Determination of leaf rust resistance genes Lr10, Lr26 and Lr37 by molecular markers in wheat cultivars registered in the Czech Republic

Abstract

Twenty-seven winter wheat cultivars registered in the Czech Republic were tested by molecular markers for the presence of Lr26 and Lr37, and twenty-eight cultivars for the presence of Lr10. Gene Lr37 was determined in eleven cultivars, gene Lr10 in ten cultivars and gene Lr26 in four cultivars. Eight cultivars had combinations of two Lr genes, one cultivar possessed all the three Lr genes. The results of marker analyses were compared with multipathotype analysis which confirmed the presence of Lr26 but did not enable the verification of the presence of Lr10 and Lr37. Seedling resistance was compared with resistance of the studied cultivars in the field.

Virulence surveys of wheat leaf rust in the Czech Republic and resistance genes in registered cultivars

“…Results were published in 17 papers that are available from the authors. Leaf rust resistance genes (Lr) in wheat cultivars registered in Czechoslovakia/Czech Republic were determined in multi-pathotype tests, later they were determined by molecular markers for Lr10, Lr26 and Lr37 (Hanzalová et al 2009). Results were mostly summarized in the papers on physiologic specialization of leaf rust mentioned above.…”

Multiplex PCR assay to detect rust resistance genes Lr26 and Lr37 in wheat

“…Therefore marker-assisted selection employed in this case may be very useful, although the markers need not be effective in all cases (Robert et al 2000;Helguera et al 2003). The presence of Lr37 in the cultivars Barryton and Kodex (Hanzalová et al 2009) was not confirmed by our analyses. High levels of field resistance have been recorded in all tested cultivars possessing this gene (Table 1), except the cultivar Bakfis, where the level of field resistance has been classified at a rating of 5 (medium resistance).…”

“…Near isogenic T. aestivum lines possessing the genes Lr26 and Lr37 in a Thatcher background were used as a positive control. *According to Hanzalová et al (2009); in brackets: data not confirmed by our results; **according to data from the Central Institute for Supervising and Testing in Agriculture of the Czech Republic; scale 1-9, 1 -susceptible, 9 -resistant Methodology and results of greenhouse tests were described in detail by Hanzalová et al (2009).…”

“…Many of these cultivars have been registered in the Czech Republic and the presence of the gene Lr37 was verified by field tests, greenhouse tests and by means of molecular markers (Ambrozková et al 2002;Bartoš et al 2004;Hanzalová et al 2009). The gene Lr37 provides resistance at the adult plant stage and for that reason it is rarely effective at the seedling stage.…”

Sixty years of the Crop Research Institute and its impact on plant breeding

“…At present, molecular markers are also tested for the determination of resistance genes. (Hanzalová et al 2009;Sumíková & Hanzalová 2010).…”