Determination of Helicobacter pylori CagA EPIYA types in Iranian isolates with different gastroduodenal disorders

Vaziri, Farzam; Peerayeh, Shahin Najar; Alebouyeh, Masoud; Molaei, Mahsa; Maghsoudi, Nader; Zali, Mohammad Reza

doi:10.1016/j.meegid.2013.03.048

Cited by 28 publications

(35 citation statements)

References 44 publications

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“…Several studies have showed a strong indication that the association of the virulence factors may increase the risk of the developing gastric malignant tumors Therefore, the studies of these genes in each region are important (González et al, 2011;Ramis et al, 2010;Vaziri et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Detection of Helicobacter pylori CagA EPIYA in gastric biopsy specimens and its relation to gastric diseases

Vianna

Ramis

Halicki

et al. 2015

Diagnostic Microbiology and Infectious Disease

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Section: Discussionmentioning

confidence: 99%

Detection of Helicobacter pylori CagA EPIYA in gastric biopsy specimens and its relation to gastric diseases

Vianna

Ramis

Halicki

et al. 2015

Diagnostic Microbiology and Infectious Disease

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“…Similar to our study, Kalaf et al (2013) found a significant association between multiple EPIYA-C repeats and GC (Pj0.01). Vaziri et al (2013) studied EPIYA-ABCC and EPIYA-A-B/C and showed associations with GC and duodenitis, respectively. Silva et al (2014) stated that the risk of GC was approximately fourfold higher among patients infected with strains carrying two or three EPIYA-C.…”

Section: Epiya-c and -D Have A Role In The Interaction Betweenmentioning

confidence: 99%

Patterns of EPIYA motifs among cagA-positive Helicobacter pylori strains: a case–control study in a Turkish population with Eurasian geographical features

Kocazeybek

Caliskan

Cetin

et al. 2015

Journal of Medical Microbiology

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Geographical variation in the frequency of various gastroduodenal pathologies was shown to be related to the geographical diversity of H. pylori CagA Glu-Pro-Ile-Tyr-Ala (EPIYA) patterns. We examined the EPIYA patterns of H. pylori and the association of EPIYA patterns with gastric cancer (GC) for the first time, to the best of our knowledge, in Turkey. The patient group (PG) contained 60 patients [38 GC and 22 duodenal ulcer (DU) patients]. The control group (CG) was 110 individuals [94 gastritis patients and 16 persons with a normal gastrointestinal system (NGIS)]. Specific primers were used for the detection of cagA including empty-site-positive and EPIYA-A, -B, -C, -D PCR. Bands of EPIYA-A, -B, -C were confirmed by DNA sequencing. One hundred and forty-two (83.5 %) strains [60 in the PG (38 GC, 22 DU), 82 in the CG (72 gastritis, 10 NGIS)] were positive for the cagA gene. EPIYA-C with multiple repeats was detected in 34 (23.9 %) strains, and 22 (64.7 %) were from GC patients. EPIYA-C with one repeat was detected in 89 (62.7 %) strains, and 54 (60.7 %) were from gastritis patients. EPIYT was detected in 10 strains, and EPIYA-D was not detected. The number of EPIYA-C with multiple repeats was significantly higher for the PG than for the CG (P,0.0001). In GC patients, the number of EPIYA-C with multiple repeats was significantly higher than one repeat (P,0.0001). In conclusion, our study showed that multiple EPIYA-C repeats increases the GC risk by 30.6-fold and the DU risk by 8.9-fold versus the CG. This indicates that Western-type H. pylori strains in Turkey have similar EPIYA motifs to those of neighbouring countries and Western populations. INTRODUCTIONHelicobacter pylori is the principal cause of gastritis and gastric or duodenal ulcer (DU) diseases and is involved in the development of gastric cancer (GC) and mucosaassociated lymphoid tissue lymphoma (Atherton, 2006).H. pylori is a class 1 carcinogen identified by the International Agency for Research on Cancer for chronic and persistent infections. It is resistant to antibacterial agents in spite of innate and adaptive immunity (International Agency for Research on Cancer, 1994). Depending on virulence factors such as cytotoxin-associated gene A (CagA), blood group antigen-binding adhesion (BabA2) and vacuolating cytotoxin gene A (VacA), different genotypes cause different pathological and clinical outcomes and geographical regional differences (Atherton et al., 1995;Basso et al., 2008). Recently, the Glu-Pro-Ile-Tyr-Ala (EPIYA)Abbreviations: cagPAI, cag pathogenicity island; CG, control group; CI, confidence interval; DU, duodenal ulcer; EPIYA, Glu-Pro-Ile-Tyr-Ala; CagA, cytotoxin-associated gene A; GC, gastric cancer; NGIS, normal gastrointestinal system; PG, patient group; OR, odds ratio. pattern of CagA was suggested to be involved in the pathogenesis of GC, as this pattern manifests important variations depending on the region (Argent et al., 2004(Argent et al., , 2005.The CagA protein is injected into host epithelial cells with its peptidoglycan b...

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“…Although similarities or differences between H. pylori strains from each patient could be easily predicted by comparing their RAPD patterns, this method does not provide any information about their virulence properties and evolved genetic events. Evolution of H. pylori virulence genes in the human stomach was reported by some studies (1,9,11,12,21,25 …”

Section: Introductionmentioning

confidence: 94%

“…In addition to antibiotic resistance features of the infecting strains, development of life-threatening complications is also determined by their genetic contents and virulence properties (10,11). Different manifestations of H. pylori infections among different patients could be explained by genetic diversity of the responsible strains (4).…”

Section: Introductionmentioning

confidence: 99%

Genotype Diversity and Quasispecies Development of <i>Helicobacter pylori</i> in a Single Host

et al. 2015

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SUMMARY:Infection with different strains of Helocobacter pylori and emergence of new genomic variants during their long-term gastric colonization are assumed to be the main reasons for eradication failure. We used genotyping and arbitrarily primed PCR fingerprinting (RAPD) to detect relatedness and genetic variations among H. pylori single isolates from each patient in Iran. Multiplex-PCR amplification of gene alleles encoding the virulence factors vacA (m/s), cagA, and iceA (A1/A2) and comparison of RAPD patterns of different singles colonies were performed for each individual patient's isolate. Results showed a high frequency of diversity among the H. pylori strains. Nearly 23z of infected patients showed a single type infection. The infection types related, unrelated, and related/unrelated were found among 25.6z, 12.8z, and 38.5z of patients, respectively. Both mixed type infections (77z) and quasispecies development (15.4z) were detected in these patients. Genotype conversion among vacA (41.6z), cagA (41.6z), and iceA (50z) alleles was observed for the strains with identical or related RAPD patterns. Coevolution of different alleles was also detected in a patient infected with strains presenting the same RAPD patterns. Collectively, results of this study revealed the occurrence of quasispecies development, mixed type infections, and changes in virulence properties of H. pylori strains among the studied patients.

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Determination of Helicobacter pylori CagA EPIYA types in Iranian isolates with different gastroduodenal disorders

Cited by 28 publications

References 44 publications

Detection of Helicobacter pylori CagA EPIYA in gastric biopsy specimens and its relation to gastric diseases

Detection of Helicobacter pylori CagA EPIYA in gastric biopsy specimens and its relation to gastric diseases

Patterns of EPIYA motifs among cagA-positive Helicobacter pylori strains: a case–control study in a Turkish population with Eurasian geographical features

Genotype Diversity and Quasispecies Development of <i>Helicobacter pylori</i> in a Single Host

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