Determination of Lactoferrin in Goat Milk by HPLC Method

Abstract

Abstract:The aim of this study was the determination of lactoferrin in goat milk using HPLC method. Milk samples were collected at a goat farm in the South Moravia Region, the Czech Republic. It were established bulk tank samples of raw milk (n = 24) and pasteurised milk (n = 27) that were collected during lactation. Lactoferrin contents were analysed by reverse phase high-performance liquid chromatography (RP-HPLC) with diode-array detector PDA 2996. Detection was carried out at the wavelength 205 nm. The average concentration of lactoferrin in goat milk was 120 ± 18 µg/ml. The lactoferrin content was increasing within the lactation period in the ranges of 98 ± 170 µg/ml in April to 149 ± 19 µg/ml in November. The heat treatment (pasteurisation at 72°C for 20 s) resulted in no significant effect on the lactoferrin content. No statistically significant differences (P = 0.05) were found between the values of raw and pasteurised goat milk.

Effect of different treatments on the stability of lysozyme, lactoferrin and β‐lactoglobulin in donkey's milk

“…Similar results for lactoferrin stability in raw goat's milk were reported by Dračková et al . (). The authors observed maximum lactoferrin concentration in colostrum, while over the following weeks the concentration decreased.…”

Applicability of a Human Lactoferrin in Pediatric Practice

“…Продукция рчЛф с моло-ком коз составила до 10 г/л [44][45][46]. Важно отметить, что козы не имеют общих с человеком заболеваний.…”

Recombinant lactoferrin (Lf) of Vechur cow, the critical breed of Bos indicus and the Lf gene variants

“…The eluted protein was dialyzed against PBS containing decreasing concentration of urea. Purity of the recombinant Lf was checked by HPLC following the method of Drakova et al (2009).…”