Determination of lentiviral titer by surface enhanced Raman scattering

Morder, Courtney; Scarpitti, Brian T.; Balss, Karin M.; Schultz, Zachary D.

doi:10.1039/d2ay00041e

Cited by 11 publications

(10 citation statements)

References 39 publications

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“…Together, the demonstration of the E-NoM platform for clinical sample screening that achieved the highly specific, sensitive, and simultaneous measurement of multiviral antigens indicated its potential for future clinical use. The progression from high analytical performance into clinical sample validation on a large patient cohort further distinguished this E-NoM platform from previous reports. − …”

Section: Resultsmentioning

confidence: 79%

“…Different from the previous SERS works for virus detection, this E-NoM platform integrated the highly active anisotropic core–shell nanostructures, the electrohydrodynamic-driven fluid flow, and the NoM configuration for enabling the sensitive signal readout without the reliance on enzymatic amplification, sample filtration, and sophisticated statistical analysis. − …”

Section: Resultsmentioning

confidence: 99%

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High Accuracy of Clinical Verification of Electrohydrodynamic-Driven Nanobox-on-Mirror Platform for Molecular Identification of Respiratory Viruses

Li,

Guan,

Wuethrich

et al. 2024

Anal. Chem.

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The molecular detection of multiple respiratory viruses provides evidence for the rational use of drugs and effective health management. Herein, we developed and tested the clinical performance of an electrohydrodynamic-driven nanobox-on-mirror platform (E-NoM) for the parallel, accurate, and sensitive detection of four respiratory viral antigens. The E-NoM platform uses gold–silver alloy nanoboxes as the core material with the deposition of a silver layer as a shell on the core surfaces to amplify and enable a reproducible Raman signal readout that facilitates accurate detection. Additionally, the E-NoM platform employs gold microelectrode arrays as the mirror with electrohydrodynamics to manipulate the fluid flow and enhance molecular interactions for an improved biosensing response. The presence of viral antigens binds the nanobox-based core–shell nanostructure on the gold microelectrode and creates the nanocavity with extremely strong “hot spots” to benefit sensitive analysis. Significantly, in a large clinical cohort with 227 patients, the designed E-NoM platform demonstrates the capability of screening respiratory infection with achieved clinical specificity, sensitivity, and accuracy of 100.0, 96.48, and 96.91%, respectively. It is anticipated that the E-NoM platform can find a position in clinical usage for respiratory disease diagnosis.

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Section: Resultsmentioning

confidence: 79%

Section: Resultsmentioning

confidence: 99%

High Accuracy of Clinical Verification of Electrohydrodynamic-Driven Nanobox-on-Mirror Platform for Molecular Identification of Respiratory Viruses

Li,

Guan,

Wuethrich

et al. 2024

Anal. Chem.

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“…Prior work has shown that it is possible to detect lentiviruses of similar size to the polystyrene beads using Silmeco substrates. 37 The compatibility between the 3D printed device and various solvents was further studied to determine which types of samples could be analyzed with this device. The experimental details for this experiment are in the ESI.…”

Section: Resultsmentioning

confidence: 99%

A 3D printed sheath flow interface for surface enhanced Raman spectroscopy (SERS) detection in flow

Morder,

Schultz

2024

Analyst

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“…A previously reported homebuilt spectrometer was used to collect Raman spectra. 34 The spectrometer was equipped with a 785 nm laser (Oxxius). The laser was focused onto the samples through a 40× water immersion objective (numerical aperture (NA) = 0.8, Olympus), and the Raman scattering was collected through this same objective before being directed to an Isoplane SCT-320 spectrograph with a ProEM 1600 2 eXcelon3 charge-coupled device detector (Princeton Instruments).…”

Section: Methodsmentioning

confidence: 99%

“…The substrates were placed into a small petri dish with 3 mL of a 5 mM ethanolic solution of MBA to soak for at least 18 h. The substrates were then removed and rinsed with 3 mL of ethanol and ultrapure water before placing the substrates into a three-dimensional (3D)-printed flow cell. 34…”

Section: Substrate Functionalizationmentioning

confidence: 99%

Bleach Cleaning of Commercially Available Gold Nanopillar Arrays for Surface-Enhanced Raman Spectroscopy (SERS)

Morder,

Schorr,

Balss

et al. 2023

Appl Spectrosc

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Surface-enhanced Raman spectroscopy (SERS) is a highly sensitive technique that can assist in trace analysis for biomedical, diagnostic, and environmental applications. However, a major limitation of SERS is surface contamination of the substrates used, which can complicate the spectral reproducibility, limits of detection, and detection of unknown analytes. This is especially prevalent with commercially available substrates as shipping under a controlled and clean environment is difficult. Here we report a method using dilute bleach solutions to remove surface contamination from commercially available substrates consisting of gold-coated nanopillar arrays that maintains functionality. The results show that this method can be used to remove background signals associated with typical surface contamination in commercially available substrates as well as remove thiolated self-assembled monolayers (SAMs). Results indicate the bleach oxidizes the surface contaminants, which can then be easily washed away. Although the metallic surface also becomes oxidized in this process, the surface can be reduced without loss of SERS activity. The SERS intensity of SAMs improved following bleach treatment across all concentrations studied.

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Determination of lentiviral titer by surface enhanced Raman scattering

Abstract: Lentiviruses are commonly used to deliver genetic code into host cells for biomedical applications, such as gene therapy, pharmaceuticals, and vaccine development. Knowing the infectious titer of these virus particles...

Cited by 11 publications

References 39 publications

High Accuracy of Clinical Verification of Electrohydrodynamic-Driven Nanobox-on-Mirror Platform for Molecular Identification of Respiratory Viruses

High Accuracy of Clinical Verification of Electrohydrodynamic-Driven Nanobox-on-Mirror Platform for Molecular Identification of Respiratory Viruses

A 3D printed sheath flow interface for surface enhanced Raman spectroscopy (SERS) detection in flow

Bleach Cleaning of Commercially Available Gold Nanopillar Arrays for Surface-Enhanced Raman Spectroscopy (SERS)

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