Determination of Imazaquin and its Metabolite by Liquid Chromatography-Quadrupole-Time of Flight Tandem Mass

Abstract

A method consisting of solvent extraction followed by liquid chromatography-quadrupole-time of flight- tandem mass spectrometry analysis was developed for the identification of Imazaquin and its metabolite. The relationships between detector response and sample concentrations showed a high degree of linearity (r > 0.998) over the range 0.03-10 microg/g. The recoveries obtained were in the acceptable range of 86%-104% between spiked. The relative standard deviation of this method was 6.4%-17.1%. A 35-day study of Imazaquin degradation was taken in agricultural soil from Binzhou, China. The degradation followed first order kinetics (C = 0.7672e(-0.0774t)), with half-life of less than 8.5 days. Investigation of the by-products from liquid chromatography-quadrupole-time of flight- tandem mass spectrometry has shown that there were four important metabolites 4-methylene-2-(quinolin-2-yl)-1H-imidazol-5(4H)-one, quinoline-3-carbaldehyde, 1-amino-2,3-dimethyl-1-oxobutan-2-ylium and 1H-[1,2]oxazino[4,5-b]quinolin-1-one in the degradation process. The accurate mass measurements error was 5 ppm in this study. The method was successfully applied to the analysis of imazaquin and its metabolite residues in soil.

Current use of high-resolution mass spectrometry in the environmental sciences

“…LC-(Q)TOF MS has been used to study the photodegradation of triazine herbicides in water [50], pesticides belonging to different chemical classes [74], bentazone [75] and fenhexamid [76], in water, and imazaquin in soil [77], among others. Recent studies have also been focused on identification of pharmaceuticals and drugs of abuse, because of increasing concern of potential negative effects in the environment.…”