Determination of iron species in wine by ion-exchange chromatography-flame atomic absorption spectrometry

Abstract

The direct coupling of ion-exchange chromatography to flame atomic absorption spectrometry (AAS) has been achieved by employing a Babington type nebuliser. The system enables all the processes on the column to be followed directly at flow-rates of between 1 and 5 ml min-1. The potential of the system was investigated for the determination of various iron species in synthetic samples containing iron(II) and iron(III) in ionic or chelated form by employing various ion-exchange (Dowex 50-X8, Dowex 1-X8) and sorptive (Amberlite XAD-2) resins, respectively. In some instances where direct coupling was impossible, owing to the physical properties of the effluent or eluent, conventional analyses of chromatographically separated iron species were performed by flame AAS. The optimum concentration range, limit of detection and reproducibility of measurement were also determined for a particular column capacity. When direct coupling was employed, the detection limit for the separated iron species was 15 micrograms with a relative standard deviation (RSD) of +/- 3% and, using the conventional method of analysis, 2-5 micrograms with an RSD of +/- 1%. On the basis of these results the system was applied to the determination of the ratio of iron(II) to iron(III) in wines.

Chemical forms of manganese in the leaves of manganese hyperaccumulator Phytolacca acinosa Roxb. (Phytolaccaceae)

“…A useful approach to overcome such difficulties is to couple various chromatographic and optical spectrometric methods (Ajlec and Stupar 1989). In the present study, gel filtration analysis showed that oxalic acid and Mn appeared in the same fraction of the supernatant with the ratio of oxalic acid to Mn of 1.12, suggesting that there were sufficient amounts of oxalic acid to chelate Mn.…”

Fluorescence quenchometric method for determination of ferric ion using boron-doped carbon dots

“…Therefore, accurate and fast determination of Fe(III) is an important issue in the environment and in biological systems. Up to now, numerous methods have been developed for the detection of Fe(III), including flame atomic absorption spectroscopy [14], mass spectrometry [15], electrochemical [16], and fluorescence detection [17][18][19][20][21][22][23][24][25][26]. Among these methods, fluorescence detection is a favorable method due to their high sensitivity, selectivity and fast detection.…”

Conjugated polymer nanoparticles with aggregation induced emission characteristics for intracellular Fe3+ sensing

“…2,3 Tremendous efforts have been made to develop sensitive Fe 31 monitoring approaches, including UV-Vis spectrophotometry, 4 inductively coupled plasma mass spectrometry, 5 flame atomic absorption spectrometry, 6 voltammetry, 7 fluorescence spectrophotometry. [8][9][10] Among these approaches, fluorescence spectroscopy is particularly promising owing to its simplicity, high sensitivity, and ability to facilitate real-time detection.…”