Determination of Lymphocyte Count Necessary for Isolating Bovine Leukaemia Virus (BLV) from BLV‐Infected Cattle and Correlation between Lymphocyte Count and Antibody Titre

Koyama, Hiroyuki; Hohdatsu, Tsutomu; Nagai, Takayuki; Tsubaki, Shiro

doi:10.1111/j.1439-0450.1988.tb00540.x

Cited by 5 publications

(3 citation statements)

References 6 publications

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“…BLV antibodies. The BLV antigen was prepared from FLK/BLV culture fluid, and the BLV antibodies titer was measured by the Ouchterlony method (5).…”

Section: Methodsmentioning

confidence: 99%

“…The cover glass was fixed in acetone after 48 hours of culture. BLV antigen was detected by the direct fluorescent antibody (FA) test using FITC-conjugated bovine anti-BLV serum (5). BLV was isolated from the established cell lines as follows: 1 x 105 CC-81 cells were mixed with 5 x 106 cells of each cell line, and the mixture was cultivated in 3.5-cm dishes.…”

Section: Methodsmentioning

confidence: 99%

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Properties of Nine Continuous B‐Cell Lines Established from Enzootic Bovine Leukosis Tumors

Koyama

Hohdatsu

Satake

et al. 1992

Journal of Veterinary Medicine, Series B

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We established 9 cell lines from 63 tumor cases of enzootic bovine leukosis and studied their properties. Cells of all lines formed small clumps and floated in culture medium, indicating growth. Four of the 9 cell lines were surface immunoglobulin (S1g)-positive, but the remaining 5 line cells were negative for Slg or, if SIg was detected, the percentage of SIg-positive cells was very low. Tests for the properties of the cells with monoclonal antibodies to lymphocytes revealed that the established line cells are B-lymphocytes. Morphological observation also revealed that they had the morphology of B-lymphoblastic cell. The results of E and EAC rosette assay were negative, but 6 of 8 cell lines were positive for EA rosetting. All the 9 cell lines reacted with MoAb C-143, which recognizes the tumorassociated antigen (TAA) of the EBL tumor cell. All 9 cell lines produced bovine leukosis virus (BLV). These results suggest that the 9 cell lines are tumor cells derived from B-lymphocytes of EBL. a) Number of BLV positive dishes; number of dishes tested.

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“…BLV antibodies. The BLV antigen was prepared from FLK/BLV culture fluid, and the BLV antibodies titer was measured by the Ouchterlony method (5).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Properties of Nine Continuous B‐Cell Lines Established from Enzootic Bovine Leukosis Tumors

Koyama

Hohdatsu

Satake

et al. 1992

Journal of Veterinary Medicine, Series B

View full text Add to dashboard Cite

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“…Cattle with a high antibody titre appear to be more important as a source of infection than animals with low antibody titres (Koyama et al., 1988). Removal of all seropositive cattle from a herd quickly reduces the prevalence of BLV infection (Johnson and Kaneene, 1992).…”

Section: Introductionmentioning

confidence: 99%

An Outbreak of Enzootic Bovine Leukosis in Upper Egypt: Clinical, Laboratory and Molecular–Epidemiological Studies

Zaghawa

Beier²,

et al. 2002

Journal of Veterinary Medicine, Series B

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In 1989, 220 Holstein Friesian cattle (212 heifers and eight bulls) were imported from Minnesota, USA, to form a closed dairy herd in Arab El-Aoumar, Assiut, Upper Egypt. In November 1996, some abnormal signs such as loss of weight, decreased milk yield, external lymphadenopathy and decreased appetite were observed on this farm. Serological screening by enzyme-linked immunosorbent assay revealed a seroprevalence of antibodies directed against bovine leukaemia virus (BLV) of 37.7% in cattle under 2 years old and of 72.8% in animals more than 2 years old. Diagnosis was confirmed by the detection of BLV proviral DNA using polymerase chain reaction with primers amplifying a fragment of the env gene. Out of 21 tested leucocyte fractions from individual animals, 15 were positive showing a BLV-specific amplicon of 444 base pairs. Analysis of the amplicons for restriction fragment length polymorphisms and DNA sequencing results allowed the isolates to be typed. Since this was the first recorded case of enzootic bovine leukosis in Upper Egypt, strict quarantine measures were adopted and all serologically positive animals in the herd were culled.

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Distribution and superinfection of bovine leukemia virus genotypes in Japan

et al. 2004

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A study to investigate the types and distribution of bovine leukemia virus (BLV) was conducted on about eight hundred cattle drawn from 53 farms found in 16 prefectures in Japan. Agar gel immunodiffusion (AGID) tests of serum samples and nested-PCR to detect BLV provirus, in peripheral blood leukocytes were performed. To identify genotypes, restriction fragment length polymorphism (RFLP) was performed with a PCR-amplified 444 bp fragment of the env gene using endonucleases. Three genotypes (1, 3, and 5) were dominant in Japan, and were found in 48.3%, 32.7%, and 16.9% of PCR positive cattle, respectively. Of the cattle infected with genotype 1, 84.7% were strongly positive in the AGID test. Similarly, in cattle with genotype 3, 78.9% were strongly positive. However, only 59.1% of cattle with genotype 5 were strong positive. Three cattle showed unusual RFLP patterns and they were found to be infected with more than one genotype. These results suggest that some BLV infected cattle can not induce effective immune reactions and suffer from superinfection by BLV in the field.

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Determination of Lymphocyte Count Necessary for Isolating Bovine Leukaemia Virus (BLV) from BLV‐Infected Cattle and Correlation between Lymphocyte Count and Antibody Titre

Cited by 5 publications

References 6 publications

Properties of Nine Continuous B‐Cell Lines Established from Enzootic Bovine Leukosis Tumors

Properties of Nine Continuous B‐Cell Lines Established from Enzootic Bovine Leukosis Tumors

An Outbreak of Enzootic Bovine Leukosis in Upper Egypt: Clinical, Laboratory and Molecular–Epidemiological Studies

Distribution and superinfection of bovine leukemia virus genotypes in Japan

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