Determination of hesperetin and its conjugate metabolites in serum and urine

Effects of hesperidin combined with synephrine on the capture of acrolein in a mouse model, or in humans by citrus consumption

“…The levels of both HES and HESP in the urine samples tended toward 0 after 14 h of citrus consumption, similar to the findings of previous studies. 44,48,49 Notably, the amount of HESP was 10-fold higher than that of HES, suggesting that the majority of HES is converted into HESP via the cleavage of the rutin moiety in vivo, which is consistent with previous literature 40 and our mouse experimental data (Fig. 5A).…”

“…Second, the amount of HESP-ACR was far greater than that of HES-ACR-1, both in the urine and fecal samples of mice. This can be attributed to the fact that the majority of HES is metabolized into HESP in vivo , 44 and HESP further captures ACR to form the adduct, as previously reported in vitro by Wang. 26…”

“…Second, the amount of HESP-ACR was far greater than that of HES-ACR-1, both in the urine and fecal samples of mice. This can be attributed to the fact that the majority of HES is metabolized into HESP in vivo, 44 and HESP further captures ACR to form the adduct, as previously reported in vitro by Wang. 26 Third, considering that HES and SYN coexist in plenty of fruits and their products, such as, oranges, lemons, mandarins, and Flos Citri Aurantia, [17][18][19][20] we designed a combination of HES and SYN with which mice were given gavage during the mouse experiment.…”

Ultra-Performance Liquid Chromatography-Ion Mobility Separation-Quadruple Time-of-Flight MS (UHPLC-IMS-QTOF MS) Metabolomics for Short-Term Biomarker Discovery of Orange Intake: A Randomized, Controlled Crossover Study

“…These compounds have been previously studied due to their antioxidants and anti-inflammatory properties [ 48 , 49 ]. Moreover, hesperitin phase I/II conjugates have already been described as major metabolites of other citrus containing the flavonoids hesperidin and naringenin [ 50 , 51 ].…”

Φαρμακοτεχνική - Φαρμακοκινητική Μελέτη Δραστικών Φλαβονοειδών

“…Η διεξαγωγή φαρµακοκινητικών µελετών στους ανθρώπους, µετά από του στόµατος χορήγηση των φλαβονοειδών γλυκοζιτών εσπεριδίνης και ναριγγίνης, απαιτεί µια εκλεκτική, ευαίσθητη, ακριβή, επαναλήψιµη και γενικά αξιόπιστη αναλυτική µέθοδο για τον ταυτόχρονο προσδιορισµό των αγλύκων τους, εσπερετίνης και ναριγγενίνης, στα δείγµατα πλάσµατος ή ούρων. Μέχρι σήµερα έχουν δηµοσιευθεί διάφορες HPLC µέθοδοι για τον ποσοτικό προσδιορισµό της εσπερετίνης [Tsai and Chen, 2000;Yang et al, 2002] ή της ναριγγενίνης [Fuhr and Kummert, 1995;Ishii et al, 1996;Ishii et al, 1997;Peng et al, 1998…”