Determination of L-Ascorbic Acid Using MBs-AOX/GO/IGZO/Al by Wireless Sensing System and Microfluidic Framework

Abstract

In this paper, the L-ascorbic acid (L-AA) biosensor based on magnetic beads-ascorbate oxidase/graphene oxide/indium gallium zinc oxide/aluminum (MBs-AOX/GO/IGZO/Al) membrane was integrated with the potentiometric measurement system with the microfluidic framework in order to investigate the different sensing characteristics under the static condition and the dynamic condition. The L-AA biosensor showed the average sensitivity of 78.9 mV/decade (25 • C) under the static condition, and it showed the average sensitivity of 81.7 mV/decade (25 • C) at the optimal flow rate (25 µL/min). Besides, the XBee module was used to apply in the remote detection for the L-AA biosensor, and the experimental results were shown the average sensitivity of 78.7 mV/decade (25 • C). Moreover, we also investigated various experiments, such as temperature effect, hysteresis effect, and lifetime. These experimental results would be beneficial to the development of the L-AA biosensor based on electrochemical detection. INDEX TERMS L-ascorbic acid (L-AA), graphene oxide (GO), magnetic beads (MBs), microfluidic framework, wireless sensing system.

Rapid Selective Detection of Ascorbic Acid Using Graphene-Based Microfluidic Platform

“…Moreover, microfluidic technology offers integration of various components in a single platform [16]. Therefore, flexibility, rapid analysis, low fabrication costs, ease of implementation and disposability promote microfluidic technology to a very popular solution in biochemical sensing [17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36]. AA is soluble in water, allowing the application of food microfluidics.…”

“…The graphene/CuPc/PANI nanocomposite structure has been manufactured by electrolytic exfoliation technique for the determination of AA and investigation of its electrochemical characteristics [29], [30]. The complete measurement system based on the complex structure of the magnetic beads-ascorbate oxidase/graphene oxide/indium gallium zinc oxide/aluminium and the microfluidic device has been developed and tested as a biosensor of AA [31]. An AA sensor validated by means of a commercial vitamin C supplement, applying a structure based on pyrolysed photoresist films and graphene nano-sheets, has been demonstrated [32].…”