Determination of Lethal Dose (LD50) and Effects of Gamma Rays  and Ethyl Methane Sulphonate (EMS) Induced Mutagenesis  in Linseed (Linum usitatissimum L.)

Kumar, Adarsh; Paul, Satish; Thakur, Garima

doi:10.20546/ijcmas.2020.910.313

Cited by 7 publications

(5 citation statements)

References 2 publications

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“…The selection of effective and efficient mutagen is of paramount importance in any mutagenic experiments to obtain promising mutations (Kumar et al, 2021). The usefulness of mutagen depends on the spectrum of chlorophyll mutations, lethality, sterility, mutagenic efficiency and effectiveness of the mutagen.…”

Section: Introductionmentioning

confidence: 99%

Mutagenic effectiveness, efficiency and dose optimization of gamma rays in papaya (Carica papaya L.) varieties

2022

EJPB

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The present study was conducted to determine the mutagenic effectiveness, efficiency and lethal dose of gamma rays in papaya varieties CO7, Arka Prabhath and Arka Surya. Papaya seeds were exposed to various doses of gamma rays (0Gy, 100Gy, 150Gy, 200Gy, 250Gy, 300Gy, 350Gy, 400Gy, 450Gy and 500Gy) and the mutagenic effectiveness and efficiency of gamma rays were assessed based on the chlorophyll mutation frequency and biological damage in M 1 plant basis. There was a negative relationship between mutagenic effectiveness and efficiency with the doses of gamma irradiation. LD 50 value was optimized in CO7, Arka Prabhath and Arka Surya as 354, 348 and 341Gy respectively. Generally, the germination, survival and growth rate of papaya decreased with an increase in gammairradiation doses. Lower doses of mutations created very few mutations and higher doses of gamma irradiation induced lethality and sterility of seedlings whereas, lethal dose 50 has proved to be more effective in inducing desirable mutations in papaya. Since identifying the mutagenic efficiency and effectiveness of the mutagen and optimization of lethal dose is the preliminary step in any mutagenic experiment, this study will offer a foundation for further gamma irradiation studies in papaya to develop desirable mutants.

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Section: Introductionmentioning

confidence: 99%

Mutagenic effectiveness, efficiency and dose optimization of gamma rays in papaya (Carica papaya L.) varieties

2022

EJPB

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“…Kumar et al . [ 35 ] also reported a difference in LD50 values in linseed varieties treated with ethyl methane sulphonate and γ rays. The difference in the LD50 values of the same mutagen may be attributed to differences in genetics, and physiology of the varieties.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Bio-physiological damages and cytological aberrations in cowpea varieties treated with gamma rays and sodium azide

2023

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The assessment of mutagen induced biological damage forms an important study in determining the mutagenic potency and genotypic sensitivity, a vital aspect in mutation breeding programs. A prior assessment of lethal dose (LD50), mutagen induced biological damage (alterations in bio-physiological traits and frequency of cytological aberrations) is a prerequisite for determining an optimum mutagen dose in a successful mutation breeding experiment. Therefore, in a multi-year project of mutation breeding, two widely cultivated varieties of cowpea viz., Gomati VU-89 and Pusa-578, were treated with gamma (γ) rays and sodium azide (SA) doses. The results reflected a proportionate increase in bio-physiological damages with the increase in mutagenic doses and caused a substantial reduction in mean seed germination and seedling height. Different cytological aberrations such as cytomixis, univalents, chromosome stickiness, precocious separation, unequal separation, bridges, laggards, disturbed polarity, dyads, triads, and polyads were observed in both varieties. All the mutagen doses induced a broader spectrum of cytological aberrations with varying frequencies.

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“…Doses of the two extracts were calculated according to [20], and were administered orally. The median lethal doses (LD 50 ) of the ethanolic extracts of P. granatum and O. cusindica fruit peels were determined according to the method described by [21]. The mice were observed for 24 hours, symptoms of toxicity and mortality rates in each group were recorded and LD 50 was calculated for each extract.…”

Section: Biological Investigationsmentioning

confidence: 99%

Antimutagenic and cytotoxic potential of Punica granatum L. and Opuntia ficus-indica L. peels

El Sawi,

Elbatanony,

El-Feky

et al. 2023

Egypt. J. Chem.

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Introduction: This study pointed to validate the phytochemistry, antioxidant and antimutagenic activities of two common plant peels; Punica granatum L. and Opuntia cus-indica L.Material and methods: HPLC analysis was performed for identi cation of phenolics and avonoids, beside to isolation of some triterpenes and phenolics from both plant peels.Results: P. granatum peels composed of 16 avonoids and 18 phenolics, while O. cus-indica comprised of 18 avonoids and 10 phenolics. α-Amyrin acetate (1), friedelin (2), lup-20(29)-en-3β-ol (3), quercetin-3,4'dimethyl ether-7-O-α-Larabinofuranosyl β-D-glucopyranoside (4), punica avanol (5), and two hydrolyzabl tannins (6&7) were isolated from P. granatum peels, while friedelin (8), 24-Methylene-ergosta-5-en-3β-ol (9), apigenin-7-O-glucoside (10), isorhamnetin 3-O-β-D-glucopyranoside (11), and betanin pigment (12) were isolated from O. cus-indica peels. Compounds (1-5 and 8, 10, 11, 12) were isolated for the rst time from both plant peels. P. granatum and O. cus-indica peel extracts have relatively signi cant antioxidant, cytotoxic and antimutagenic effects.Discussion and conclusion: The tested plant peel extracts could be a reliable source as natural antioxidant, antimutagenic and cytotoxic agents with a high level of safety. The novelty of this study is the comparison of such activities of the peels under study.

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Determination of Lethal Dose (LD50) and Effects of Gamma Rays and Ethyl Methane Sulphonate (EMS) Induced Mutagenesis in Linseed (Linum usitatissimum L.)

Cited by 7 publications

References 2 publications

Mutagenic effectiveness, efficiency and dose optimization of gamma rays in papaya (Carica papaya L.) varieties

Mutagenic effectiveness, efficiency and dose optimization of gamma rays in papaya (Carica papaya L.) varieties

Assessment of Bio-physiological damages and cytological aberrations in cowpea varieties treated with gamma rays and sodium azide

Antimutagenic and cytotoxic potential of Punica granatum L. and Opuntia ficus-indica L. peels

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