Determination of indican and tryptophan in normal and uraemic patients by high-performance liquid chromatography with a new electrochemical detector

Laganà, Aldo; Liberti, A.; Morgia, C.; Tarola, Anna Maria

doi:10.1016/s0378-4347(00)80702-1

Cited by 12 publications

(6 citation statements)

References 12 publications

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“…Plasma samples were deproteinized by addition of 900 lL of methanol to 300 lL of plasma. This method which is derived from that of Lagana et al [10] provided indican recovery of 102 ± 2% and phenol red recovery of 108 ± 7% (four runs at 1.0 mg/dL and 1.5 mg/dL, respectively). Samples of the resulting supernatant were assayed by fluorescence detection (excitation 250 nm, emission 410 nm) following processing on a C18 column using gradients of 50 mmol/L ammonium formate and methanol as described by Lesaffer et al [11].…”

Section: Clearance Measurements During Vitro Cvvh and Cvvhdfmentioning

confidence: 99%

The clearance of protein-bound solutes by hemofiltration and hemodiafiltration

Meyer¹,

Walther²,

Pagtalunan³

et al. 2005

Kidney International

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Section: Clearance Measurements During Vitro Cvvh and Cvvhdfmentioning

confidence: 99%

The clearance of protein-bound solutes by hemofiltration and hemodiafiltration

Meyer¹,

Walther²,

Pagtalunan³

et al. 2005

Kidney International

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“…IS was eluted at 5.5 minutes in an isocratic run. The detector voltage was set at +400 mV ([ 21 ], with modifications). The quadratic calibration curve was prepared in a concentration range of 12–400 μ mol/l and used for quantitation.…”

Section: Methodsmentioning

confidence: 99%

Indoxyl Sulfate Elimination in Renal Replacement Therapy: Influence of Citrate- versus Acetate-Buffering Component during Bicarbonate Dialysis

et al. 2018

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Indoxyl sulfate has been identified as a major factor in the dysregulation of several genes. It is classified as a poorly dialyzable uremic toxin and thus a leading cause in the poor survival rate of dialysis patients. A monocentric, prospective, open cohort study was performed in 43 male patients undergoing chronic renal replacement therapy in a single hemodialysis center. The aim of the study was to determine the influence of acetate- versus citrate-buffered dialysis fluids in hemodialysis (HD) and postdilution hemodiafiltration (HDF) settings on the elimination of indoxyl sulfate. Also, additional factors potentially influencing the serum concentration of indoxyl sulfate were evaluated. For this purpose, the predialysis and postdialysis concentration ratio of indoxyl sulfate and total protein was determined. The difference was of 1.15 (0.61; 2.10), 0.89 (0.53; 1.66), 0.32 (0.07; 0.63), and 0.44 (0.27; 0.77) μmol/g in acetate HD and HDF and citrate HD and HDF, respectively. Acetate HD and HDF were superior when concerning IS elimination when compared to citrate HD and HDF. Moreover, residual diuresis was determined as the only predictor of lower indoxyl sulfate concentration, suggesting that it should be preserved as long as possible. This trial is registered with EU PAS Register of Studies EUPAS23714.

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“…Aliquots of serum were divided into two portions, one of which was used to determine the total concentrations of Trp and IS and the other to measure F u of IS. The samples were prepared as described previously ( Laganà et al, 1986). For determination of the former, 200 l of methanol was added to 100 l of serum.…”

Section: High-performance Liquid Chromatographymentioning

confidence: 99%

In Vivo Kinetics of Indoxyl Sulfate in Humans and Its Renal Interaction with Angiotensin-Converting Enzyme Inhibitor Quinapril in Rats

Fujita¹,

Ishihara²,

Yasuda³

et al. 2012

J Pharmacol Exp Ther

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Indoxyl sulfate (IS) is an organic anion uremic toxin that accumulates in patients with chronic kidney disease (CKD). The aims of this study were to examine the kinetic profiles of IS in humans at a steady state after multiple doses of L-Trp, a precursor of IS, and the in vivo interaction of IS with the angiotensin-converting enzyme inhibitor quinapril, whose active metabolite is a substrate of organic anion transporter 3 (OAT3) in rats. First, 12-h kinetics after single doses of Trp (2, 4, and 8 g) were examined in two healthy volunteers. Second, 24-h kinetics after a single dose of 2 g of Trp was studied in six volunteers. Third, 35-h kinetics after single and multiple doses of 2 g of Trp were examined in five volunteers. In anesthetized rats, quinapril or probenecid, an inhibitor of OATs, was given intravenously before IS, and blood and urine samples were taken until 90 min. Trp and IS concentrations were determined by high-performance liquid chromatography. Ultrafiltration was used to measure serum unbound IS concentrations. Renal tubular secretion of IS accounted for more than 90% of its renal clearance in the steady state of serum IS levels after multiple doses in humans. In animals, the serum area under the curve of IS increased in conjunction with a decrease in renal clearances after coadministration of IS with quinapril or probenecid. It is concluded that quinapril may inhibit the urine excretion of IS via OAT3-mediated renal tubular transport in patients with CKD.

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Determination of indican and tryptophan in normal and uraemic patients by high-performance liquid chromatography with a new electrochemical detector

Cited by 12 publications

References 12 publications

The clearance of protein-bound solutes by hemofiltration and hemodiafiltration

The clearance of protein-bound solutes by hemofiltration and hemodiafiltration

Indoxyl Sulfate Elimination in Renal Replacement Therapy: Influence of Citrate- versus Acetate-Buffering Component during Bicarbonate Dialysis

In Vivo Kinetics of Indoxyl Sulfate in Humans and Its Renal Interaction with Angiotensin-Converting Enzyme Inhibitor Quinapril in Rats

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