Determination of lansoprazole in biological fluids and pharmaceutical dosage by HPLC

Abstract

A simple and rapid (extractionless) high-performance liquid chromatographic method with UV detection at 230 nm was developed for the determination of lansoprazole in biological fluids and pharmaceutical dosage. Niflumic acid was added as internal standard. The separation was performed at ambient temperature on a C18 Spherisorb column with acetonitrile + 0.1 M sodium acetate (40:60, v/v, pH 7) as mobile phase. The retention time was 5.2 min for niflumic acid and 6.7 min for lansoprazole. The detection limit was 20 ng/ml using a 100 microl loop. The method was successfully applied to a pharmacokinetic study of lansoprazole in humans.

The determination of lansoprazole in pharmaceutical preparation by capillary electrophoresis

“…These results are rather higher than those obtained by the HPLC studies [7,8,11]. These results are rather higher than those obtained by the HPLC studies [7,8,11].…”

Determination of lansoprazole in pharmaceutical capsules by flow injection analysis using UV-detection

“…This method is considerable time saver when comparing to the previous methods such as the spectrophotometric, chromatographic, capillary electrophoretic and electrochemical methods [3][4][5][6][7][8][9][10][11] and has the superiority at least ninety injections per hour which is very appreciable number for routine analysis laboratories. It also offers simple instrumentation and lower running cost.…”

“…The methods for the determination of LNS in pharmaceutical preparation such as HPTLC [3], HPLC [4,5], spectrophotometry [6,7], capillary electrophoresis [8], polarography [9], adsorptive stripping square-wave and anodic voltammetric assay [10,11] have been reported. According to our best of knowledge, there is no flow injection analysis (FIA) covering the determination of LNS appeared in the literature.…”

Validation of HPLC and FIA Spectrophotometric Methods for the Determination of Lansoprazole in Pharmaceutical Dosage Forms and Human Plasma

“…A survey of literature has revealed several analytical methods for the determination of lansoprazole in biological fluids and in pharmaceutical products. These include; high-performance liquid chromatography (HPLC) [3][4][5][6][7], electrochemical [8][9][10] and spectrophotometric methods [11][12][13]. The literature reports few spectrophotometric methods for the quantitation of lansoprazole.…”