Determination of major carotenoids in human serum by liquid chromatography

Tzeng, Min-Su; Yang, Feili-Lo; Wang-Hsu, Guoo-Shyng; Chen, Bing‐Huei

doi:10.38212/2224-6614.2672

Cited by 11 publications

(9 citation statements)

References 19 publications

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“…Comparing our findings with several other similar studies, only two studies eluted beta-carotene compound with retention times similar with ours, which recorded at 1.2 minutes [13] and three minutes [14], respectively. On the other hand, other researchers documented beta-carotene retention times ranging from 10 to 27 minutes [15][16][17][18][19][20][21][22]. Inter-study variations, such as the types of LC systems applied, types of organic chemicals used in the mobile phase, and types of columns, were some of the factors possibly affecting the retention time of beta-carotene in human sera.…”

Section: Resultsmentioning

confidence: 99%

Protocol of a Rapid Method to Quantify Beta-carotene in Human Sera

Ng¹,

Tan²,

Moy³

2023

Preprint

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Beta-carotene is a fat-soluble antioxidant commonly found in foods such as fruits, vegetables, and palm oil. Despite various liquid chromatography methods are proposed to detect and measure the sera carotenoid level, a long retention time to elute this marker is needed. This study attempted to develop a rapid reverse phase method in eluding beta-carotene in human sera. The researchers managed to elute the antioxidant in 2.2 minutes by applying a combination of a C8 column, mobile phase comprising of acetonitrile mixed with methanol in 70:30 proportion, and an ultra-high performance liquid chromatography system. The outputs had a good calibration curve (R2=0.959) and low coefficient of variation (0.2%), suggesting this protocol is reliable. A column with a lower carbon chain such as C8 allows the beta-carotene molecule to flow through the column faster. Besides, selecting solvents with high elution strength coupled with an ultra-high performance liquid chromatography system which equipped with high pressure can force the beta-carotene through the column in a shorter duration compared to previous reported methods. Therefore, it is recommended to adopt this protocol in epidemiological studies where beta-carotene is screened as a dietary biomarker associated with disease of interest by using human sera in the population level.

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Section: Resultsmentioning

confidence: 99%

Protocol of a Rapid Method to Quantify Beta-carotene in Human Sera

Ng¹,

Tan²,

Moy³

2023

Preprint

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“…Lutein concentrations in serum samples were determined according to the existing protocols [ 35 , 36 ]. In 200 μL of serum, we added 1 mL of deionized water and 0.01% ascorbic acid dissolved in absolute ethanol and stirred the mixture.…”

Section: Methodsmentioning

confidence: 99%

Consumption of Nutritionally Enriched Hen Eggs Enhances Endothelium-Dependent Vasodilation via Cyclooxygenase Metabolites in Healthy Young People—A Randomized Study

et al. 2023

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Objective: The present study aimed to evaluate the effects of enriched hen egg consumption on endothelium-dependent vasodilation (EDV) and the role of cyclooxygenases in EDV in the microcirculation of young healthy individuals. This study hypothesizes that Nutri4 eggs will improve endothelial function, which will be manifested by changes in microcirculatory flow measured by a laser Doppler flowmeter (LDF) during reactive hyperemia in response to vascular occlusion, in which n-3 PUFA plays an important role as well as its degradation pathway by cyclooxygenases. Materials and Methods: Participants consumed three eggs per day for three weeks: The control group (CTRL, n = 14) consumed regular hen eggs (approximately 0.330 mg of lutein, 1.785 mg of vitamin E, 0.054 mg of selenium and 438 mg of n-3 PUFAs daily) and Nutri4 group (n = 20) consumed enriched eggs (approximately 1.85 mg of lutein, 0.06 mg of selenium, 3.29 mg of vitamin E, and 1026 mg of n-3 PUFAs daily). Skin microvascular blood flow in response to EDV (post-occlusive reactive hyperemia (PORH) and iontophoresis of acetylcholine (AChID)) and sodium nitroprusside (SNPID; endothelium-independent) was assessed by laser Doppler flowmetry before and after dietary protocol and in a separate group of participants who were administered perorally 100 mg of indomethacin before microvascular response assessment. Arterial blood pressure, heart rate, serum lipid, and liver enzymes, anthropometric measurements, protein expression of cyclooxygenase 1 (COX-1), cyclooxygenase 2 (COX-2), neuronal nitric oxide synthases (nNOS), inducible nitric oxide synthases (iNOS), and endothelial nitric oxide synthases (eNOS) were measured before and after dietary protocol. Results: PORH and AChID were significantly enhanced, and SNPID remained unchanged in the Nutri4 group, while none was changed in the CTRL following a respective diet. PORH decreased after administration of indomethacin in Nutri4 after dietary protocol. Protein expression of COX-2 was significantly higher in the Nutri4 group compared to the CTRL after the dietary protocol. Conclusion: Consumption of enriched eggs improves microvascular EDV in healthy young subjects. Results suggest an element of n-3 PUFAs metabolites via the cyclooxygenases pathway in enhanced reactive hyperemia.

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“…Lutein concentrations in serum samples were determined according to the protocol by Tzeng et al (2004) [23,24]. Deionized water and 0.01% ascorbic acid (Grammol, GRAM-MOL d.o.o., Zagreb, Croatia) dissolved in absolute ethanol (Gram-mol GRAM-MOL d.o.o., Zagreb, Croatia) was added to serum samples and mixed thoroughly.…”

Section: Measurements Of Fatty Acids Profile Vitamin E Selenium and L...mentioning

confidence: 99%

Consumption of Hen Eggs Enriched with n-3 Polyunsaturated Fatty Acids, Selenium, Vitamin E and Lutein Incites Anti-Inflammatory Conditions in Young, Healthy Participants — A Randomized Study

Šušnjara¹,

Kolobarić²,

Matić³

et al. 2022

Front. Biosci. (Landmark Ed)

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Background: Dietary supplementation with compounds that possess antioxidant and anti-inflammatory properties (n-3 polyunsaturated fatty acids (PUFAs), selenium, vitamin E, lutein), has been shown to positively correlate with improvements in chronic conditions, although understanding of these combined effects in healthy humans is limited. The study aimed to evaluate the effects of enriched eggs consumption on oxidative status and inflammatory conditions in healthy volunteers. We hypothesized that a three-week diet containing enriched eggs can alter the immune response of healthy adults towards anti-inflammatory conditions. Methods: 34 participants consumed 3 hard-boiled hen eggs per day (21 days): Control group-regular hen eggs (n-3 PUFAs = 438 mg, selenium = 0.054 mg, lutein = 0.330 mg and vitamin E = 1.785 mg) (N = 14); 4Nutri group-hen eggs enriched with 4 nutrients (n-3 PUFAs = 1026 mg, selenium = 0.06 mg, lutein = 1.85 mg and vitamin E = 3.29 mg) (N = 20). Samples were taken before and after the protocol. Serum concentrations of lipid mediators and cytokines were measured with enzyme-linked immunosorbent assay (ELISA) and antibody-based, magnetic bead reagent kits on the Luminex platform, respectively. Serum oxidative stress and antioxidant capacity were measured using standardized methods, while gene expression in peripheral blood mononuclear cells (PBMCs) was measured via real-time PCR. Results: Decreased serum levels of pro-inflammatory interleukin 17A (IL-17A) and an increased neuronal nitric oxide synthase (nNOS) expression in the 4Nutri group, together with alteration of metabolites produced via cyclooxygenase (COX) pathways in the Control group, suggest a shift towards anti-inflammatory conditions in participants who consumed enriched hen eggs. Conclusions: Present results suggest that the combined action of n-3 PUFAs and antioxidants may have a protective role in resting, non-inflammatory conditions. Clinical Trial Registration: NCT04564690.

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Determination of major carotenoids in human serum by liquid chromatography

Cited by 11 publications

References 19 publications

Protocol of a Rapid Method to Quantify Beta-carotene in Human Sera

Protocol of a Rapid Method to Quantify Beta-carotene in Human Sera

Consumption of Nutritionally Enriched Hen Eggs Enhances Endothelium-Dependent Vasodilation via Cyclooxygenase Metabolites in Healthy Young People—A Randomized Study

Consumption of Hen Eggs Enriched with n-3 Polyunsaturated Fatty Acids, Selenium, Vitamin E and Lutein Incites Anti-Inflammatory Conditions in Young, Healthy Participants — A Randomized Study

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