Determination of isoflavones and coumestrol in river water and domestic wastewater sewage treatment plants

Bacaloni, Alessandro; Cavaliere, Chiara; Faberi, Angelo; Foglia, Patrizia; Samperi, Roberto; Laganà, Aldo

doi:10.1016/j.aca.2004.10.037

Cited by 63 publications

(66 citation statements)

References 14 publications

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“…However, some limitations of using ISs should also be taken into account (e. g., difficult selection of adequate IS, its comigration with analytes, etc.). Noteworthy, the present CE-DAD method permitted the resolution of the three main aglycone soybean isoflavones (daidzein, glycitein, and genistein) plus kampferol in a relatively short time (within 16 min) with respect to published HPLC methods based on gradient separation that can require up to 55 min although in general with better sensitivity [3,30,37].…”

Section: Flavonoid Content In Legume Sprouts and Effects Of Uv-b Treamentioning

confidence: 92%

Compositional changes induced by UV‐B radiation treatment of common bean and soybean seedlings monitored by capillary electrophoresis with diode array detection

Dinelli¹,

Aloisio²,

Bonetti³

et al. 2007

J of Separation Science

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In this work, a new CE method with diode array detection (DAD) was developed for the monitoring and quantitation of flavonoids in different beans treated and untreated with UV-B radiation. Flavonoid concentration was monitored in UV-B-treated and untreated sprouts of three common beans (Zolfino ecotype, cv. Verdone, cv. Lingua di Fuoco) and one soybean (cv. Pacific). After acid hydrolysis of extracts, the CE-DAD method provides reproducible quantitative determinations of daidzein, glycitein, genistein, and kaempferol at ppm level in these natural matrices within a relatively short time (less than 16 min). Total flavonoid content determined by CE-DAD was 159 +/- 8, 26 +/- 2, 13 +/- 1, and 1.3 +/- 0.3 microg/g fresh weight for untreated sprouts of Pacific soybean, Verdone bean, Zolfino bean, and Lingua di Fuoco bean, respectively. UV-B treatment caused no significant quantitative effect on Pacific soybean sprouts, whereas it enhanced the total isoflavone content by 1.5, 1.8, and 3.2-fold in Verdone, Zolfino, and Lingua di Fuoco beans, respectively. The proposed method shows (i) the potentialities of bean sprouts as a natural source of bioactive compounds (antioxidants); (ii) the technological role of UV-B treatment for sprout isoflavone enrichment; and (iii) the good capabilities of CE-DAD to monitor this process.

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Section: Flavonoid Content In Legume Sprouts and Effects Of Uv-b Treamentioning

confidence: 92%

Compositional changes induced by UV‐B radiation treatment of common bean and soybean seedlings monitored by capillary electrophoresis with diode array detection

Dinelli¹,

Aloisio²,

Bonetti³

et al. 2007

J of Separation Science

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“…[14,15] When tandem mass spectrometry (MS/MS) is used, higher confidence and lower detection limits can be achieved. [16,17] Sampling is a crucial step in water quality assessment. The use of conventional sampling techniques, based on spot collection of samples, does not provide in most cases appropriate information for assessing the presence of micropollutants in aquatic environments, especially when analyte concentrations fluctuate in time due to occasional releases or environmental conditions.…”

Section: Introductionmentioning

confidence: 99%

Determination of endocrine‐disrupting compounds in drinking waters by fast liquid chromatography–tandem mass spectrometry

et al. 2010

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Growing attention has been recently paid to safety of food and drinking water, making necessary the adoption of policies for water sources protection and the development of sensitive and rapid analytical methods to identify micropollutants. Endocrine-disrupting compounds (EDCs) have emerged as a major issue as they alter the functioning of the endocrine system. Since ingestion of EDCs via food is considered the major exposure route, there is a growing interest in understanding EDC fate during drinking water treatment and in monitoring potential contamination of surface waters and groundwaters. In this work, a fast liquid chromatography-electrospray ionization-tandem mass spectrometry method was developed for the determination of 4-n-nonylphenol (NP), bisphenol A (BPA), estrone (E1), 17β-estradiol (E2) and 17α-ethinylestradiol (EE2) in drinking waters. In the literature analytical articles seldom provide details regarding fragmentation pathways. In this paper spectra of the five EDCs in negative ESI were interpreted with the support of accurate mass spectra acquired by a quadrupole time-of-flight instrument; fragmentation pathways were also proposed. The chromatographic separation of EDCs was optimized on a Pinnacle DB Biphenylic column with a water-acetonitrile gradient. Quantitative analysis was performed in multiple reaction monitoring (MRM) mode using bisphenol A-d(16) (BPA-d(16)) as internal standard; calibration curves showed good correlation coefficients (0.9989-0.9997). All figures of merit of the method were satisfactory; limits of detection were in the range 0.2-0.4 ng/ml. The method was applied to the determination of the analytes in waters sampled by polar organic chemical integrative samplers in a drinking water treatment plant. Rather low concentration of BPA, NP and E1 were measured in the inlet, while none of the considered EDCs was detected in the outlet.

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“…Coumestrol has been recognised as a phytoestrogen owing to its high affinity for ERs, its affinity being approximately 30-100 times stronger than that of the soybean isoflavonoids. It exhibits antagonist functions in humans by competing with endogenous oestrogens for ERs (Bacaloni et al, 2005).…”

Section: Coumestan Levels In Foodmentioning

confidence: 99%

Phytoestrogens in foods on the Nordic market

Forslund¹,

Andersson²

2017

TemaNord

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Determination of isoflavones and coumestrol in river water and domestic wastewater sewage treatment plants

Cited by 63 publications

References 14 publications

Compositional changes induced by UV‐B radiation treatment of common bean and soybean seedlings monitored by capillary electrophoresis with diode array detection

Compositional changes induced by UV‐B radiation treatment of common bean and soybean seedlings monitored by capillary electrophoresis with diode array detection

Determination of endocrine‐disrupting compounds in drinking waters by fast liquid chromatography–tandem mass spectrometry

Phytoestrogens in foods on the Nordic market

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