Determination of marbofloxacin in plasma and synovial fluid by ultrafiltration followed by HPLC–MS/MS

Montesano, Camilla; Curini, Roberta; Sergi, Manuel; Compagnone, Darío; Celani, Gianluca; Varasano, Vincenzo; Petrizzi, Lucio; Amorena, Michele

doi:10.1016/j.jpba.2016.01.061

Cited by 13 publications

(9 citation statements)

References 23 publications

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“…The solution of TCA was less efficient for anisomycin extraction from heart tissue (89%, RSD 3%), whereas acetonitrile provided the lowest recovery (69%) with poor method repeatability (RSD 36%). Based on previous reported results summarized in Table 1, methanol generally exhibits good extraction capability, 18,22,33,41 while TCA 28,30,34 and acetonitrile 32,35,36,39 are slightly less efficient for extraction of antibiotics from different biological matrices, which is in accordance with the obtained results.…”

Section: Pharmacokinetic Studysupporting

confidence: 91%

“…Extraction parameters optimized in the study were the extraction solvent and the pH-value of the extract prior to clean-up. Tested extraction solvents were methanol, 18,22,33,38,41 5% solution of TCA 28,30,34,37 and acetonitrile. 19,32,[35][36][37][38][39][40]42 Also, the packing type of the clean-up cartridge was optimized, and Oasis HLB (hydrophilic-lipophilic balance, 200 mg/6 mL, Waters, Milford, MA, USA) 28 and Strata X cartridges 43 (200 mg/6 mL, Phenomenex, Torrance, CA, USA) were tested.…”

Section: Optimization Of the Sample Preparationmentioning

confidence: 99%

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Determination of anisomycin in tissues and serum by LC-MS/MS: application to pharmacokinetic and distribution studies in rats

et al. 2016

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Section: Pharmacokinetic Studysupporting

confidence: 91%

Section: Optimization Of the Sample Preparationmentioning

confidence: 99%

Determination of anisomycin in tissues and serum by LC-MS/MS: application to pharmacokinetic and distribution studies in rats

et al. 2016

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“…All plasma and synovial samples were analysed for marbofloxacin concentrations using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) using rapid sample preparation, which only requires an ultrafiltration step with centrifugal filter devices. 31 Ofloxacin (another fluoroquinolone antibacterial agent) was used as the Internal Standard (IS). Briefly, 175 µl of each sample were mixed with 85 µl of methanol containing IS, at a final concentration of 200 ng/ml in an Eppendorf tube.…”

Section: Methodsmentioning

confidence: 99%

Pharmacokinetics of marbofloxacin administered via intravenous regional limb perfusion in dairy cows: evaluation of two different tourniquets

Celani

Tulini

Montesano

et al. 2017

Veterinary Record Open

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ObjectivesThis study evaluated synovial and systemic plasma pharmacokinetic variables of marbofloxacin after a single intravenous regional limb perfusion (IVRLP) performed using two different tourniquets in clinically healthy, standing, non-sedated dairy cows. The authors hypothesised that the type of tourniquet used for IVRLP would influence the synovial fluid concentration of marbofloxacin in the perfused distal limb.DesignThe study had a randomised parallel-group design.MethodsTen adult dairy cows were included. Unilateral hindlimb IVRLP through the dorsal common digital III vein was performed in two groups of five cows (group 1: wide rubber tourniquet; group 2: manual pneumatic tourniquet) using 0.67 mg/kg of marbofloxacin. The tourniquet was applied proximal to the tarsus and maintained for 30 minutes. Samples of jugular blood and synovial fluid from the tibiotarsal joints of the perfused limb were obtained before and at intervals after IVRLP. All samples were analysed for drug concentrations using liquid chromatography tandem mass spectrometry. Pharmacokinetic parameters were determined to establish the influence of tourniquet types. Differences were considered significant at P≤0.05.ResultsNo adverse effects from the procedure or marbofloxacin were observed in any animal. Significant differences in synovial concentrations and pharmacokinetic parameters were measured. The mean ± sd areas under the concentration versus time curve from time 0 to 24 hours were 178.98±58.08 µg hour/ml for group 2 and 21.11±9.93 µg hour/ml for group 1. The mean ± sd maximum marbofloxacin concentrations were 75.50±10.19 µg/ml for group 2 and 6.35±1.47 µg/ml for group 1.ConclusionsPerforming IVRLP using the dorsal common digital III vein and a manual pneumatic tourniquet set at 300 mmHg above the tarsus in standing cows resulted in significantly higher marbofloxacin concentrations in the tibiotarsal joint compared with those with the wide rubber tourniquet.Trial registrationLocal ethical committee (number 41/2012/CEISA).

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“…This method has important characteristics, such as fast, high throughput, and high specificity, and at the same time, the sample quantity is small, and it can provide spectral information. However, the disadvantage of this method is that the nonspecific combination of small molecules and ultrafiltration membrane will produce false-positive results [40]. Ultrafiltration has been used to screen inhibitors of various enzymes from natural products.…”

Section: Affinity Ultrafiltrationmentioning

confidence: 99%

Recent advances on discovery of enzyme inhibitors from natural products using bioactivity screening

Yan

Cao

2022

J of Separation Science

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The essence of enzymes is to keep the homeostasis and balance of humans by catalyzing metabolic responses and modulating cells. Suppression of an enzyme slows the progress of some diseases, making it a therapeutic target. Therefore, it is important to develop enzyme inhibitors by proper bioactivity screening strategies for the future treatment of some major diseases. In this review, we summarized the recent (2015–2020) applications of several screening strategies (electrophoretically mediated microanalysis, enzyme immobilization, affinity chromatography, and affinity ultrafiltration) in finding enzyme inhibitors from certain species of bioactive natural compounds of plant origin (flavonoids, alkaloids, phenolic acids, saponins, anthraquinones, coumarins). At the same time, the advantages and disadvantages of each strategy were also discussed, and the future possible development direction in enzyme inhibitor screening has been prospected. To sum up, it is expected to help readers select suitable screening strategies for enzyme inhibitors and provide useful information for the study of the biological effects of specific kinds of natural products.

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Determination of marbofloxacin in plasma and synovial fluid by ultrafiltration followed by HPLC–MS/MS

Cited by 13 publications

References 23 publications

Determination of anisomycin in tissues and serum by LC-MS/MS: application to pharmacokinetic and distribution studies in rats

Determination of anisomycin in tissues and serum by LC-MS/MS: application to pharmacokinetic and distribution studies in rats

Pharmacokinetics of marbofloxacin administered via intravenous regional limb perfusion in dairy cows: evaluation of two different tourniquets

Recent advances on discovery of enzyme inhibitors from natural products using bioactivity screening

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