Determination of Histamine by Chemical Means

McIntire, Floyd C.

doi:10.1007/978-3-662-12016-3_3

Cited by 6 publications

(3 citation statements)

References 40 publications

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“…It is even more difficult to detect con comitantly occurring histamine metabolites since these compounds are structurally very closely related to histamine. Current methods for the detection of histamine and several of its derivatives include diazotized sulfanilic acid (19), p-nitroaniline (19,25), dinitrofluorobenzene (17,19,23), o-phthaldialdehyde (19,31) and iodine (5,28). In none of the above mentioned papers have detection limits been described.…”

Section: Discussionmentioning

confidence: 99%

Determination of Histamine and Its Metabolic Products in the Pig Gastric Mucosa

Schippert¹,

Kovar²,

Sewing³

1979

Pharmacology

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This paper describes analytical techniques for quantitative and qualitative determination of histamine and its metabolites (N^τ-methylhistamine, N^α-methylhistamine and N^α, N^α-dimethylhistamine) in the pig gastric mucosa. These metabolites and N^α-acetylhistamine, imidazolyl-4-acetic acid and N^τ-methylimidazolylacetic acid were synthesized as reference compounds and analyzed by using the dansylation technique. TLC of dansylated mucosal extracts in various solvents {in situ fluorescence measurements) in combination with TLC/IR and TLC/MS transfer technique demonstrated the presence of histamine. The only metabolite was N^τ-methyl-histamine.

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Section: Discussionmentioning

confidence: 99%

Determination of Histamine and Its Metabolic Products in the Pig Gastric Mucosa

Schippert¹,

Kovar²,

Sewing³

1979

Pharmacology

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“…used for measuring biogenic amines (see Euler 1961;Udenfriend 1962Udenfriend & 1969Sandler 1963Sandler & 1968Garattini & Valzelli 1965;Haggendal 1966;Hanson 1966;McIntire 1966;Callingham 1967;Sharman 1971;Weil-Malherbe 1971;Anton & Sayre 1972;Atack 1977), although for certain applications other important techniques are also available including: bioassay (see Gaddum 1959 ;Code & McIntire 1956;Vugman & Rocha e Silva 1966;Vane 1966;Sharman 1971); enzymatic isotope dilution derivative techniques (see Engelman 1972;Saavedra et al 1973;Beaven 1975) which have proved particularly important for Hm (see Snyder & Taylor 1972); and mass spectrometry preceded by gas chromatographical purification Cattabeni et al 1972;see Costa et al 1975). The fluorimetric assays are relatively easy and inexpensive to perform, yet are frequently of adequate sensitivity and specificity when preceded by complementary procedures for purifying and concentrating the extracted amines (see reviews of fluorimetric assays above).…”

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confidence: 99%

A Procedure for the Isolation of Noradrenaline (together with Adrenaline), Dopamine, 5‐Hydroxytryptamine and Histamine from the Same Tissue Sample Using a Single Column of Strongly Acidic Cation Exchange Resin

Atack

Magnusson

1978

Acta Pharmacologica et Toxicologica

128

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Using a single column of a strongly acidic cation exchange resin (Dowex 50W, X-4, 200-400 mesh, resin bed 4.0 mm diameter x 75 mm long in sodium form), noradrenaline (NA) (together with adrenaline (A)), dopamine (DA), 5-hydroxytryptamine (5-HT), and histamine (Hm), are recovered in separate eluates after a single extraction of the same tissue sample into perchloric acid. Recoveries of the amines from homogenates and filtrates were approximately the same: for NA (together with A), about 90% in a 7-ml eluate; for DA, about 95% in 3.5 ml; for 5-HT, about 75% in 7 ml; and for Hm, about 85% in 4.5 ml. The establishment and routine use of the procedure are presented in detail. Several metabolites of the amines, including 5-hydroxyindole-3-acetic acid (5-HIAA), the precursor amino acids, and 3-methoxytyramine (3-MT), can be recovered from the same Dowex 50 column by incorporating the procedures developed by Carlsson, Kehr & Lindqvist in this laboratory. All the amines and many of their metabolites can be purified successively into their eluates in a normal working day. We now have increased opportunities to study aspects of the metabolism of the biogenic amines. Thus, for the catecholamines can be monitored tyrosine + DOPA + DA + NA + A and also DA + 3-MT; and for 5-HT, the metabolic pathway tryptophan + 5-hydroxytryptophan + 5-HT + 5-HIAA. The column purification contributes considerable specificity to the overall procedure. Most compounds which are acidic; e.g. 5-HIAA, neutral or amphoteric, e.g. all the precursor amino acids, are eluted before the amines. Considerable resolution of basic compounds into separate fractions is also achieved : notably Hm is eluted before spermidine, and DA is separated from NA plus A. Thus, many compounds are prevented from interfering in fluorimetric assays which have been especially adapted for measuring these amines in the Dowex 50 eluates. Salt equivalent to at least 10 g of tissue placed on the column does not affect the recoveries of the amines. For quantitative determinations of endogenous amines, approximately 10 ng of each amine on the Dowex 50 column are sufficient, and smaller amounts can be detected. The procedure was developed particularly for measuring amines in nervous tissue, but it has been applied successfully to several peripheral tissues and body fluids.

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“…The resins selected for the experiments were all of the weak cationic type: methacrylic, carboxylic, and phenolic forms. This type of resin is, in fact, most selective for histamine (18). For purposes of comparison, a strong sulfonic cation and a synthetic zeolite were incorporated into the study.…”

mentioning

confidence: 99%

Dehistaminization of Organ Extracts by Ion-Exchange Chromatography

Tartarini¹

1972

Journal of Pharmaceutical Sciences

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Determination of Histamine by Chemical Means

Cited by 6 publications

References 40 publications

Determination of Histamine and Its Metabolic Products in the Pig Gastric Mucosa

Determination of Histamine and Its Metabolic Products in the Pig Gastric Mucosa

A Procedure for the Isolation of Noradrenaline (together with Adrenaline), Dopamine, 5‐Hydroxytryptamine and Histamine from the Same Tissue Sample Using a Single Column of Strongly Acidic Cation Exchange Resin

Dehistaminization of Organ Extracts by Ion-Exchange Chromatography

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