Determination of lithium in dried blood spots and dried plasma spots by graphite furnace atomic absorption spectrometry: Method development, validation and clinical application

Manfro, Iuri Dias; Tegner, Mariane; Krützmann, Maria Eduarda; Artmann, A.; Brandeburski, Marcos Roberto; Peteffi, Giovana Piva; Linden, Rafael; Antunes, Marina Venzon

doi:10.1016/j.talanta.2020.120907

Cited by 18 publications

(15 citation statements)

References 17 publications

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“…The DBS sampling has some advantages in comparison with the standard venous blood collecting, which include its small collection volume, having painless and easy sample collection with minimal training required, and being stable and transportable at ambient temperatures [ 20 ]. Dried plasma spots (DPS), representative of serum levels, can also be obtained from a finger prick with the use of membranes that filter erythrocytes [ 21 ]. Determination of lithium in dried blood spots (DBS) and dried plasma spots (DPS) by graphite furnace atomic absorption spectrometry (GFAAS) has been investigated [ 21 ].…”

Section: Therapeutic Monitoring Of Lithium Levels In Different Biolog...mentioning

confidence: 99%

Advances in Therapeutic Monitoring of Lithium in the Management of Bipolar Disorder

Sheikh

Qassem

Triantis

et al. 2022

Sensors

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Since the mid-20th century, lithium continues to be prescribed as a first-line mood stabilizer for the management of bipolar disorder (BD). However, lithium has a very narrow therapeutic index, and it is crucial to carefully monitor lithium plasma levels as concentrations greater than 1.2 mmol/L are potentially toxic and can be fatal. The quantification of lithium in clinical laboratories is performed by atomic absorption spectrometry, flame emission photometry, or conventional ion-selective electrodes. All these techniques are cumbersome and require frequent blood tests with consequent discomfort which results in patients evading treatment. Furthermore, the current techniques for lithium monitoring require highly qualified personnel and expensive equipment; hence, it is crucial to develop low-cost and easy-to-use devices for decentralized monitoring of lithium. The current paper seeks to review the pertinent literature rigorously and critically with a focus on different lithium-monitoring techniques which could lead towards the development of automatic and point-of-care analytical devices for lithium determination.

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Section: Therapeutic Monitoring Of Lithium Levels In Different Biolog...mentioning

confidence: 99%

Advances in Therapeutic Monitoring of Lithium in the Management of Bipolar Disorder

Sheikh

Qassem

Triantis

et al. 2022

Sensors

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“… LC-HRMS [ 114 ] VAMS Blood Hydroxychloroquine 10 LC-MS/MS [ 115 ] DBS Blood Hydroxychloroquine n.s. LC-MS/MS [ 116 ] DBS Blood Li + 50 GF-AAS [ 118 ] DPS Plasma Li + 20 GF-AAS [ 118 ] DBS Blood Cyclosporin A n.s. LC-MS/MS [ 120 ] Abbreviations : AAS, Atomic Absorption Spectrometry; BD, Blood Drop (not dried); BFS, BioFluid Sampler; BMS, Bronchoscopic MicroSample; DBS, Dried Blood Spots; DMKS, Dried MilK Spots; DPS, Dried Plasma Spots; DOFS, Dried Oral Fluid Spots; DUS, Dried Urine Spots; EBC, Exhaled Breath Condensate; ELF, Epithelial Lining Fluid; FL, FLuorescence detection; FPSE, Fabric-Phase Sorptive Extraction; GF, Graphite Furnace; HRMS, High-Resolution Mass Spectrometry; LC, Liquid Chromatography or high-performance Liquid Chromatography; MS/MS, tandem Mass Spectrometry; NB, Northern Blotting; n.s., not specified (for BD and DBS it usually ranges from 50 to 200 μL); NSAID, Non-Steroidal Anti-Inflammatory Drug; PCR, Polymerase Chain Reaction; PS, Paper Spray; SB, Southern Blotting; SPE, Solid-Phase Extraction; SPME, Solid-Phase MicroExtraction; UHPLC, Ultra-High-Performance Liquid Chromatography; VAMS, Volumetric Absorptive MicroSampling.…”

Section: Microsampling and Sars-cov-2mentioning

confidence: 99%

“…Lithium salts, usually administered to treat bipolar disorder, have also demonstrated some antiviral activity in preclinical studies [ 117 ]. Lithium requires constant TDM during the therapy, so it is a prime candidate for microsampling application; however, until now just DBS and DPS have been used for this purpose [ 118 ].…”

Section: Microsampling and Sars-cov-2mentioning

confidence: 99%

Quantitative microsampling for bioanalytical applications related to the SARS-CoV-2 pandemic: Usefulness, benefits and pitfalls

Protti

Mandrioli

Mercolini

2020

Journal of Pharmaceutical and Biomedical Analysis

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“…Several analytical methods for the quantification of Li in biological samples have been proposed, including atomic absorption spectrophotometry (AAS) [3], inductively coupled plasma (ICP) optical emission spectroscopy (OES) [4], and ICP mass spectrometry (MS) [5]. The development of ICP-based instrumentation has facilitated the simultaneous quantification of several elements of medical interest with sufficient accuracy (trueness and precision) [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Determination of lithium in human serum by isotope dilution atomic absorption spectrometry

et al. 2021

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The therapeutic dose of lithium (Li) compounds, which are widely used for the treatment of psychiatric and hematologic disorders, is close to its toxic level; therefore, drug monitoring protocols are mandatory. Herein, we propose a fast, simple, and low-cost analytical procedure for the traceable determination of Li concentration in human serum, based on the monitoring of the Li isotope dilution through the partially resolved isotope shift in its electronic transition around 670.80 nm using a commercially available high-resolution continuum source graphite furnace atomic absorption spectrometer. With this technique, serum samples only require acidic digestion before analysis. The procedure requires three measurements—an enriched 6Li spike, a mixture of a certified standard solution and spike, and a mixture of the sample and spike with a nominal 7Li/6Li ratio of 0.82. Lanthanum has been used as an internal spectral standard for wavelength correction. The spectra are described as the linear superposition of the contributions of the respective isotopes, each consisting of a spin-orbit doublet, which can be expressed as Gaussian components with constant spectral position and width and different relative intensity, reflecting the isotope ratio in the sample. Both the spectral constants and the correlation between isotope ratio and relative band intensity have been experimentally obtained using commercially available materials enriched with Li isotopes. The Li characteristic mass (mc) obtained corresponds to 0.6 pg. The procedure has been validated using five human serum certified reference materials. The results are metrologically comparable and compatible to the certified values. The measurement uncertainties are comparable to those obtained by the more complex and expensive technique, isotope dilution mass spectrometry. Graphical abstract

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Determination of lithium in dried blood spots and dried plasma spots by graphite furnace atomic absorption spectrometry: Method development, validation and clinical application

Cited by 18 publications

References 17 publications

Advances in Therapeutic Monitoring of Lithium in the Management of Bipolar Disorder

Advances in Therapeutic Monitoring of Lithium in the Management of Bipolar Disorder

Quantitative microsampling for bioanalytical applications related to the SARS-CoV-2 pandemic: Usefulness, benefits and pitfalls

Determination of lithium in human serum by isotope dilution atomic absorption spectrometry

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