Determination of IgG subclasses and Gm allotypes in culture supernatants of pokeweed mitogen-stimulated human blood lymphocytes.

Morell, A.; Loghem, Erna van; Nef, M; Theilkaes, L.; Skvaril, F

doi:10.4049/jimmunol.127.3.1099

The Journal of Immunology

1981

DOI: 10.4049/jimmunol.127.3.1099

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Determination of IgG subclasses and Gm allotypes in culture supernatants of pokeweed mitogen-stimulated human blood lymphocytes.

A. Morell¹,

Erna van Loghem²,

M Nef³

et al.

Abstract: Concentrations of the 4 IgG subclasses, of IgM, and of IgA were determined in culture supernatants of pokeweed mitogen-stimulated mononuclear cells isolated from human peripheral blood. Time-course studies showed cumulative secretion of IgM, IgA, and the subclasses IgG1, IgG2, and IgG3 but not of IgG4. In supernatants of 7-day cultures of unseparated mononuclear cells, IgG1 was predominant, followed by IgG2 and IgG3. Removal of most monocytes from the mononuclear cells resulted in an overall decrease of immuno… Show more

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Cited by 16 publications

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Determination of ectoenzyme activities in leukemic cells and in established hematopoietic cell lines

et al. 1986

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Ectoenzyme activities were determined in peripheral blood cells from patients with acute leukemias, from normal controls, and in cells of hematopoietic cell lines. In common acute lymphoblastic leukemia, cell membrane-associated 5'-nucleotidase (5'-N) activity was significantly higher than in acute T and unclassified lymphoblastic leukemias. In acute myeloblastic and myelomonocytic leukemias, cells contained significantly higher gamma-glutamyl transpeptidase (gamma-GT) activity than in lymphoblastic leukemias. Normal B lymphocytes differed from T cells and monocytes mainly in their 5'-N activity, whereas in monocytes, gamma-GT activity was more pronounced than in other normal blood cells. Hematopoietic cell lines showed some distinct patterns of ectoenzyme activity. Most B cell lines had high 5'-N and (Na-K-Mg) adenosine triphosphatase activities. In lines of myeloid origin, elevated gamma-GT values were found. In lymphoid stem cells and in T lymphoblast lines, most ectoenzyme activities were lower than in the other cell lines. In some cell lines, characteristic high-activity marker enzymes were detected.

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Determination of ectoenzyme activities in leukemic cells and in established hematopoietic cell lines

et al. 1986

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Ontogeny of the humoral response to group A streptococcal carbohydrate: Class and IgG subclass composition of antibodies in children

Morell

Skvaril

1990

Eur J Immunol

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We determined isotypes of natural antibodies to streptococcal group A carbohydrate (A-CHO) in sera from 101 children between 1 and 16 years of age, using a calibrated enzyme-linked immunosorbent assay system. Anti-A-CHO IgM could be detected in all but one sera. Median levels increased with age and were highest between 8 and 12 years. IgG antibodies were present at low concentrations up to the age of 4 years, and consisted predominantly of the IgG1 subclass. Between 4 and 8 years, concentrations of anti-A-CHO IgG markedly increased and median levels continued to increase through age 12-16. Anti-A-CHO IgG1 levels closely followed the pattern of IgG antibody concentrations. The number of IgG2 antibody positive sera was low in young children, as expected. In the 8-12 year age group and later, anti-A-CHO IgG2 was present in more than half of the samples, and in children between 12 and 16, medians of IgG2 and IgG1 antibodies were similar. Sera containing anti-A-CHO IgG3 were rare in children up to 4 years of age, but in the group of 4-8-year-old children, this subclass was detectable in 36% and later in up to 77% of the sera. Thus, the IgG response to A-CHO showed a clear maturation during childhood, involving the subclasses IgG1, IgG2 and IgG3. There were no significant differences in A-CHO levels between boys and girls.

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T-cell regulation of IgG subclass expression by mitogen-induced plasma cells: Soluble factors versus the T cells

1984

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The human IgG subclasses expressed by plasma cells generated from circulating B cells in response to soluble T-cell factors were examined by immunofluorescence using subclass-specific monoclonal antibodies. Soluble T-cell factors were induced by a mixed lymphocyte reaction, pokeweed mitogen (PWM), or phytohemagglutinin. The distribution of IgG subclasses expressed by plasma cells induced by these factors was IgG2 greater than 75%, IgG1 less than 25%, IgG3 less than 1%, and IgG4 less than 1%. On the other hand, IgG1 was dominant when B cells were cultured with T cells and PWM: IgG1 approximately 70%, IgG2 approximately 20%, IgG3 approximately 8%, and IgG4 approximately 1%. The addition of different amounts of the T-cell factors to B cells in culture did not alter the predominance of IgG2 plasma-cell differentiation. These results suggest that T cells and their soluble factors may preferentially enhance terminal differentiation of different IgG B-cell subpopulations. In contrast, the ratio of IgA1 to IgA2 plasma-cell responses was approximately 1.5 to 1 regardless of whether the B-cell precursors were induced by T-cell factors or by the T cells plus PWM.

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Determination of IgG subclasses and Gm allotypes in culture supernatants of pokeweed mitogen-stimulated human blood lymphocytes.

Cited by 16 publications

References 0 publications

Determination of ectoenzyme activities in leukemic cells and in established hematopoietic cell lines

Determination of ectoenzyme activities in leukemic cells and in established hematopoietic cell lines

Ontogeny of the humoral response to group A streptococcal carbohydrate: Class and IgG subclass composition of antibodies in children

T-cell regulation of IgG subclass expression by mitogen-induced plasma cells: Soluble factors versus the T cells

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