Determination of mecoprop in river- and ground-water samples by HPLC using a fluorescence detector.

Tanaka, Akio; Mikuriya, Ryozo; Matumoto, Ryuji; Yamazaki, Yoshinari; Morita, Hisao

doi:10.2116/bunsekikagaku.42.6_357

Cited by 1 publication

(5 citation statements)

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“…Mecoprop has been found frequently in UK surface waters at concentrations of between 0·5 and 11 μg l −1 (Croll 1991). The limit of detection for the bacterial scAb is similar to that obtained using HPLC equipped with a fluorescence detector (Tanaka et al . 1993).…”

Section: Discussionsupporting

confidence: 77%

“…Current approaches for the detection of mecoprop are technically demanding, time‐consuming and very expensive, using methods such as gas chromatography/mass spectrometry and HPLC (Tanaka et al . 1993; SanchezRasero et al .…”

Section: Discussionmentioning

confidence: 99%

“…The increased expression yield obtained through the Skp system appears to be significantly increasing the rate of spontaneous dimerization of our product. Current approaches for the detection of mecoprop are technically demanding, time-consuming and very expensive, using methods such as gas chromatography/mass spectrometry and HPLC (Tanaka et al 1993;SanchezRasero et al 1998;Tsipi et al 1998). In contrast, immunoassays could allow quick and inexpensive analysis of phenoxyacids in the laboratory or in the field.…”

Section: Discussionmentioning

confidence: 99%

“…1) have been widely used in the last 30 years to control broad-leaved weeds and other vegetation. The mass manufacture and extensive use of this pesticide have produced considerable pollution in the environment, especially in groundwater, rivers and streams (Croll 1991;Tanaka et al 1993). The toxicological significance of pesticide residues remains uncertain in many instances, but ingestion of mecoprop by humans has been found to cause pyrexia, nausea, hypotonia, confusion, coma, metabolic acidosis, convulsions, cytoskeletal perturbation and renal damage.…”

Section: Introductionmentioning

confidence: 99%

“…1) have been widely used in the last 30 years to control broad‐leaved weeds and other vegetation. The mass manufacture and extensive use of this pesticide have produced considerable pollution in the environment, especially in groundwater, rivers and streams (Croll 1991; Tanaka et al . 1993).…”

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confidence: 99%

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Reduced toxicity of expression, in Escherichia coli, of antipollutant antibody fragments and their use as sensitive diagnostic molecules

Strachan

Williams

Moyle³

et al. 1999

J Appl Microbiol

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Single-chain antibody fragments (scAb), specific for the chlorophenoxy acid herbicide mecoprop, have been expressed and purified from the bacterium Escherichia coli. Co-expression with the colE1-compatible, arabinose-inducible, skp expression vector pHELP1 prevented bacterial lysis and significantly increased both total and functional expression yield. The periplasmic protein, SKP, may have a role as a generic detoxification protein. Surface plasmon resonance (BIAcore 2000) analysis confirmed that the purified scAb retained similar binding kinetics to the monoclonal antibody (Mab) from which it was cloned. In competition ELISA, the bacterial scAb showed the same specificity for mecoprop and a related herbicide, MCPA, as the Mab but an increase in sensitivity for free antigen in all ELISA formats. Bacterially expressed antibody fragments provide a simple, sensitive and cost-effective alternative to the traditional production of diagnostic Mabs via tissue culture.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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