Determination of human urinary kanamycin in one step using urea-enhanced surface plasmon resonance light-scattering of gold nanoparticles

Abstract

The purpose of this study was to establish a simple, sensitive analytical method for kanamycin (KANA) in human urine. Enhancement of the plasmon resonance light-scattering (PRLS) of gold nanoparticles (AuNPs) by KANA provided the basis for this analytical method. At pH 6.7, KANA induced AuNPs aggregation with enhanced PRLS. The PRLS of the AuNPs-KANA system was further enhanced by addition of urea. The linear range and detection limit for KANA were from 20-800 nmol L(-1) and 2 nmol L(-1), respectively. Potential interfering substances present in urine had a negligible effect on the determination, thus preliminary sample separations were not necessary. Recovery of KANA from spiked human urine was 94-104%. This simple, sensitive method, using urea to enhance the PRLS of the AuNPs-KANA system, may provide a new approach for determination of compounds rich in OH groups.

Hollow fiber supported ionic liquid membrane microextraction for preconcentration of kanamycin sulfate with electrochemiluminescence detection

“…In this work, a strong ECL enhancement due to KAM at a glass carbon electrode (GCE) in a Ru(bpy) 3 2+ solution was demonstrated. The sensitivity achieved by this ECL method is very high among the reported methodologies [13][14][15][16][17][18][19][20]. The enhancement mechanism of KAM to the ECL of Ru(bpy) 3 2+ was also discussed.…”

“…Many analytical techniques are available to determine KAM, for example, HPLC-UV [13], immunosensor [14], liquid chromatographic method with pulsed electrochemical detection [15], DNA aptamer sensor [16], cantilever array sensor [17], solid-phase extraction and capillary electrophoresis [18], capillary zone electrophoresis [19], surface plasmon resonance light-scattering [20]. The HPLC and LC methods require expensive apparatus and professional operators.…”

The Resonance Scattering Quantum Yield with Modulated Structure of the Dielectric-Metal Multilayer Nanoshells

“…15 Based on enhancement of the plasmon light scattering of gold nanoparticles, a simple and sensitive analytical method for kanamycin in human urine has been established. 16 It was clearly evident from the theoretical calculation and experiment that the resonance scattering of metallic nanoparticles is highly dependent on the nanoparticle size, shape and structure. [17][18][19] Kuwata et al determined a simple analytical formula to describe the light scattering from the lowest resonance mode of arbitrarily shaped compact metal nanoparticles whose sizes are larger than those accounted for by Rayleigh approximation.…”

A New and Sensitive Catalytic Resonance Scattering Spectral Assay for the Detection of Laccase Activity Using H2O2-I−-TDMAC System

“…[15][16][17][18][19][20] Recently, some immunoreaction, nanogold labeled immunoreaction and immunonanogold catalytic reaction have been utilized to establish some new RS methods for detection of antigen and haptene. [21][22][23][24] In addition, the combining RS technique with inorganic catalytic reaction and enzyme catalytic reaction was applied to analysis of trace inorganic ion, organic compound and enzyme activity.…”