Determination of Isotianil in Brown Rice and Soil Using Supercritical Fluid Extraction and Gas Chromatography/Mass Spectrometry

Yoshida, Tatsuo; Itou, Asami; Yamamoto, Rise; Tobino, Toshiaki; Murakawa, Hiroshi; Toda, Kei

doi:10.2116/analsci.29.919

Cited by 6 publications

(3 citation statements)

References 13 publications

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“…Moreover, Isotianil does not show any direct antimicrobial activity against bacteria and fungi ( Ogava et al, 2011 ; Toquin et al, 2012 ). In 2010, it was registered under the name Stout ® in Japan and China, where it substantially increased rice production ( Ogava et al, 2011 ; Brozek et al, 2012 ; Yoshida et al, 2013 ). Its efficiency against rice blast seems unusually high, as lower dosages of Isotianil are needed than of any other existing plant defense activator, such as PBZ and TDL ( Ogava et al, 2011 ).…”

Section: Functional Analogs Of Salicylic Acidmentioning

confidence: 99%

Synthetic plant defense elicitors

2015

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To defend themselves against invading pathogens plants utilize a complex regulatory network that coordinates extensive transcriptional and metabolic reprogramming. Although many of the key players of this immunity-associated network are known, the details of its topology and dynamics are still poorly understood. As an alternative to forward and reverse genetic studies, chemical genetics-related approaches based on bioactive small molecules have gained substantial popularity in the analysis of biological pathways and networks. Use of such molecular probes can allow researchers to access biological space that was previously inaccessible to genetic analyses due to gene redundancy or lethality of mutations. Synthetic elicitors are small drug-like molecules that induce plant defense responses, but are distinct from known natural elicitors of plant immunity. While the discovery of some synthetic elicitors had already been reported in the 1970s, recent breakthroughs in combinatorial chemical synthesis now allow for inexpensive high-throughput screens for bioactive plant defense-inducing compounds. Along with powerful reverse genetics tools and resources available for model plants and crop systems, comprehensive collections of new synthetic elicitors will likely allow plant scientists to study the intricacies of plant defense signaling pathways and networks in an unparalleled fashion. As synthetic elicitors can protect crops from diseases, without the need to be directly toxic for pathogenic organisms, they may also serve as promising alternatives to conventional biocidal pesticides, which often are harmful for the environment, farmers and consumers. Here we are discussing various types of synthetic elicitors that have been used for studies on the plant immune system, their modes-of-action as well as their application in crop protection.

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Section: Functional Analogs Of Salicylic Acidmentioning

confidence: 99%

Synthetic plant defense elicitors

2015

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“…Although isotianil does not have any direct antimicrobial activity against bacteria and fungi, it can induce the defense response of various plants to pathogens. For example, isotianil treatment can induce the expression of some defenserelated genes, such as NPR1 and PR1 in the SA signaling pathway (Yoshida and Toda, 2013;Bektas and Eulgem, 2014). There is only one paten report on the application of isotianil in FWB before (Gilbert et al, 2019), and the specific mechanism of isotianil inducing plant resistance is also unclear yet.…”

Section: Introductionmentioning

confidence: 99%

Disentangling the resistant mechanism of Fusarium wilt TR4 interactions with different cultivars and its elicitor application

Zhou

He²,

Tian³

et al. 2023

Front. Plant Sci.

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Fusarium wilt of banana, especially Tropical Race 4 (TR4) is a major factor restricting banana production. Developing a resistant cultivar and inducing plant defenses by elicitor application are currently two of the best options to control this disease. Isotianil is a monocarboxylic acid amide that has been used as a fungicide to control rice blast and could potentially induce systemic acquired resistance in plants. To determine the control effect of elicitor isotianil on TR4 in different resistant cultivars, a greenhouse pot experiment was conducted and its results showed that isotianil could significantly alleviate the symptoms of TR4, provide enhanced disease control on the cultivars ‘Baxi’ and ‘Yunjiao No.1’ with control effect 50.14% and 56.14%, respectively. We compared the infection processes in ‘Baxi’ (susceptible cultivars) and ‘Yunjiao No.1’ (resistant cultivars) two cultivars inoculated with pathogen TR4. The results showed that TR4 hyphae could rapidly penetrate the cortex into the root vascular bundle for colonization, and the colonization capacity in ‘Baxi’ was significantly higher than that in ‘Yunjiao No.1’. The accumulation of a large number of starch grains was observed in corms cells, and further analysis showed that the starch content in ‘Yunjiao No. 1’ as resistant cultivar was significantly higher than that in ‘Baxi’ as susceptible cultivar, and isotianil application could significantly increase the starch content in ‘Baxi’. Besides, a mass of tyloses were observed in the roots and corms and these tyloses increased after application with isotianil. Furthermore, the total starch and tyloses contents and the control effect in the corms of ‘Yunjiao No.1’ was higher than that in the ‘Baxi’. Moreover, the expression levels of key genes for plant resistance induction and starch synthesis were analyzed, and the results suggested that these genes were significantly upregulated at different time points after the application of isotianil. These results suggest that there are significant differences between cultivars in response to TR4 invasion and plant reactions with respect to starch accumulation, tyloses formation and the expression of plant resistance induction and starch synthesis related genes. Results also indicate that isotianil application may contribute to disease control by inducing host plant defense against TR4 infection and could be potentially used together with resistant cultivar as integrated approach to manage this destructive disease. Further research under field conditions should be included in the next phases of study.

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“…tomato DC3000 (Pst DC3000) [17]. Isotianil (ISO) is able to induce resistance to rice blast at a low dose and shows good efficacy without direct antifungal activity [18]. Other reported plant elicitors include N-cyanomethyl-2chloroisonicotinamide (NCI), 3-chloro-1-methylpyrazole-5-carboxylic acid (CMPA), and so on [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Plant Defense Responses to a Novel Plant Elicitor Candidate LY5-24-2

Chen

et al. 2022

IJMS

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Plant elicitors enhance plant defense against pathogen attacks by inducing systemic acquired resistance (SAR) with no or low direct fungicidal activity. Here we report the synthesis of a novel plant elicitor candidate LY5-24-2 [3,4-dichloro-N-(3-chloro-5-(trifluoromethyl)pyridin-2-yl)isothiazole-5-carboxamide] and evaluation of its SAR inducing activity. Bioassays indicated that LY5-24-2 did not show significant anti-fungal activity but provided long-lasting resistance in Arabidopsis thaliana (A. thaliana) through promoting the accumulation of lignin, cellulose and pectin by 60.1%, 82.4% and 305.6%, respectively, at a concentration of 100 µM. LY5-24-2 also facilitated the closure of leaf stomata and increased the intracellular free Ca2+ by 47.8%, induced reactive oxygen species (ROS) accumulation, and inhibited the activity of ascorbate peroxidase (APX, EC 1.11.1.11) and catalase (CAT, EC 1.11.1.6) by 38.9% and 34.0%, respectively, as compared with the control at a concentration of 100 µM. LY5-24-2 induced SAR in plants and was dependent on the NPR1-mediated SA pathway by up-regulating expression of 2273 genes in A. thaliana. Meanwhile, LY5-24-2 also improved cucumber (Cucumis sativus L.) defense against Pseudoperonospora cubensis (P. cubensis) through promoting ROS accumulation and inhibiting activity of APX and CAT by 30.7% and 23.1%, respectively. Its expression of SA signaling genes CsNPR1, CsPR4 and CsPR5 was enhanced by 10.8, 5.8 and 6.6 times, respectively. These results demonstrated that LY5-24-2 is a novel elicitor candidate for plant protection via inducing SAR.

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Determination of Isotianil in Brown Rice and Soil Using Supercritical Fluid Extraction and Gas Chromatography/Mass Spectrometry

Cited by 6 publications

References 13 publications

Synthetic plant defense elicitors

Synthetic plant defense elicitors

Disentangling the resistant mechanism of Fusarium wilt TR4 interactions with different cultivars and its elicitor application

Plant Defense Responses to a Novel Plant Elicitor Candidate LY5-24-2

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