Determination of Luteinizing and Follicle-Stimulating Principles in Castrate and Menopause Urine

Frank, Robert T.; Salmon, Udall J.; Friedman, Reuben

doi:10.3181/00379727-32-8222p

Cited by 17 publications

(7 citation statements)

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“…The demonstration that there is a rise in the urinary excretion of luteinizing hormone in the menopausal state (27) also is in accord with the suggested hypothesis. Other circumstantial evidence that the luteinizing hormone stimulates 17-ketosteroid production by the adrenal cortex is summarized in the following paragraphs.…”

Section: Discussionsupporting

confidence: 84%

Effect of Methyl Testosterone on Urinary 17-Ketosteroids of Adrenal Origin 123

Reifenstein¹,

Forbes²,

Albright³

et al. 1945

J. Clin. Invest.

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Urinary 17-ketosteroids or their precursors are thought to arise from the adrenal cortices and the testes (1). Testosterone propionate is partially excreted as a 17-ketosteroid (2); on the other hand, 17-methyl testosterone is not excreted as such (3 to. 5). It follows that methyl testosterone in contradistinction to testosterone propionate, may be used to study the effect of a testosterone compound on the endogenous production of urinary 17-ketosteroids. The present paper is primarily concerned with the effect of methyl testosterone on the urinary 17-ketosteroids of adrenal origin.It is essential that such studies be carried out on individuals without functioning testicular tissue, since it is highly probable from animal experiments that testosterone inhibits its own endogenous production in the testis (6 to 8). It is difficult to obtain direct data in humans on the effect of testosterone on the urinary 17-ketosteroids of testicular origin. Thus, whereas the administration of methyl testosterone to a male without disease of the adrenals and testes induced a lowered 17-ketosteroid excretion (Figure 1), the entire effect may have been mediated through

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Section: Discussionsupporting

confidence: 84%

Effect of Methyl Testosterone on Urinary 17-Ketosteroids of Adrenal Origin 123

Reifenstein¹,

Forbes²,

Albright³

et al. 1945

J. Clin. Invest.

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“…Pregnancy urine from man, chimpanzee (Pan troglodytes), gorilla (Gorilla gorilla), orangutan (Pongo pygmaeus), baboon (Papio cynocephalus), rhesus monkey (Macaco mulatta), and marmoset (Callithrix jacchus) were prepared using either a modification [5] of an acetone extraction method [8] or by a variation [18] of a kaolin-acetone procedure [1] followed by Sephadex gel filtration [3], These extraction procedures resulted in con centration of urinary gonadotropin ranging from ten-to 100-fold. Urinary concentrates were resuspended in deionized water for testing in radioimmunoassay and biossay.…”

Section: Preparation Of Urinary Extractsmentioning

confidence: 99%

Antigenic Similarities to HCG Subunits among Chorionic Gonadotropins of Nonhuman Primates

Nixon¹,

Chen²,

Hodgen³

1977

J Med Primatol

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Comparison of antigenic similarity between human chorionic gonadotropin (HCG) subunits and the chorionic gonadotropins of six species of nonhuman primates indicates marked similarity of antigenic determinants between both subunits of HCG and the chorionic gonadotropins of chimpanzees, gorillas, and orangutans. Antisera to HCG subunits (a or ß) did not cross-react with the chorionic gonadotropins of baboons, macaques, or marmosets. Because of the relative availability of chimpanzees for laboratory studies, we suggest that chimpanzees may be the optimal nonhuman primate model for determining the advisability of vaccinations in man using conjugates of HCG fragments to achieve fertility control or for suppression of HCG-producing neoplasms.

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“…Crude human urine powders from normal males, normal females, and pregnant females were obtained by acetone precipitation (12). The crude acetone precipitate was suspended in saline and dialyzed against water at 40 C. The Immunoelectrophoresis of HCG preparations with rabbit antiserum against normal male urine extracts failed to demonstrate the five (no.…”

Section: Methodsmentioning

confidence: 99%

“…Hemagglutination and hemagglutination inhibition studies demonstrated Fraction II to contain specific activity (immunological) comparable to HCG preparations (Table I, the rat prostate and accessory organ method showed Fraction I (0.1 mg) to contain no detectable gonadotropic activity (p 0.0001). Fraction II, obtained from each of three lots of HCG and tested separately, contained 4,000 to 6,000 U per mg. 12 The chromatographic procedure has doubled the specific biological activity of Fraction II as compared to the activity in the HCG preparations.…”

Section: Methodsmentioning

confidence: 99%