Determination of hydroxylated fatty acids from the biopolymer of tomato cutin and their fate during incubation in soil

Abstract

We produced evidence that the HCl treatment only affected free hydroxyl groups and thus could be used for proportioning free and bound OH-groups on cutin fatty acids. The method enabled a direct quantification of the ω-hydroxy fatty acids throughout the incubation phase.

Reconstitution of cutin monomers on smectite surfaces: adsorption and esterification

“…The final cutin monomer isolate was composed mainly by diHPA (80%) whereas coumaric acid, hydroxyhexadecanoic acid and triHPA made up 7, 3.5 and 3%, respectively. These data are consistent with previous studies (Gérard and Osman, 2007;Hauff et al, 2010;Kolattukudy, 1980;Riley and Kolattukudy, 1975). Aqueous solutions of the monomers were prepared by evaporating the ethyl acetate and re-dissolving the materials in double-distilled water.…”

“…The concentrations of diHPA and triHPA were measured by GC, following silylation of the fatty acids (Hauff et al, 2010). Briefly, aliquots of supernatants were dried under stream of N 2 and re-dissolved in a mixture of pyridine and BSTFA + TMCS (25/75, v/v) at 70°C for 30 min.…”

Towards green chemicals and edible coatings from barks and peels with near critical extraction of suberin

“…An analogous application has already been successfully implemented with cutin extracts. 49 The suberinic extract obtained under milder depolymerizing conditions was sprayed as an ethanolic solution at two concentrations: 10 and 50 mg mL −1 . The 50 mg mL −1 concentration was chosen as it approached the solubility limit of the extract in ethanol at room temperature.…”

Processing, Valorization and Application of Bio-Waste Derived Compounds from Potato, Tomato, Olive and Cereals: A Review

“…In some cases, an additional pretreatment of tomato skins is performed, which consists of a heat treatment with oxalic acid and ammonium oxalate to remove the residual pulp, that can remain attached to the skins [257]. Previous methods which successfully conducted organic solvent-free cutin extractions are very scarcely, in particular using tomato by-products as raw material.…”