Determination of homocysteine thiolactone, reduced homocysteine, homocystine, homocysteine–cysteine mixed disulfide, cysteine and cystine in a reaction mixture by overimposed pressure/voltage capillary electrophoresis

Zinellu, Angelo; Sotgia, Salvatore; Scanu, Bastianina; Pisanu, Elisabetta; Sanna, Manuela; Sati, Satish; Deiana, Luca; Sengupta, Shantanu; Carru, Ciriaco

doi:10.1016/j.talanta.2010.06.054

Cited by 34 publications

(24 citation statements)

References 26 publications

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“…One important set of homocysteine reactions in the body is the oxidation of thiol groups between homocysteine molecules and cysteine residues in other proteins to form disulfide bonds (also called disulfide bridges) [55,56]. Another set of bodily reactions of homocysteine is N -homocysteinylation.…”

Section: Mechanistic Studiesmentioning

confidence: 99%

Vitamin B12 and Semen Quality

Banihani

2017

Biomolecules

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Various studies have revealed the effects of vitamin B12, also named cobalamin, on semen quality and sperm physiology; however, these studies collectively are still unsummarized. Here, we systematically discuss and summarize the currently understood role of vitamin B12 on semen quality and sperm physiology. We searched the Web of Science, PubMed, and Scopus databases for only English language articles or abstracts from September 1961 to March 2017 (inclusive) using the key words “vitamin B12” and “cobalamin” versus “sperm”. Certain relevant references were included to support the empirical as well as the mechanistic discussions. In conclusion, the mainstream published work demonstrates the positive effects of vitamin B12 on semen quality: first, by increasing sperm count, and by enhancing sperm motility and reducing sperm DNA damage, though there are a few in vivo system studies that have deliberated some adverse effects. The beneficial effects of vitamin B12 on semen quality may be due to increased functionality of reproductive organs, decreased homocysteine toxicity, reduced amounts of generated nitric oxide, decreased levels of oxidative damage to sperm, reduced amount of energy produced by spermatozoa, decreased inflammation-induced semen impairment, and control of nuclear factor-κB activation. However, additional research, mainly clinical, is still needed to confirm these positive effects.

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Section: Mechanistic Studiesmentioning

confidence: 99%

Vitamin B12 and Semen Quality

Banihani

2017

Biomolecules

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“…The importance to study redox status of various biological thiols including Hcy and its follow‐up compounds HCy thiolactone (HTL) was emphasized in a very good paper by Zinellu et al. , who used a pressure‐imposed CE separation of Hcy, Cys, and their intermediates. The separation is shown in Fig.…”

Section: Ce With Uv‐vis Detectionmentioning

confidence: 99%

Capillary electrophoresis in the analysis of biologically important thiols

2016

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In this review article, CE methods for analysis of biologically important thiols are overviewed. The article covers the period from the previously published comprehensive review in 2004 until mid-2016, with emphasis on various detection modes, novel approaches for sample preconcentration, and applications in clinical practice. The most commonly used detection methods, such as conductometry or absorbance detection, although universally applicable and available in most commercial instruments have low sensitivity and have only limited use in thiol analysis. Amperometric and MS detection are more sensitive and have their steady place in thiol analysis, although the mainstay remains CE with LIF detection, reaching nanomolar concentration sensitivities for most of the thiols. Novel probes for CE-LIF have been developed and tested. The preconcentration approaches using modified gold nanoparticles reaching excellent sensitivities in the picomolar range and various sample stacking methods are also reviewed. Finally, significant clinical applications of the developed methods are discussed with critical insights into the future of CE analysis of thiols.

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“…Up till now, various methods for the determination of thiol compounds have been used, especially UV-Vis spectrophotometry [8,9], spectrofluorimetry [10,11], electrochemical method [12][13][14], MS [15,16], LC [17,18], and CE [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Rapid and sensitive determination of free thiols by capillary zone electrophoresis with near‐infrared laser‐induced fluorescence detection using a new BODIPY‐based probe as labeling reagent

Zhang

Guo

et al. 2014

Electrophoresis

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A CZE with near-infrared (NIR) LIF detection method has been developed for the analysis of six low molecular weight thiols including glutathione, homocysteine, cysteine, γ-glutamylcysteine, cysteinylglycine, and N-acetylcysteine. For this purpose, a new NIR fluorescent probe, 1,7-dimethyl-3,5-distyryl-8-phenyl-(4'-iodoacetamido)difluoroboradiaza-s-indacene was utilized as the labeling reagent, whose excitation wavelength matches the commercially available NIR laser line of 635 nm. The optimum procedure included a derivatization step of the free thiols at 45°C for 25 min and CZE analysis conducted within 14 min in the running buffer containing 16 mmol/L pH 7.0 sodium citrate and 60% v/v ACN. The LODs (S/N = 3) ranged from 0.11 nmol/L for N-acetylcysteine to 0.31 nmol/L for γ-glutamylcysteine, which are better than or comparable to those reported with other derivatization-based CE-LIF methods. As the first trial of NIR CE-LIF method for thiol determination, the practical application of the proposed method has been validated by detecting thiols in cucumber and tomato samples with recoveries of 96.5-104.3%.

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Determination of homocysteine thiolactone, reduced homocysteine, homocystine, homocysteine–cysteine mixed disulfide, cysteine and cystine in a reaction mixture by overimposed pressure/voltage capillary electrophoresis

Cited by 34 publications

References 26 publications

Vitamin B12 and Semen Quality

Vitamin B12 and Semen Quality

Capillary electrophoresis in the analysis of biologically important thiols

Rapid and sensitive determination of free thiols by capillary zone electrophoresis with near‐infrared laser‐induced fluorescence detection using a new BODIPY‐based probe as labeling reagent

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