Determination of hemicellulose, cellulose, holocellulose and lignin content using FTIR in Calycophyllum spruceanum (Benth.) K. Schum. and Guazuma crinita Lam.

Abstract

Capirona (Calycophyllum spruceanum (Benth.) K. Schum.) and Bolaina (Guazuma crinita Lam.) are fast-growing Amazonian trees with increasing demand in timber industry. Therefore, it is necessary to determine the content of cellulose, hemicellulose, holocellulose and lignin in juvenile trees to accelerate forest breeding programs. The aim of this study was to identify chemical differences between apical and basal stem of Capirona and Bolaina to develop models for estimating the chemical composition using Fourier transform infrared (FTIR) spectra. FTIR-ATR spectra were obtained from 150 samples for each species that were 1.8 year-old. The results showed significant differences between the apical and basal stem for each species in terms of cellulose, hemicellulose, holocellulose and lignin content. This variability was useful to build partial least squares (PLS) models from the FTIR spectra and they were evaluated by root mean squared error of predictions (RMSEP) and ratio of performance to deviation (RPD). Lignin content was efficiently predicted in Capirona (RMSEP = 0.48, RPD > 2) and Bolaina (RMSEP = 0.81, RPD > 2). In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition. In Bolaina, model for cellulose attained an excellent predictive power (RMSEP = 1.82, RPD = 6.14) while models for hemicellulose and holocellulose attained a good predictive power (RPD > 2.0). This study showed that FTIR-ATR together with PLS is a reliable method to determine the wood chemical composition in juvenile trees of Capirona and Bolaina.

Characterisation of Hemp Fibres Reinforced Composites Using Thermoplastic Polymers as Matrices

“…The peak located at 1315 cm −1 in the FTIR spectrum of the hemp, attributed to the rocking vibrations of the C–H bonds from the CH 2 groups of cellulose, was not found in the FTIR spectra of the rigid composites that contain hemp [ 64 ]. The peak recorded at 1026 cm-1 in the FTIR spectrum of hemp, attributed to the stretching vibrations of the C–O bonds (C–O–C glycosidic linkages from cellulose and hemi-cellulose, C–OH phenolic groups from lignin and C–O–C ether linkages from lignin, respectively), decreased greatly in intensity as the amount of hemp in the composites decreased [ 65 ]. The decrease in the peak intensity once the PP/H ratio had increased may be due to the steric hindrance that occurs as the amount of PP in the composite increases.…”

“…The peak detected around 872 cm −1 is attributed to the stretching vibrations of the C–O bond from the polyester. The peak recorded around 840 cm −1 is assigned to the out-of-plane stretching vibrations of the C–H bonds from the aromatic rings present in the lignin [ 65 ]. The presence of the peaks located at 721, 723 and 725 cm −1 is related to the out-of-plane stretching vibrations of the C–H bonds from the aromatic ring [ 66 ].…”

Pb(II) Ion Removal Potential in Chemically Modified Ziziphus joazeiro Barks

“…Analyzing the FT-IR spectrum, the peak of 3360.04 cm −1 was shifted to 3401.63 cm −1 , with a significant increase in intensity and width after the alkaline treatment. These peaks are commonly associated with intra- and intermolecular -OH stretching vibration from cellulose, lignin and hemicellulose [ 81 ], in addition to saponins [ 82 ]; such behavior was also observed in the treatment performed by [ 12 , 83 ]. The 2933.33 cm −1 peak referring to the stretching of the C-H bonds of the hydrocarbons became more discreet, while those in the 1200 to 1500 cm −1 range showed a number of peaks and a reduction in width.…”

“…The peak of 1036.65 cm −1 refers to the C-O stretches of holocellulose and lignin [ 81 ], which may come from ether and phenol [ 12 ], or the carboxylic and alcohol groups [ 86 ], and can also be attributed to the linkage absorption C-O-C [ 87 ] to oligosaccharide present in the structure of saponin [ 27 , 88 ], assuming that this substance has not been completely removed by contact with 0.5 N NaOH, as the alkaline treatment generally induces extensive modifications in the polysaccharides [ 89 , 90 ]. After contact with Pb(II) ions, the enlargement and increase in intensity of this region can be noticed.…”

Comparative study of the Rheum tanguticum’s chemical contents based on spatial distribution characteristics

“…Fourier-transform infrared spectroscopy (FTIR) and high-performance liquid chromatography (HPLC) are two such techniques. FTIR has been widely used to determine the provenance of food and natural pharmaceuticals as well as to forecast the quantity of index components [ 36 , 37 ]. Many agencies, including the World Health Organization, Food and Drug Administration, and State Food and Drug Administration, have acknowledged HPLC fingerprint [ 38 ].…”