Determination of isoprostane 8‐iso‐PGF2α and prostaglandin GF2α in plasma and intestine of specific-pathogen-free chickens challenged with Eimeria maxima
Abstract:The purpose of this study was to evaluate and determine the concentration of isoprostane 8‐iso‐PGF2α and prostaglandin GF2α (PGF2α) from plasma and intestine in specific pathogen-free (SPF) chickens challenged with Eimeria maxima (EM) using solid‐phase microextraction and ultra‐performance liquid chromatography/tandem mass spectrometry. Forty one-day-old male SPF chickens were randomly allocated to one of two groups with four replicates (n=5 chickens/replicate). Groups consisted of Control (no challenge) or th… Show more
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