Determination of inorganic fiber density in human lung tissue by scanning electron microscopy after low temperature ashing.

Inorganic fibers in lung tissue from patients with pleural plaques or malignant mesothelioma.

“…Ol"gank material was removed by lowtemperature plasma ashing. The ash was analyZled ac-cordJing to the methods descl"i,bed by Gylseth et al (4,5). No effort was made to identify all fi.…”

Analysis of titanium pigments in human lung tissue

“…Scanning electron microscopy and Xray microanalysis were performed on dehydrated and critical-point dried lung tissue, as well as on low-temperature ashed samples prepared according to the method described by Gylseth et al (4). All samples were mounted on carbon stubs with carbon cement and coated with gold to make them conductive.…”

Analysis of inorganic fiber concentrations in biological samples by scanning electron microscopy.

“…For lower fiber 10ading r s the precision was even bet/trer fo,rtlhe Nuclepor,e membranes. The RSD for the tissue samples was better than 20 010, includimg intra-and interlobar fi/ber density variation (4). A rrelationship between the magnificaltion ,employed and fiber numbers has previously been demonstrated by Gylseth et a:l (4).…”

“…Compared to tlle structure of plain Nudepore membranes, the porous structure of the membranes is a poor ba~kground for SEM work, hut penetra1ion of the Nuclepore may occur depending on the pore sire (4,8).…”

“…•_----.~------low-temperature ashing of the tissue and ultrasonic dispersion of the ash are contramdicated. Such teclmiques have, on the o1fuer hand, previlously -been used by other authors (3,4,7). Different preparation techniques have, however, not been thoroughly compared.…”