Determination of in vitro antidiabetic effects of Zingiber officinale Roscoe

Abstract

Aqueous extracts of Zingiber officinale rhizomes were studied to evaluate their antidiabetic effects on protein glycation and on the diffusion of glucose in vitro in the present study. Zingiber officinale rhizome aqueous extract were examined at concentrations of 5, 10, 20 and 40 g/L. The antidiabetic effects were found to be dose-dependent. Antidiabetic potential of Zingiber officinale was mainly through inhibition of the glucose diffusion and to a limited extent by reducing the glycation. However, further studies are needed to determine in vitro effects of therapeutic potential by restraining postprandial glucose absorptions and plasma protein glycations in diabetic subjects.Uniterms: Zingiber officinale/pharmacognosy. Zingiber officinale/antidiabetic effects/in vitro study. Diabetes mellitus. Hyperglycemia. Proteins/glycation. Glucose/diffusion in vitro.Extratos aquosos de rizomas Zingiber officinale foram estudados para avaliar os seus efeitos antidiabéticos em glicação de proteínas e sobre a difusão de glicose in vitro, no presente estudo. Extratos aquosos de Zingiber officinale foram examinados nas concentrações de 5, 10, 20 e 40 g extrato de planta/L. Os efeitos antidiabéticos observados eram dependentes da dose. O potencial antidiabético de Zingiber officinale se verificou, principalmente, através da inibição da difusão de glicose e, em menor extensão, através da redução da glicação. Estudos adicionais são necessários para elucidar se efeitos in vitro representam potencial terapêutico, restringindo a absorção de glicose pós-prandial e a glicação de proteínas plasmáticas em indivíduos diabéticos. Unitermos:Zingiber officinale/farmacognosia. Zingiber officinale/efeitos antidiabéticos/estudo in vitro. Diabetes mellitus. Hiperglicemia. Proteinas/glicação. Glicose/difusão in vitro.

In vitro comparative assessment of the inhibitory effects of single and combined spices against glucose-synthesizing enzymes

“…Alteration in diet composition and lifestyle is therefore vital in its prevention and management. The use of spices in diet is being acknowledged as a means of managing DM since diets rich in spices have been found to be abundant in medicinal compounds [12].…”

Pharmacological Potentials of Ginger

“…A similar example is the report of Ojewole [48] as submitted previously in a section (above) of this report. Similarly, 500 mg/kg bw of its aqueous extract lowers plasma sugar level following streptozotocin induction [50] in animal model and in vitro [51]. Since there is a report of correlation between oxidative stress and DM [20] as well as other complications of DM such as hyperlipidemia, hypercholesterolemia, retinopathy, and neuropathy, various publications had revealed the potentials of ginger and its derivatives against these complications as cited by Gunathilake and Rupasinghe [34].…”

A Review of the Antidiabetic Activities of Ginger

“…According to Dearlove et al [57], spices such as cinnamon, cloves, oregano, and allspice possess bioactive compounds that have (1) antiglycation properties which inhibit the formation of AGEs; (2) Rani et al, [44] Aqueous extract of ginger at 5, 10, 20, 40 g/L incubated with (PBS), glucose + BSA for 5 weeks Dose-dependent, antidiabetic activity through inhibition of glucose diffusion and reduced glycation Sattar et al, [45] effects of ROS; and (3) anti-inflammatory potentials. Some studies associate the antidiabetic action of ginger to its bioactive principles such as gingerol and shogaol which have the capacity to enhance glucose uptake in rat's skeletal muscle cells, and promote increased expression and translocation of GLUT-4 glucose transporter to the plasma membrane of the cells thus clearing excess glucose from the serum [34].…”

“…Some studies associate the antidiabetic action of ginger to its bioactive principles such as gingerol and shogaol which have the capacity to enhance glucose uptake in rat's skeletal muscle cells, and promote increased expression and translocation of GLUT-4 glucose transporter to the plasma membrane of the cells thus clearing excess glucose from the serum [34]. Another mechanism proposed was the inhibition of key enzymes of carbohydrate metabolism-α-glucosidase and α-amylase by phenolic compounds (gingerols and shogaols) present in ginger [23,45]; while other authors showed that ginger increases muscle and liver glycogen stores by enhancing peripheral utilization of glucose, thus limiting gluconeogenesis in the liver and kidney in a manner similar to insulin [37].…”