Determination of lymphocyte subset reference ranges in peripheral blood of healthy adults by a dual-platform flow cytometry method

Rudolf-Oliveira, Renata Cristina Messores; Gonçalves, Kauê Taneli; Martignago, Mariana Lenhani; Mengatto, Vanessa; Gaspar, Pâmela Cristina; Moraes, Ana Carolina Rabello de; Silva, Rosemeri Maurici da; Bazzo, Maria Luiza; Santos-Silva, Maria Cláudia

doi:10.1016/j.imlet.2014.11.003

Cited by 21 publications

(7 citation statements)

References 34 publications

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“…ACs had significantly increased numbers of Ab-producing plasmablasts (CD27 hi CD38 hi ) along with high anti-N and -S1 IgG ratios ( Figure 1A). The regress of humoral immunity during convalescence was clearly indicated by a decrease of plasmablasts and an increase of transitional, naïve, and marginal zone B cells, coming closer to steady-state conditions found in the healthy population (Rudolf-Oliveira et al, 2015). The development of long-term humoral immunity elicited by SARS-CoV-2 infection was indicated by higher numbers of memory B cells in RCs when compared to ACs.…”

Section: Sars-cov-2 Igg Ratios Correlate With Disease Severity and Dementioning

confidence: 53%

COVID-19 immune signatures reveal stable antiviral T cell function despite declining humoral responses

et al. 2021

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Section: Sars-cov-2 Igg Ratios Correlate With Disease Severity and Dementioning

confidence: 53%

COVID-19 immune signatures reveal stable antiviral T cell function despite declining humoral responses

et al. 2021

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“…Briefly, the following B-cell subpopulations were defined based on their staining profile for CD19, CD38, CD24, CD21, CD27, CD5, surface membrane (sm)IgM, and smIgD: (1) CD27 2 CD38 hi CD24 hi CD5 1 smIgM 11 IgD 1 immature/transitional B-cells, (2) CD27 2 CD38 lo CD24 het CD5 het smIgM MBCs, see Fig E1). Absolute counts were calculated by using total B-cell counts based in a double-platform assay 28 and used throughout the study. Intralaboratory and interlaboratory variability was assessed at the participating centers based on replicate measurements of the same samples to ensure comparable results at distinct sites (see Fig E2 in this article's Online Repository at www.jacionline.org).…”

Section: Flow Cytometric Identification Of Blood B Cells and Their Sumentioning

confidence: 99%

What Are the Characteristics of the Upper Airway in Patients With Craniofacial Microsomia?

Klazen

Caron²,

Schaal³

et al. 2019

Journal of Oral and Maxillofacial Surgery

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IgAdef, B-cell defects were mainly restricted to surface membrane (sm)IgA 1 PCs and MBCs, with 2 clear subgroups showing strongly decreased numbers of smIgA 1 PCs with mild versus severe smIgA 1 MBC defects and higher frequencies of nonrespiratory tract infections, autoimmunity, and affected family members. Patients with IgG subclass deficiency with IgA deficiency and those with CVID showed defects in both smIgA 1 and smIgG 1 MBCs and PCs. Reduced numbers of switched PCs were systematically found in patients with CVID (absent in 98%), with 6 different defective MBC (and clinical) profiles: (1) profound decrease in MBC numbers; (2) defective CD27 1 MBCs with almost normal IgG 3 1 MBCs; (3) absence of switched MBCs; and (4) presence of both unswitched and switched MBCs without and; (5) with IgG 2 1 MBCs; and (6) with IgA 1 1 MBCs. Conclusion: Distinct PAD defective B-cell patterns were identified that are associated with unique clinical profiles. (J

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“…A limitation of flow cytometry is the absence of comprehensive normative data that has kept up with the advances in our understanding of immunologic cell subsets in humans. Reference values for basic lymphocyte subsets, which provide a significant resource for clinical decisions and interpretation of immunological research in general, have been published in multiple populations throughout the world [1][2][3][4][5][6][7][8]. Existing normative data has several limitations including small sample sizes, incompletely described sample populations and flow cytometry techniques, and often limited depth regarding the immune cell populations described.…”

Section: Introductionmentioning

confidence: 99%

Establishment of normative ranges of the healthy human immune system with comprehensive polychromatic flow cytometry profiling

Rosa-Bray

Staats

et al. 2019

PLoS ONE

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Existing normative flow cytometry data have several limitations including small sample sizes, incompletely described study populations, variable flow cytometry methodology, and limited depth for defining lymphocyte subpopulations. To overcome these issues, we defined high-dimensional flow cytometry reference ranges for the healthy human immune system using Human Immunology Project Consortium methodologies after carefully screening 127 subjects deemed healthy through clinical and laboratory testing. We enrolled subjects in the following age cohorts: 18-29 years, 30-39, 40-49, and 50-66 and enrolled cohorts to ensure an even gender distribution and at least 30% non-Caucasians. From peripheral blood mononuclear cells, flow cytometry reference ranges were defined for >50 immune subsets including T-cell (activation, maturation, T follicular helper and regulatory T cell), B-cell, and innate cells. We also developed a web tool for visualization of the dataset and download of raw data. This dataset provides the immunology community with a resource to compare and extract data from rigorously characterized healthy subjects across age groups, gender and race.

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Determination of lymphocyte subset reference ranges in peripheral blood of healthy adults by a dual-platform flow cytometry method

Cited by 21 publications

References 34 publications

COVID-19 immune signatures reveal stable antiviral T cell function despite declining humoral responses

COVID-19 immune signatures reveal stable antiviral T cell function despite declining humoral responses

What Are the Characteristics of the Upper Airway in Patients With Craniofacial Microsomia?

Establishment of normative ranges of the healthy human immune system with comprehensive polychromatic flow cytometry profiling

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