of mesenchymal stem cell; Roles of BMP4 and Scara5

Abstract

The mesenchymal cell is a multipotent stem cell with the capacity to give rise to multiple cell types.Recently, a number of studies have demonstrated a role of BMP4 in the commitment of mesenchymal stem cells (C3H10T1/2) into the adipocyte lineage. Previously we investigated the genetic events underlying adipocyte commitment, for which C3H10T1/2 stem cells were treated with 5‐azaC and selected preadipocyte clonal lines, named A33 line. In this study, we analyzed the BMP4 promoter region in 10T1/2 and A33 cell. The activity was decreased dramatically to the level measured for the constructs between −197 and −60 bp promoter sequence. And then, we searched that global changes in gene expression of 10T1/2 and A33 cells were examined by microarray. We found that gene expression of scavenger receptor class A member 5 was significantly increased A33 compared 10T1/2. Further studies are underway to identify a transcription factor bound to this region, which will reveal the BMP4 upstream pathway essential for the adipocyte commitment of stem cells. And we will investigate the role of scara5 in mesenchymal stem cell development.This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2011‐0030711 and 2011‐0015665) and by Korean Research WCU grant (R31‐2008‐000‐10086‐0) from the Korean Ministry of Education, Science, and Technology.