Determination of lipids in biological materials by paper chromatography

Bayfield, R.F.; Barrett, J.; Falk, Richard H.

doi:10.1016/s0021-9673(01)85977-x

Cited by 8 publications

(1 citation statement)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some recent applications of paper chromatography include the work of Jensen (11) who determined the tocopherol content of seaweed and seaweed meal and Bayfield et al who measured the tocopherol content of pastures, feed and feces (12) and blood serum (13) without prior saponification. Jensen used circular chromatography on S & S 18 cm papers impregnated with 20% alumina.…”

Section: Papermentioning

confidence: 99%

Modern procedures for the analysis of tocopherols

Bunnell

1971

Lipids

View full text Add to dashboard Cite

The development of reliable assay methodology for the tocopherols has been an evolutionary process which has required over 40 years to reach its present state. Today the analyst has at his disposal a choice of reliable and accurate methods of analysis for the eight tocopherols now known to exist in nature. The general sequence of procedure and the precautions to be observed are described. After suitable extraction procedures and careful saponification techniques, the tocopherols in the nonsaponifiable fraction can be assayed by a variety of chromatographic procedures including paper, thin layer (TLC), column and gas liquid chromatography (GLC). The current methodology of these procedures is reviewed in some detail with special emphasis on the more frequently used TLC and GLC techniques. The earlier methods of GLC assay separated only the mono‐, di‐ and trimethylated tocopherols but methods are now available which provide separation of all the tocopherols. These developments were made possible by better columns and the use of derivatives of tocopherols. In addition to good separation, the GLC method is the most sensitive available for the quantitation of the tocopherols. Spectrophotometric measurements based on Emmerie Engel type reactions have been most frequently used for the final quantitative analysis of the tocopherols separated by paper, TLC, or column chromatography.

show abstract

Section: Papermentioning

confidence: 99%