Determination of heat-induced effects on the particle size distribution of casein micelles by dynamic light scattering and nanoparticle tracking analysis

Le, T. Tran; Saveyn, Pieter; Hoa, Hoang Dinh; Meeren, Paul Van der

doi:10.1016/j.idairyj.2008.06.006

Cited by 60 publications

(17 citation statements)

References 16 publications

(17 reference statements)

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“…NTA is based on a microscopic view of the particles illuminated by a laser diode. Different intensities of the scattered light are indicative of different particle sizes as larger particles scatter higher intensity light, and hence, larger luminous dots are observed [40,41]. On the other hand, smaller particles move faster and over longer distances with respect to larger particles [40].…”

Section: Resultsmentioning

confidence: 99%

Hydrogels containing redispersible spray-dried melatonin-loaded nanocapsules: a formulation for transdermal-controlled delivery

et al. 2012

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The aim of the present study was to develop a transdermal system for controlled delivery of melatonin combining three strategies: nanoencapsulation of melatonin, drying of melatonin-loaded nanocapsules, and incorporation of nanocapsules in a hydrophilic gel. Nanocapsules were prepared by interfacial deposition of the polymer and were spray-dried using water-soluble excipients. In vitro drug release profiles were evaluated by the dialysis bag method, and skin permeation studies were carried out using Franz cells with porcine skin as the membrane. The use of 10% (w/v) water-soluble excipients (lactose or maltodextrin) as spray-drying adjuvants furnished redispersible powders (redispersibility index approximately 1.0) suitable for incorporation into hydrogels. All formulations showed a better controlled in vitro release of melatonin compared with the melatonin solution. The best controlled release results were achieved with hydrogels prepared with dried nanocapsules (hydrogels > redispersed dried nanocapsules > nanocapsule suspension > melatonin solution). The skin permeation studies demonstrated a significant modulation of the transdermal melatonin permeation for hydrogels prepared with redispersible nanocapsules. In this way, the additive effect of the different approaches used in this study (nanoencapsulation, spray-drying, and preparation of semisolid dosage forms) allows not only the control of melatonin release, but also transdermal permeation.

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Section: Resultsmentioning

confidence: 99%

Hydrogels containing redispersible spray-dried melatonin-loaded nanocapsules: a formulation for transdermal-controlled delivery

et al. 2012

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“…The main used techniques for the analysis of PS and particle size distribution (PSD) are DLS, nanoparticle tracking analysis (NTA), scanning electron microscopy (SEM), size exclusion chromatography (SEC), cell electrophoresis, analytical ultracentrifugation (AUC), etc. Various analytical techniques may give different results [31][32][33][34]. DLS is the most user-friendly and it gives relatively accurate and consistent results of protein samples which can be obtained in a short period of time [35].…”

Section: Measurement Of Casein Micelle Size By Dynamic Light Scatterimentioning

confidence: 99%

Measurement of Casein Micelle Size in Raw Dairy Cattle Milk by Dynamic Light Scattering

Hristov¹,

Mitkov²,

Sirakova³

et al. 2016

Milk Proteins - From Structure to Biological Properties and Health Aspects

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The particle size of milk influences its microstructure and defines the qualities of dairy products, such as colloidal stability and texture. Moreover, differences in casein micelle size may affect milk processing, especially cheese making. Hence, the size of casein micelle is an important characteristic of raw milk and determines the yield of dairy products. The aim of the present research is to estimate the casein micelle size in the raw milk of dairy cattle by dynamic light scattering. The obtained results may be used for genetic elaboration of the breed, as well as to increase the competitiveness of the milk industry by selection of animals with higher casein micelle size.

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“…Indeed, the hydrodynamic diameter measured in water (around 150 nm) is in agreement with values determined by others. 49 Upon addition of NaCl, no significant changes in micellar size were reported. Indeed, it was demonstrated in concentrated and unconcentrated milk that the average particle size of casein micellar remain unchanged during salt addition.…”

Section: Casein Micelle Size Morphology and Repartition Associated Wmentioning

confidence: 99%

Multiscale Characterization of Casein Micelles Under NaCl Range Conditions

et al. 2011

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Micellar casein (MC) dispersions were studied at a constant protein concentration of 5 wt % in high NaCl environment. The micellar edifices were characterized as to their morphology, size, and content of proteins in the supernatant after ultracentrifugation. Additionally, changes in secondary structures of the protein upon salt increase were followed by Fourier Transform Infrared Spectroscopy (FTIR). For the first time, the estimations of secondary structural elements (irregular, ß-sheet, α-helix and turn) from Amide III assignments were correlated with results from Amide I. Casein micelles dispersions in water were characterized by Transmission Electron Microscopy (TEM) by a spherical shape and a size between 100 and 200 nm. A salt increase resulted to a destabilization of the micelle and the formation of mini-micelles more or less aggregated. The size of the new edifice was almost similar to the native micelle. These TEM observations were confirmed by a constant casein micelle hydrodynamic diameter determined by Dynamic Light Scattering (DLS) and ranging between 150 and 180 nm. Upon salt increase, FTIR revealed an increase in irregular structures and a concurrent decrease in ß-sheet structures. Secondary structural elements percentages were almost similar from Amide I and Amide III. The use of these multiscale techniques led to a better understanding of the micellar edifice under high salt environment. Around 3% NaCl addition, a good correlation was observed between destabilization of the micellar edifice, modifications of the caseins secondary structure and repartition of caseins between supernatant and pellet after ultracentrifugation.

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Determination of heat-induced effects on the particle size distribution of casein micelles by dynamic light scattering and nanoparticle tracking analysis

Cited by 60 publications

References 16 publications

Hydrogels containing redispersible spray-dried melatonin-loaded nanocapsules: a formulation for transdermal-controlled delivery

Hydrogels containing redispersible spray-dried melatonin-loaded nanocapsules: a formulation for transdermal-controlled delivery

Measurement of Casein Micelle Size in Raw Dairy Cattle Milk by Dynamic Light Scattering

Multiscale Characterization of Casein Micelles Under NaCl Range Conditions

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