Determination of L-Phenylalanine in Human Plasma Samples with New Fluorometric Method

Sarı, Tolga; Dede, Süreyya; Yusufoğlu, Büşra; Karakuş, Emine

doi:10.1007/s12010-021-03694-7

Cited by 7 publications

(3 citation statements)

References 31 publications

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“…Moreover, due to the energy loss, the wavelength of the emitted fluorescence shifts to a lower energy (longer wavelength) than the excitation wavelength (Stokes shift) [60]. Ammonia detection using the fluorometric method employs o-phthaldialdehyde (OPA) and sulfite as the reagents, which react with ammonia forming a highly fluorescent isoindole derivative (Equation ( 13)) [61][62][63][64]. The fluorescent product has maximum excitation and emission wavelengths at 362.5 and 423.0 nm, respectively (Figure 7b) [63,65].…”

Section: Fluorometric Methodsmentioning

confidence: 99%

Quantification Methodology of Ammonia Produced from Electrocatalytic and Photocatalytic Nitrogen/Nitrate Reduction

Utomo

2022

Energies

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Nitrogen reduction reaction (NRR) and nitrate reduction reaction (NO3−RR) provide a potential sustainable route by which to produce ammonia, a next-generation energy carrier. Many studies have been conducted over the years, mainly emphasizing material design and strategies to improve catalytic performance. Despite significant achievements in material design and corresponding fundamental knowledge, the produced ammonia is still very limited, which makes it prone to bias. The presence of interferants (e.g., cations and sacrificial reagents), the pH of the solution, and improper analytical procedure can lead to the over or underestimation of ammonia quantification. Therefore, the selection of the appropriate ammonia quantification method, which meets the sample solution condition, along with the proper analytical procedures, is of great importance. In this review, the state-of-the-art ammonia quantification method is summarized, emphasizing the advantages, limitations, and practicality for NRR and NO3−RR studies. Fundamental knowledge of the quantification method is introduced. Perspective on the considerations for selecting the suitable quantification method and for performing the quantification process is also provided. Although non exhaustive, this focused review can be useful as a guide to design the experimental setup and procedure for more reliable ammonia quantification results.

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Section: Fluorometric Methodsmentioning

confidence: 99%

Quantification Methodology of Ammonia Produced from Electrocatalytic and Photocatalytic Nitrogen/Nitrate Reduction

Utomo

2022

Energies

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“…Phenylalanine ammonia lyase (PAL) was the first investigated enzyme. It converts Phe to trans -cinnamic acid, producing a quantitative amount of ammonia, which can be detected by direct optical measurements ( Sari et al, 2022 ) through an easy color change of the pH indicator ( Villalonga et al, 2008 ) or by capacitive measurements using zirconia oxide nanostructures ( Ando et al, 2022 ). The PDH is the most investigated NAD-dependent enzyme used for the development of enzymatic-analytical assays for Phe recognition.…”

Section: Introductionmentioning

confidence: 99%

A highly sensitive colorimetric approach based on tris (bipyridine) Ruthenium (II/III) mediator for the enzymatic detection of phenylalanine

et al. 2023

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The accurate monitoring of phenylalanine concentration plays a prominent role in the treatment of phenylketonuria (PKU). In this study, we present an enzymatic assay based on Phenylalanine Dehydrogenase/NAD+ and tris (bipyridine) Ruthenium (II/III) as a colorimetric mediator for the detection of Phenylalanine concentration. The amount of amino acid was quantitatively recognized by optical absorption measurements at 452 nm through the conversion of Ru (byp)33+ to Ru (byp)32+, which is induced by the neoformed NADH. A detection limit of 0.33 µM, a limit of quantification of 1.01 µM, and a sensitivity of 36.6 a.u nM−1 were obtained. The proposed method was successfully tested using biological specimens from patients affected by hyperphenylalaninemia. The proposed enzymatic assay showed a high selectivity, making it a promising alternative for the development of versatile assays for the detection of phenylalanine in diluted serums.

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“…The accumulation of L-Phe in the brain leads to mental development disorders. [2][3][4] This inherited disease has been known as phenylketonuria (PKU). Untreated or late-treated PKU has been associated with developmental disorders of the nervous system, mental deficiency, and abnormal appearance disorders such as microcephaly (small-headedness).…”

Section: Introductionmentioning

confidence: 99%

An ECL sensor combined with a paper electrode for the determination of phenylalanine

Torul

Kayiş

BoyacÎ¹

et al. 2022

Analyst

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Determination of L-Phenylalanine in Human Plasma Samples with New Fluorometric Method

Cited by 7 publications

References 31 publications

Quantification Methodology of Ammonia Produced from Electrocatalytic and Photocatalytic Nitrogen/Nitrate Reduction

Quantification Methodology of Ammonia Produced from Electrocatalytic and Photocatalytic Nitrogen/Nitrate Reduction

A highly sensitive colorimetric approach based on tris (bipyridine) Ruthenium (II/III) mediator for the enzymatic detection of phenylalanine

An ECL sensor combined with a paper electrode for the determination of phenylalanine

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