Determination ofAconitumAlkaloids in the Tubers ofAconitum japonicumusing Gas Chromatography/Selected Ion Monitoring

Abstract

A rapid, specific and precise method using GC/SIM was applied to separate and quantify Aconitum alkaloids in Aconitum tubers. The TMS derivatives of each alkaloid produced a well defined peak on selected ion recording (SIR). Good linear response over the range of 100 pg - 7.5 ng was demonstrated for each alkaloid. Furthermore, we investigated the changes in the contents of Aconitum alkaloids, i.e., hypaconitine, mesaconitine, aconitine, and jesaconitine, in Aconitum tubers paying special attention to the harvesting season. Mesaconitine had the highest value in all seasons. Mesaconitine and aconitine showed the highest value in the samples harvested in May 1991 while hypaconitine had the highest value among those harvested in December 1990. As to the total amount of the alkaloids, the highest value, 4190 microg/g, and the lowest value, 1881 microg/g, were observed in the samples harvested in May and September, respectively. GC/SIM was very useful for the determination of Aconitum alkaloids in the tubers.

Effects of long-term administrations of aconitine on electrocardiogram and tissue concentrations of aconitine and its metabolites in mice

“…Aconitine, mesaconitine, hypaconitine, and jesaconitine are the major toxic Aconitum alkaloids. Although the raw root is also highly toxic [2], it has been used for centuries in Oriental Medicine. Sometimes fatal accidents have been reported due to the consumption of the raw root [3].…”

CE‐electrochemiluminescence with ionic liquid for the facile separation and determination of diester‐diterpenoid aconitum alkaloids in traditional Chinese herbal medicine

“…To utilize this herbal medicine safely, it is urgent to develop a simple, fast and efficient method for simultaneous determination of these three alkaloids. Among the analytical techniques, GC/MS [7,8], HPLC [9,10] and CE [11,12], have been used for the determination of these three alkaloids. However, CE is getting more frequently used owing to its inherent high separation efficiency and simplicity of instruments.…”

Determination of aconitine, hypaconitine and mesaconitine in urine using hollow fiber liquid-phase microextraction combined with high-performance liquid chromatography

“…Several methods have been reported for the determination of AC, HA and MA in herbal plants, medicines and body fluid, including CE [9,10], HPLC [11][12][13], LC-MS [2,14,15], LC-MS-MS [3,16,17], GC-MS [1,18], and MS [19,20]. Most of these methods need complex sample pretreatments, such as liquid-phase extraction, solid phase extraction and pre-column derivation, which are time-consuming, tedious, not sensitive enough for trace analysis of aconitines in biofluid, and need large amounts of toxic organic solvents.…”