Determination of hydrocortisone, polymyxin B and Zn-bacitracin in pharmaceutical preparations by micellar electrokinetic chromatography

Abstract

A new, simple and accurate micellar electrokinetic chromatography (MEKC) method is established for quantification of hydrocortisone, polymyxin B and Zn-bacitracin in local pharmaceutical preparations. The separation was carried out at 25 degrees C and 25 kV, using a 15 mmol L(-1) phosphate-15 mmol L(-1) borate buffer (pH 8.2), 60 mmol L(-1) sodium dodecylsulfate (SDS), and 10% methanol-water (v/v) as background electrolyte. Under these conditions the analysis takes about 23 min. The method has been applied for quantifying these compounds in two different commercial pharmaceutical products and the method gave good results when compared with a reference spectrophotometric multivariate calibration method.

Luminescence methods for study and determination of pollutants in the environment

“…Similarly to the case of PAH CE analysis, the choice of the analytical approach between CE, MEKC, CZE and CEC was made according to the physicochemical properties of pharmaceuticals under study. Therefore, these drugs were generally determined by MEKC or MEEKC methods [208,222,223]. In addition, LIF detection combined with MECK was preferably used, because it was more selective and more sensitive than other luminescence detectors [224][225][226].…”

Direct determination of polymyxin B sulfate using resonance Rayleigh scattering and resonance non‐linear scattering methods with hexatungstate

“…From Table , it can be seen that the RRS, SOS and FDS methods all have a high sensitivity for PMB. The RRS method is the most sensitive, and the detection limit is 5.5 ng/mL, which is higher than those of the reported thin layer chromatography , capillary electrophoresis , high‐performance liquid chromatography‐ultraviolet methods and the RRS method using DNA as a probe (as shown in Table ).…”

“…Several assay methods have been reported for the determination of PMB, such as thin layer chromatography (3), capillary electrophoresis (4)(5)(6) and liquid chromatography (7)(8)(9)(10)(11). The conventional microbiological method requires complicated multiple-step manual operations and a comparatively longer assay time.…”

Simultaneous determination of neomycin sulfate and polymyxin B sulfate by capillary electrophoresis with indirect UV detection

“…Polymyxin B has been determined spectrophotometrically by derivative and multivariate calibration techniques [7,8], by high-performance liquid chromatography (HPLC) [3,5,[9][10][11][12], by thin-layer chromatography (TLC) [13], and also by capillary electrophoresis (CE) [4,[14][15][16][17]. Due to the lack of a chromophore or fluorophore in neomycin molecule, the direct detection by conventionally spectrophotometric method is difficult [1,18].…”