Determination of inorganic anions in saliva by capillary isotachophoresis

Sádecká, Jana; Polonský, J.

doi:10.1016/s0039-9140(02)00467-8

Cited by 25 publications

(19 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Amperometric [47], conductivity [13,[28][29][30] or potentiometric detectors [42,44,49,53] were used as well. Important information on the behavior of iodide in various electrolyte systems can be obtained also from isotachophoretic analyses where the effects of PVP [54,55], quaternary ammonium salts and surfactants [56], cyclodextrins [57,58], and Cd (II) [59] were studied. An additional source of information is a series of reviews focused on ITP [60,61] or CZE separation of inorganic ions [62][63][64][65][66][67][68][69][70][71][72][73][74][75][76][77][78].…”

Section: Ce and Cecmentioning

confidence: 99%

Fast and simple method for determination of iodide in human urine, serum, sea water, and cooking salt by capillary zone electrophoresis

Pantůčková

Křivánková

2004

Electrophoresis

View full text Add to dashboard Cite

For the determination of iodide in urine, where 80-90% of consumed iodine is excreted, a fast, simple, and sensitive method of capillary zone electrophoresis was elaborated and tested also for additional complex matrices such as human serum, cooking salt, and seawater. Several approaches were examined for the separation of iodide from other macro- and microcomponents in the tested matrices, and the best results were obtained when host-guest interaction with alpha-cyclodextrin or ion-pairing with polyethylenimine was employed. In both cases comparable resolution and sensitivity were reached. Due to the relatively high price of cyclodextrin only the method with polyethylenimine was further optimized and a simple procedure enabling the determination of iodide in untreated human urine, serum, cooking salt, and seawater was elaborated. The samples were injected for 20 s at 0.5 psi (3.45 kPa) into a fused-silica capillary (0.18 mm ID, 50 cm effective length) coated with polyacrylamide (electroosmotic flow < 2 x 10(-9) m(2)V(-1)s(-1)) and filled with the optimized background electrolyte composed of 20 mM KH(2)PO(4) and 0.7% m/v polyethylenimine. For detection, UV absorption at 200 and 230 nm was measured. Concentration limits of detection reached at 230 nm were for human urine 0.14 microM, for human serum 0.17 microM, for seawater 0.17 microM, and for cooking salt 89 nM. Relative standard deviations of iodide peak area and height in all matrices ranged within 0.93 to 4.19%.

show abstract

Section: Ce and Cecmentioning

confidence: 99%

Fast and simple method for determination of iodide in human urine, serum, sea water, and cooking salt by capillary zone electrophoresis

Pantůčková

Křivánková

2004

Electrophoresis

View full text Add to dashboard Cite

show abstract

“…The operating conditions were selected to prevent both adsorption of the analyte to the capillary wall and formation of micelles (LE: 5 mM potassium acetate, pH 4.5, 0.2% HEC, 30% ethanol v/v; TE: 5 mM b-alanine 1 acetic acid, pH 4.0). Nitrite, nitrate, iodide, and thiocyanate were quantified in nonsmoker and smoker saliva by ITP within a large linearity range between 0.005 and 0.500 mM (LE: 10 mM HCl 1 histidine, 6% PVP, pH 6.0; TE: 5 mM acetic acid) [81]. ITP with conductivity detection was used to determine acetic, lactic, propionic, and butyric acids in silages with LOQs on the 5-10 mM level (LE: 10 mM HCl 1 22 mM EACA, 0.05% HPMC, pH 4.5; TE: 5 mM caproic acid) [82].…”

Section: Miscellaneousmentioning

confidence: 99%

Recent progress in capillary ITP

2007

View full text Add to dashboard Cite

ITP has been attracting constant attention for many years due to its principal capability to concentrate trace analytes by several orders of magnitude. In the current capillary format, it is able to concentrate trace analytes diluted to several microliters of an original sample into concentrated zones having volumes in the range of picoliters. Due to this reason, ITP holds an important position in many current multistage and multidimensional separation schemes. This article links up previous reviews on the topic and summarizes the progress of analytical capillary ITP since 2002. Almost 100 papers are reviewed that include methodological novelties, instrumental aspects, and analytical applications. Papers using ITP and/or isotachophoretic principles as part of multistage and/or multidimensional separation schemes are also included.

show abstract

“…For example, determination of the sodium and chloride concentrations in sweat may confirm the diagnosis of cystic fibrosis, a life-threatening, autosomal recessive disease (LeGrys 1996(LeGrys , 2007. The level of thiocyanates (cyanide metabolites) in saliva and urine is considered a good test for distinguishing between smokers and non-smokers, and its determination is useful for evaluating smoking behaviour (Bilewicz and Kublik 1981;Giraudi and Grillo 1981;Chinaka 1998;Glatz et al, 2001;Connolly et al, 2002;Themelis and Tzanavaras 2002;Sa´decka´and Polonsky´2003;Chen et al, 2004a, b;Tanaka 2004;Demkowska et al, 2008;Tamosˇiu´nas et al, 2006). The subject literature still lacks information on the determination of these ions in other biological fluids, such as semen and sweat.…”

Section: Introductionmentioning

confidence: 99%

Non-invasive biological fluid matrices analysed to assess exposure to environmental tobacco smoke

Demkowska

Polkowska

Namieśnik

2011

J Expo Sci Environ Epidemiol

View full text Add to dashboard Cite

Human biomonitoring (analysis of biological fluids) is increasingly being used for assessing exposure to environmental pollutants. Smoking tobacco is a significant source of indoor air pollution and is harmful to human health. The aim of this research was to find both the best non-invasive matrices (from among saliva, urine, semen and sweat) for evaluating environmental exposure to tobacco smoke and the relationships between thiocyanates (biomarkers of environmental tobacco smoke exposure) and other inorganic ions in these matrices collected from active and passive smokers and also non-smokers.

show abstract

Determination of inorganic anions in saliva by capillary isotachophoresis

Cited by 25 publications

References 19 publications

Fast and simple method for determination of iodide in human urine, serum, sea water, and cooking salt by capillary zone electrophoresis

Fast and simple method for determination of iodide in human urine, serum, sea water, and cooking salt by capillary zone electrophoresis

Recent progress in capillary ITP

Non-invasive biological fluid matrices analysed to assess exposure to environmental tobacco smoke

Contact Info

Product

Resources

About