Determination of Homovanillic Acid in Human Plasma Using HPLC with Electrochemical Detection and Automated Solid Phase Extraction

Plasma levels of prolactin (PRL) and the butyrophenone neuroleptic benperidol (BPD) were closely followed 0 to 48 h after acute application of 6 mg BPD as intravenous injection, orally as liquid, and orally as tablets in 12 schizophrenic patients using a partially randomized cross over design. Drug concentrations showed application specific pharmacokinetic behavior with complete elimination within 48 h. All three applications led to a biphasic PRL response with pronounced initial plasma PRL peaks returning to baseline levels within 48 h. The results suggest that after acute neuroleptic challenge BPD plasma levels as low as 2-3 ng/ml can be sufficient for complete depletion of pituitary PRL stores. This initial peak was followed by a PRL plateau about twice above pretreatment values indicating doubling of the PRL synthesis and secretion independent of supraeffective actual BPD concentrations. The PRL plateau persisted as long as BPD concentrations were above those levels which triggered the initial PRL response. As compared with the time of maximum concentrations (tmax) for BPD, the PRL tmax was later after i.v. injection, equal after liquid application, and earlier after tablet administration leading to pronounced application specific differences in shape, direction, and position of resulting hysteresis curves. Plasma levels of homovanillic acid (HVA) were not affected by BPD treatment. The PRL and HVA levels registered after acute doses of BPD indicated that the hormone responses were most likely the result of acute depletion of PRL stores and subsequent stimulation of hormone synthesis whereas it seemed unlikely that dopaminergic activities were relevant.

show abstract

“…Plasma concentrations of HVA were measured by an HPLC method with electrochemical detection previously described (Seiler and Hiemke, 1993).…”

Section: Determination Of Homovanillic Acidmentioning

confidence: 99%

Plasma levels of benperidol, prolactin, and homovanillic acid after intravenous versus two different kinds of oral application of the neuroleptic in schizophrenic patients

Seiler

Wetzel²,

Hillert³

et al. 2009

Exp Clin Endocrinol Diabetes

View full text Add to dashboard Cite

show abstract

“…Other reported methods are not completely satisfactory: they use high volumes of plasma (1 mL) and have high analytical variability (coefficient of variation up to 10%) [26], or have not satisfactory sensitivity (LOD = 2.0 ng mL −1 ), probably due to the use of protein precipitation [15,28] which introduces a dilution step into the procedure.…”

Section: Introductionmentioning

confidence: 98%

“…Several analytical methods have been published for the determination of HVA in different biological matrices including urine [16,17], cerebro-spinal fluid [18], brain tissues [19], animal plasma [20,21] and other tissues [22]. Other methods regard the analysis of this compound in human plasma; the vast majority of them are based on the use of HPLC with electrochemical [15,21,[23][24][25][26][27][28][29] detection, however at least one uses HPLC with fluorescence detection [17] and another capillary gas chromatography-mass spectrometry [30].…”

Section: Introductionmentioning

confidence: 98%

Determination of homovanillic acid (HVA) in human plasma by HPLC with coulometric detection and a new SPE procedure

Saracino

Mandrioli

Mercolini

et al. 2006

Journal of Pharmaceutical and Biomedical Analysis

View full text Add to dashboard Cite

Catatonia: short-term response to lorazepam and dopaminergic metabolism

et al. 1995

View full text Add to dashboard Cite

Therapeutic response to lorazepam and dopaminergic metabolism were investigated in 18 neuroleptically naive acute catatonic patients. They were diagnosed as catatonic according to criteria by Lohr and Rosebush and treated exclusively with lorazepam (2-4 mg) during the first 24 h. Dopaminergic metabolism (plasma HVA, plasma MHPG), anxiety (HAM-A) and parkinsonic/dyskinetic movements (SEPS, AIMS) were measured under standard conditions before initial treatment with lorazepam (day 0) and 24 h after initial treatment (day 1). On day 0 responders to lorazepam treatment (complete remission of catatonic syndrome after 24 h according to Rosebush and Lohr) showed significantly higher (P = 0.004) plasma HVA (130.4 +/- 51.2 pmol/ml; means +/- SD) than non-responders (no remission of catatonic syndrome after 24 h; 73.2 +/- 40.5 pmol/ml; means +/- SD). On day 1 plasma HVA did not differ any more significantly between both groups Clinically, responders showed significantly higher HAM-A (P = 0.025) and AIMS (P = 0.022) scores as well as significantly lower SEPS (P = 0.049) scores than non-responders on day 0. Hence catatonic short-term responders and nonresponders to lorazepam can be distinguished with regard to plasma HVA, anxiety and dyskinetic/parkinsonic movements.

show abstract

Determination of Homovanillic Acid in Human Plasma Using HPLC with Electrochemical Detection and Automated Solid Phase Extraction

Cited by 7 publications

References 10 publications

Plasma levels of benperidol, prolactin, and homovanillic acid after intravenous versus two different kinds of oral application of the neuroleptic in schizophrenic patients

Plasma levels of benperidol, prolactin, and homovanillic acid after intravenous versus two different kinds of oral application of the neuroleptic in schizophrenic patients

Determination of homovanillic acid (HVA) in human plasma by HPLC with coulometric detection and a new SPE procedure

Catatonia: short-term response to lorazepam and dopaminergic metabolism

Contact Info

Product

Resources

About