Determination of In Vitro Cytotoxicity and Anti-Angiogenesis for a Bioactive Compound from Aspergillus terreus FC36AY1 Isolated from Aegle marmelos around Western Ghats, India

Abstract

The biotechnological research mainly emphasis its investigation on searching new natural drugs at economical to human welfare. With this view in mind, this research has focused to develop a prospective bioactive compound isolating from an efficient endophytic fungus isolated from potential medicinal tree Aegle marmelos. The endophytic fungus was isolated from the medicinal tree and identified as Aspergillus terreus FC36AY1. This fungus produced maximum of crude metabolites and this was produced in Sabouraud's Dextrose Broth. The produced metabolites were extracted using acetone as a sole solvent and it was taken for the assessment of antimicrobial and antioxidant analysis. The crude metabolites exhibited maximum activity at least concentration and further the crude extract were taken for purification processes through chromatographic techniques. Through purification, five different fractions were eluted and those five different fractions were also assessed for antimicrobial and antioxidant analysis. From these analysis results, TA4 was found to be efficient fraction and it was characterized through FT-IR, GC-MS and UV-VIS analysis. The compound was taken for cytotoxicity determination in HT-29 cancer cells and anti-angiogenesis analysis was assessed through HET-CAM testing. The bio-activities study revealed that the compound TA4 has the ability to target the cancer cells in an efficient manner.