Determination of ?-hydroxybutyric acid (GHB) in plasma and urine by headspace solid-phase microextraction and gas chromatography/positive ion chemical ionization mass spectrometry

Frison, Giampietro; Tedeschi, L.; Maietti, Sergio; Ferrara, Santo Davide

doi:10.1002/1097-0231(20001230)14:24<2401::aid-rcm179>3.0.co;2-i

Cited by 51 publications

(19 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Qualitative and quantitative determination of GHB and GBL in various matrices has been carried out employing GC-PICI-MS, often using methane as CI reagent [12]. In many published methods, the procedure takes advantage of the quantitative conversion of GHB into GBL by dehydration;…”

Section: Page 6 Of 20mentioning

confidence: 99%

A methodological approach to the selection of liquid reagents for chemical ionization ion trap-gas chromatography mass spectrometry: A case study of GBL and 1,4-BD

Guandalini¹,

Soldani²,

Rosi³

et al. 2015

International Journal of Mass Spectrometry

View full text Add to dashboard Cite

Section: Page 6 Of 20mentioning

confidence: 99%

A methodological approach to the selection of liquid reagents for chemical ionization ion trap-gas chromatography mass spectrometry: A case study of GBL and 1,4-BD

Guandalini¹,

Soldani²,

Rosi³

et al. 2015

International Journal of Mass Spectrometry

View full text Add to dashboard Cite

“…Since GBL is converted to GHB within minutes after oral ingestion, GHB is the analyte of choice to search for in samples collected from suspected GBL/GHB-intoxicated patients [17,29,30].…”

Section: Selectivitymentioning

confidence: 99%

“…Several options have been described that allow to reduce the work-load associated with GC-MS-based GHB determination in biological fluids. These include the addition of excess derivatization reagents directly to biological samples, thus omitting the extraction step [12][13][14][15], the use of HS injection after conversion of GHB to gamma-butyrolactone (GBL) [16][17][18] or "in-vial" derivatization with for example hexylchloroformate prior to solid-phase microextraction (SPME) [19] or HS injection [20]. The available HS-based methods, either 'classical' static HS [16], SPME [17,20], or solidphase dynamic extraction (SPDE) [18], all start from 0.5-1 ml of biofluid (water, urine, plasma, serum or whole blood), providing sufficient sensitivity (LLOQ from 0.1 to 5.0 µg/ml).…”

Section: Introductionmentioning

confidence: 99%

Determination of gamma-hydroxybutyric acid in biofluids using a one-step procedure with “in-vial” derivatization and headspace-trap gas chromatography–mass spectrometry

Ingels¹,

Neels²,

Lambert³

et al. 2013

Journal of Chromatography A

View full text Add to dashboard Cite

A headspace-trap gas chromatography-mass spectrometry (HS-trap GC-MS) method was developed to determine GHB, a low molecular weight compound and drug of abuse, in various biological fluids. Combining this relatively novel and fully automated headspace technique with "in-vial" methylation of GHB allowed for a straightforward approach. One single method could be used for all biofluids (urine, plasma, serum, whole blood or lyzed blood), requiring only 100 µl of sample. Moreover, our approach involves mere addition of all reagents and sample into one vial. Following optimization of headspace conditions and trap settings, validation was performed. Although sample preparation only consists of the addition of salt and derivatization reagents directly to a 100 µl-sample in a HS-vial, adequate method sensitivity and selectivity was obtained. Calibration curves ranged from 5 to 150 µg/ml GHB for urine, from 2 to 150 µg/ml for plasma, and from 3.5 to 200 µg/ml for whole blood. Acceptable precision and accuracy (<13 % bias and imprecision) was seen for all quality controls (QC's) (LLOQ-level, low, medium, high), including for the supplementary serum-and lyzed blood-based QC's, using calibration curves prepared in plasma or whole blood, respectively. Incurred sample reanalysis demonstrated assay reproducibility, while cross-validation with another GC-MS method demonstrated that our method is a valuable alternative for GHB determination in toxicological samples, with the advantage of requiring only 100 µl and minimal hands-on time, as sample preparation is easy and injection automated. KeywordsGamma-hydroxybutyric acid (GHB), in-vial derivatization, gas chromatography coupled to mass spectrometry (GC-MS), headspace-trap (HS-trap), biological matrices, toxicology 3

show abstract

“…Le plus souvent, le GHB est converti en GBL par un acide (22). Une extraction liquide-liquide (par exemple avec du dichlorométhane (23)) ou une micro-extraction en phase solide dans l'espace de tête peut être utilisée (24). Une autre alternative consiste à faire une extraction du GHB puis à procéder à son dosage par CG ou CG-SM après dérivation (25,26).…”

Section: Kétamineunclassified

Apport de l'analyste lors d'intoxications par les nouvelles drogues de synthèse

Verstraete¹,

Deveaux

2000

Ann Toxicol Anal

View full text Add to dashboard Cite

show abstract

Determination of ?-hydroxybutyric acid (GHB) in plasma and urine by headspace solid-phase microextraction and gas chromatography/positive ion chemical ionization mass spectrometry

Cited by 51 publications

References 39 publications

A methodological approach to the selection of liquid reagents for chemical ionization ion trap-gas chromatography mass spectrometry: A case study of GBL and 1,4-BD

A methodological approach to the selection of liquid reagents for chemical ionization ion trap-gas chromatography mass spectrometry: A case study of GBL and 1,4-BD

Determination of gamma-hydroxybutyric acid in biofluids using a one-step procedure with “in-vial” derivatization and headspace-trap gas chromatography–mass spectrometry

Apport de l'analyste lors d'intoxications par les nouvelles drogues de synthèse

Contact Info

Product

Resources

About