Determination of “Hoffmann Analytes” in Cigarette Mainstream Smoke. The Coresta 2006 Joint Experiment

Abstract

Regulatory authorities are currently discussing the measurement of and imposition of ceilings on certain smoke analytes, the so called "Hoffmann analytes". However, as a prerequisite, the measurement methods and the tolerances around the measurements first need to be established. In 1999, the Cooperation Centre for Scientific Research Relative to Tobacco (CORESTA) set up a Task Force "Special Analytes" to deal with analytical methodology for measuring "Hoffmann analytes" under International Standard (ISO) smoking and to work towards the standardisation of methods. This paper describes the output and conclusions from a 2005-2006 joint experiment made within the Task Force representing laboratories currently able to analyse these compounds. Data were obtained on most "Hoffmann analytes" from reference cigarettes (2R4F and 1R5F), collecting data according to the existing methods used by the nineteen participating laboratories, in order to describe the within and among laboratory variability and to see which methods could most benefit from more rigorous standardisation work. In some cases, the applied statistical analysis found that methods could not well differentiate the 1R5F and 2R4F cigarettes of differing 'tar' yield. This was explained, in part, by the broad range of methods used by the participating laboratories but also indicated that there were significant inadequacies in the choice of some methods or weaknesses in their application. Results indicate that "Hoffmann Analyte" data are generally more variable both within and among laboratories than nicotine free dry particulate matter (NFDPM); nicotine and carbon monoxide due to their lower smoke yields. Accordingly, tolerances around methods adopted for regulatory purposes will need to be proportionately higher. Methods for benzo[a]pyrene (B[a]P) and tobacco-specific nitrosamines (TSNAs), already taken to CORESTA recommended methods or ISO standardised methods through the efforts of this Task Force, give some of the most reproducible results, showing the value of this process. However, these data strongly suggest that even these analytes have much higher among-laboratory variability than for NFDPM, nicotine and CO and, based on the only two available one point in time studies, may need tolerances in the range of 35-45% for B[a]P and 26-55% for TSNAs, if they are to be measured for regulatory purposes. The collected data is useful to participating laboratories for internal method validation and laboratory accreditation, and data comparisons with others allow laboratories to identify strengths and weaknesses in their current methods. However, much work still needs to be carried out to take most of the methods towards standardisation. Although some fundamental differences or areas of concern around the methodology are discussed herein, they are not comprehensive and there may be others that need to be addressed before methods can be considered ready to take to a Recommended Method and/or to an ISO Standard. These methodological issues are being addressed in furth...

Variation in tobacco and mainstream smoke toxicant yields from selected commercial cigarette products

“…Repeated measures across more than one laboratory will increase the variability substantially (Gregg et al, 2004. Intorp et al, 2009.…”

“…The maximum 2CV values for TNCO determined in this single-centre study under ISO were 14.4%. If closer to 100% coverage of data were required, then an interval of 3SD (99.7%, empirically) would be more appropriate; and, if measurements were carried out at more than one laboratory, then inter-laboratory variation would also need to be taken into account, again increasing the interval substantially (Intorp et al, 2009;Teillet et al, 2013).…”

“…Many studies have estimated the variability of various smoke analyte measurements within a single laboratory (Rickert and Wright, 2002) and across several laboratories (Hyodo et al, 2006;Intorp et al, 2009;Teillet et al, 2013;Purkis and Intorp, 2014). These studies, using reference cigarettes, have concluded that smoke toxicant measurements are generally more variable as compared with measurements of tar, nicotine and carbon monoxide (TNCO), both within and among laboratories, and that measurements are more variable among laboratories than within a single laboratory.…”

Determination of Selected Volatiles in Cigarette Mainstream Smoke. The CORESTA 2008 Joint Experiment

“…As in other recent CORESTA Task Force work, each laboratory was requested to follow its own in-house methods analysing as many of the five analytes as was their normal practice. The 2R4F Kentucky reference cigarette used in the previous study (9) has been superseded by the 3R4F although the former and latter give similar "nicotine-free dry particulate matter" (NFDPM) yields. The 3R4F and 1R5F reference cigarettes were used for testing, each sourced from a single batch isolated by the University of Kentucky.…”

“…Here, it is noted that on the NFDPM scale these two samples are distinctly different, with average yields of approximately 1.7 and 9.5 mg/cig, respectively. This approach has been described in the last two reports from this Task Force (9,11). The statistical equation is provided in Table 11 alongside the results of the statistical analysis.…”