Determination of mean whole body entracellular pH in unanesthetized dogs

Albers, C.; Saborowski, F; Usinger, W.; Scholand, Ch.

doi:10.1016/0034-5687(75)90071-7

Cited by 7 publications

(2 citation statements)

References 7 publications

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“…In their discussion of the errors involved in the determination of pHi, Albers et al (2,8) stress the importance of the accuracy of the determination of the extracellular water volume and the total amount of dimethadione in the body, and to a lesser degree that of the determination of the total body water volume.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous Determination of Dimethadione and Trimethadione by Infrared-Spectrometry: Application for Mean Intracellular pH Measurement

Zweens¹,

Frankena²

1984

Clinical Chemistry and Laboratory Medicine

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A simple infrared-spectrometric method for the Simultaneous determination of dimethadione and trimethadione in plasma is described. The method is based on the absorption band of the carbonyl group for trimethadione at the wavenumber of 1740 cm" 1 and for dimethadione at the wavenumber of 1770 cirT 1 . The accuracy and precison of the method are excellent; at a dimethadione and trimethadione concentration of 0.5 mmol • 1 the coefficient of Variation for the determination of both compounds is <1%, which is better than that for the ultraviolet spectrometric or gas-liquid Chromatographie methods. The method can be used for the determination of the ratio trimethadione/dimethadione in the clinical setting äs a sensitive check of the patient's adherence to therapy. It can also be used for the determination of dimethadione äs an indicator for the mean body intracellular pH. Simultane Bestimmung von Di-und Trimethadion durch Infrarot-Spektrometrie: Anwendung zur Bestimmung des mittleren intrazellulären pHZusammenfassung: Eine einfache Methode zur simultanen infrarotspektrometrischen Bestimmung von Diund Trimethadion im Plasma wird beschrieben. Die Methode beruht auf der Absorptionsbande der Carbonylgruppe bei der Wellenzahl 1740 cm" 1 für Trimethadion und 1770 cm" 1 für Dimethadion. Richtigkeit und Präzision der Methode sind hervorragend; bei einer Di-bzw. Trimethadionkonzentration von 0,5 mmol • 1 beträgt der Variätionskoeffizient <1% und ist besser als der ultraviolettspektrometrischer oder gaschromatographischer Methoden. Die Methode kann zur Bestimmung des Quotienten Trimethadion/Dimethadion als empfindliche Kenngröße für die Zuverlässigkeit des Patienten in der Medikamenteneinnahme während klinischer Therapie eingesetzt werden. Die Bestimmung von Dimethadion kann als Maß für den mittleren intrazellulären pH-Wert im Körper benutzt werden.

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Section: Discussionmentioning

confidence: 99%

Simultaneous Determination of Dimethadione and Trimethadione by Infrared-Spectrometry: Application for Mean Intracellular pH Measurement

Zweens¹,

Frankena²

1984

Clinical Chemistry and Laboratory Medicine

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“…Its acidic form permeates into cells, while its anionic form does not. Changes of DMO concentrations in serum are thus dependent on intracellular pH changes only, DMO should therefore be a sufficient marker of intracellular pH and acid-base balance changes (Waddel and Bates 1969;Albers et al, 1975). Previously described methods for the determination of DMO were based on spectrophotometric measurement, when large doses of DMO were administered (Butler, 1953;Waddel and Butler, 1959), gas-liquid chromatography (Tanaka et al, 1981;Gaidzik and Kmiotek, 1986) or on radioisotope techniques (Saborowski et al, 1973;Tizianello et al, 1977;Albers et al, 1981).…”

Section: Introductionmentioning

confidence: 99%

Rapid and simple HPLC determination of 5,5‐dimethyl‐2,4‐oxazolidynedione in rabbit serum

Gaździk

Filipek

Kmiotek

1987

Biomedical Chromatography

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A new method for the determination of 5,5-dimethyl-2,4-oxazolidynedione (DMO) in rabbit serum by reversed phase-HPLC with UV detection is described. The determination of DMO is performed without derivatisation. The internal standard is 5,5-diethylbarbituric acid (barbital). The method is rapid and simple with sensitivity limit of 20 ng/mL, high recovery (above 92%) and is suitable for pharmacokinetic studies.

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Tissue acid‐base balance and oxygen metabolism in human cerebral infarction studied with positron emission tomography

Syrota¹,

Castaing

Rougemont³

et al. 1983

Annals of Neurology

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Nine patients who had suffered strokes were examined between 10 and 34 days after onset using positron emission tomography. DMO labeled with carbon 11 was used to evaluate brain acid-base balance, and the oxygen-15 inhalation technique was used to measure regional cerebral blood flow, the oxygen extraction fraction, and cerebral metabolic rate for oxygen. [11C]DMO concentration and oxygen metabolism variables were measured in the infarcted area and in the symmetrical region in the contralateral cerebral hemisphere. [11C]DMO concentration was found to be unchanged or slightly increased in five cases and markedly increased in four cases. The apparent increase in tissue pH can be explained by the presence of a large extracellular fluid space with a pH nearly identical to that of brain plasma, or by an increase in intracellular pH, or by both phenomena. The change in [11C]DMO concentration in the infarcted area relative to that in the normal tissue was independent of the change in blood flow. Cerebral metabolic rate for oxygen was decreased in all cases. The increase in [11C]DMO concentration in the infarcted area was linearly correlated with the decrease in the oxygen extraction fraction in the same region; that is, it was correlated with the occurrence of perfusion in excess of metabolic demand. The overabundant local perfusion could play a role in the decreased H+ content.

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Determination of mean whole body entracellular pH in unanesthetized dogs

Cited by 7 publications

References 7 publications

Simultaneous Determination of Dimethadione and Trimethadione by Infrared-Spectrometry: Application for Mean Intracellular pH Measurement

Simultaneous Determination of Dimethadione and Trimethadione by Infrared-Spectrometry: Application for Mean Intracellular pH Measurement

Rapid and simple HPLC determination of 5,5‐dimethyl‐2,4‐oxazolidynedione in rabbit serum

Tissue acid‐base balance and oxygen metabolism in human cerebral infarction studied with positron emission tomography

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