Determination of Hyoscyamine and Scopolamine in Datura innoxia Plants by High Performance Liquid Chromatography

Plank, Karin-Heide; Wagner, Karl

doi:10.1515/znc-1986-0404

Cited by 27 publications

(5 citation statements)

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“…Thus, the extract might affect the thrombopoiesis and produce harmful effects. These results agree with previous studies reporting that D. innoxia contains tropane alkaloids [33,40] which have toxic effects on biochemical and haematological parameters [9]. Some of these aspects could also have an increased relevance in the context of the potential therapeutical value of some plant extracts in the current pathologies and their related complications [22,36].…”

Section: Table IIsupporting

confidence: 92%

Brain Protection and Anti-Amnesic Effects Assessment of Datura Innoxia Mill. Aqueous Extracts

Kinda¹

2020

FARMACIA

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Traditional plants are largely used in alternative medicine for many disorders treatment. However, some discussions regarding the safety of their usage exist. The present study aims to evaluate the anti-amnesic effect of Datura innoxia leaves aqueous extract, and the possible noxious impact of its use. Thus, there were evaluated the possible protective effects of the extract on the central nervous system, as well as its toxic effects, by using Wistar rats models and NMRI mice, respectively. The results are showing non-protective effects of the aforementioned extract in the behavioural tests, as well as in the levels of acetylcholinesterase, superoxide dismutase and catalase specific activity evaluation. Superoxide dismutase and catalase activity also suffered some significant decreases in the extract-treated rats. In addition, the toxicity results showed no mortality in acute and subacute evaluation. However, behavioural disturbance in Y maze and elevated plus maze tests were observed in all animals receiving acute toxicity doses (2400 and 3000 mg/kg b.w.). Likewise, for the subacute study, there was a body weight lost, biochemical and haematological changes in the extract treated groups. In conclusion, this study suggests that D. innoxia leaves aqueous extracts do not show any anti-amnesic effect on scopolamine-induced memory impairment rats. It could be considered low toxic up to the dose level of 3000 mg/kg b.w. in oral acute use and at the dose level up to 400 mg/kg b.w. in oral subacute use. These results could be of interest for the further use of this extract in traditional or clinical medicine. Rezumat Plantele tradiționale sunt utilizate foarte frecvent în medicina alternativă pentru tratamentul multor patologii. Cu toate acestea, există o serie de discuții cu privire la siguranța utilizării acestora. Prezentul studiu își propune să evalueze efectul anti-amnezic al extractului apos de frunze de Datura innoxia și posibilul impact toxic al utilizării sale. Astfel, au fost evaluate posibilele efecte protectoare ale extractului asupra sistemului nervos central, precum și efectele sale toxice, folosind modele de șobolani Wistar și respectiv șoareci NMRI. Rezultatele au demonstrat absența efectului protector al extractului în testele de comportament, precum și asupra nivelurilor de acetil colinesterază, superoxid-dismutază și catalază. Activitatea superoxiddismutazei și catalazei au prezentat scăderi semnificative la șobolanii tratați. În plus, rezultatele testelor de toxicitate nu au demonstrat mortalitate în evaluare acută și subacută. Cu toate acestea, au fost observate tulburări de comportament în testul labirintului la toate animalele care au primit doze toxice (2400 și 3000 mg/kg corp). De asemenea, pentru studiul subacut, s-a înregistrat o pierdere în greutatea corporală, precum și modificări biochimice și hematologice în grupele tratate cu extract. În concluzie, acest studiu sugerează că extractul apos de frunze de D. innoxia nu are efect anti-amnezic asupra șobolanilor cu deficiențe de memorie induse ...

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Section: Table IIsupporting

confidence: 92%

Brain Protection and Anti-Amnesic Effects Assessment of Datura Innoxia Mill. Aqueous Extracts

Kinda¹

2020

FARMACIA

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“…For the determination of hyoscine-N-butyl-bromide, the method developed by Plank et al [22] and slightly modified by us was used. A Lichrospher RP-C18 column (2504 mm, 5 mm) (Tecnokroma, S.…”

Section: Methodsmentioning

confidence: 99%

Stability and compatibility of binary mixtures of morphine hydrochloride with hyoscine-n-butyl bromide

Barcia

Reyes

Azuara

et al. 2004

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The aim of this study was to determine the compatibility and stability of morphine hydrochloride and hyoscine-N-butyl bromide combined in solution at three different concentrations and stored in polypropylene syringes at 4 degrees C and 25 degrees C over a period of 15 days. The doses assayed were 20, 60 and 120 mg/day for morphine hydrochloride and 40, 60 and 80 mg/day for hyoscine-N-butyl bromide. These dose ranges were chosen according to daily practice. At both temperatures, all mixtures can be considered as physically compatible since no evidence of incompatibility-that is precipitation, turbidity, colour change or opacity and gas production-were observed. After 15 days of storage, the percentages of hyoscine-N-butyl bromide remaining in the drug mixtures tested ranged between 96.07% and 92.23%. At the end of the study, the percentages of morphine hydrochloride remaining in the drug mixtures were 100% at both temperatures.

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“…Indeed, the lone yucca quid in our samples questions what constitutes the remaining in situ quids. Likewise, it may be that other analytical methods may be employed to identify alkaloids in the samples ( 58 , 59 ). Importantly, the dating indicates that Datura use within the cave occurred over several generations, spanning the Late Prehistoric Period into the Historical Period.…”

Section: Discussionmentioning

confidence: 99%

Datura quids at Pinwheel Cave, California, provide unambiguous confirmation of the ingestion of hallucinogens at a rock art site

Robinson

Brown

McMenemy

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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While debates have raged over the relationship between trance and rock art, unambiguous evidence of the consumption of hallucinogens has not been reported from any rock art site in the world. A painting possibly representing the flowers of Datura on the ceiling of a Californian rock art site called Pinwheel Cave was discovered alongside fibrous quids in the same ceiling. Even though Native Californians are historically documented to have used Datura to enter trance states, little evidence exists to associate it with rock art. A multianalytical approach to the rock art, the quids, and the archaeological context of this site was undertaken. Liquid chromatography−mass spectrometry (LC-MS) results found hallucinogenic alkaloids scopolamine and atropine in the quids, while scanning electron microscope analysis confirms most to be Datura wrightii. Three-dimensional (3D) analyses of the quids indicate the quids were likely masticated and thus consumed in the cave under the paintings. Archaeological evidence and chronological dating shows the site was well utilized as a temporary residence for a range of activities from Late Prehistory through Colonial Periods. This indicates that Datura was ingested in the cave and that the rock painting represents the plant itself, serving to codify communal rituals involving this powerful entheogen. These results confirm the use of hallucinogens at a rock art site while calling into question previous assumptions concerning trance and rock art imagery.

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Determination of Hyoscyamine and Scopolamine in Datura innoxia Plants by High Performance Liquid Chromatography

Cited by 27 publications

References 13 publications

Brain Protection and Anti-Amnesic Effects Assessment of Datura Innoxia Mill. Aqueous Extracts

Brain Protection and Anti-Amnesic Effects Assessment of Datura Innoxia Mill. Aqueous Extracts

Stability and compatibility of binary mixtures of morphine hydrochloride with hyoscine-n-butyl bromide

Datura quids at Pinwheel Cave, California, provide unambiguous confirmation of the ingestion of hallucinogens at a rock art site

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