Determination of growth and lysis kinetics in plant cell suspension cultures from the measurement of esterase release

Steward, Nicolas; Martin, Richard J.; Engasser, Jean‐Marc; Goergen, Jean‐Louis

doi:10.1002/(sici)1097-0290(1999)66:2<114::aid-bit4>3.0.co;2-w

Cited by 20 publications

(7 citation statements)

References 37 publications

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“…Two showed transient expression in mid-exponential phase, an ethylene response factor (ERF) B-1 (PO_33_F04) and an ERF subfamily B-3 (PO_13_F11), while the other three were upregulated during exponential phase: an ERF subfamily B-4 (PO_05_C10), an ethylene receptor (PO_13_F07) and an ethylene-insensitive 3 (EIN3, PO_23_B12). The upregulation of ethylene response-related cDNAs observed after mid-exponential phase is consistent with our understanding that stress factors such as shear force, nutrient starvation, production of reactive oxygen species and accumulation of ethylene cause cell ageing and death at the end of stationary phase if the cells are not subcultured (Peters et al 2000, Steward et al 1999, Zhao et al 2005.…”

Section: Regulation Of Cell Cycle-related Gene Expressionsupporting

confidence: 85%

Growth‐phase‐dependent gene expression profiling of poplar (Populus alba × Populus tremula var. glandulosa) suspension cells

Lee

Bae

Park

et al. 2007

Physiologia Plantarum

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Complex sequences of morphological and biochemical changes occur during the developmental course of a batch plant cell culture. However, little information is available about the changes in gene expression that could explain these changes, because of the difficulties involved in isolating specific cellular events or developmental phases in the overlapping phases of cell growth. In an attempt to obtain such information we have examined the global growth phase-dependent gene expression of poplar cells in suspension cultures by cDNA microarray analysis. Our results reveal that significant changes occur in the expression of genes with functions related to protein synthesis, cell cycling, hormonal responses and cell wall biosynthesis, as cultures progress from initiation to senescence, that are highly correlated with observed developmental and physiological changes in the cells. Genes encoding protein kinases, calmodulin and proteins involved in both ascorbate metabolism and water-limited stress responses also showed strong stage-specific expression patterns. Our report provides fundamental information on molecular mechanisms that control cellular changes throughout the developmental course of poplar cell cultures.

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Section: Regulation Of Cell Cycle-related Gene Expressionsupporting

confidence: 85%

Growth‐phase‐dependent gene expression profiling of poplar (Populus alba × Populus tremula var. glandulosa) suspension cells

Lee

Bae

Park

et al. 2007

Physiologia Plantarum

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“…Although esterases have long been used in plants as biomarkers for genotyping in zymogen analysis of crops and weeds (Wouters and Booy 2000) and markers for cell viability (Steward et al 1999), remarkably little is known about the functions of these enzymes in planta. Similarly, the genes encoding esterases active in pesticide metabolism have not been identified in plants.…”

Section: Discussionmentioning

confidence: 99%

Biochemical characterisation of esterases active in hydrolysing xenobiotics in wheat and competing weeds

Cummins

Burnet

Edwards

2001

Physiologia Plantarum

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Esterase activities toward model xenobiotic substrates (p‐nitrophenyl acetate, naphthyl acetate) and pesticide esters (diclofop methyl, bromoxynil octanoate, binapacryl) have been compared in crude extracts from wheat (Triticum aestivum L.) and Triticum progenitors of wheat. Esterase activities were also determined in the weeds, wild oat (Avena fatua) and two populations of black‐grass (Alopecurus myosuroides), one of which (Rothamsted) is susceptible to herbicides, while the other (Peldon) shows cross‐resistance to multiple classes of herbicides. Esterase activity toward the model substrates was highest in wheat, while the weeds were more active in hydrolysing the pesticides. Using isoelectric focussing (pH 4–8), 13 proteins with esterase activity toward α‐naphthyl acetate could be resolved in hexaploid wheat (genome AABBDD). The pattern of these activities was most similar to that of the diploid progenitor T.tauschii (DD), excepting a major acidic esterase (pI 4.6), which originated from T. urartu (AA). Resolved esterase activities in the weeds were distinct from those observed in the Tritcum species. However, unlike the case with other classes of xenobiotic‐metabolising enzymes, the complement of esterases in the Peldon and Rothamsted populations of black‐grass appeared to be identical. In all species, the more basic esterases (>pI 5.0) were sensitive to inhibition by organophosphate and carbamate insecticides, suggesting that they were B‐class esterases. In contrast, the acidic wheat esterase (pI 4.6) with the greatest activity toward α‐naphthyl acetate was insensitive to insecticides. This wheat‐specific esterase was purified 7000‐fold by a combination of hydrophobic interaction chromatography, gel filtration and anion‐exchange chromatography. The purified esterase behaved as a monomeric 45‐kDa protein showing high activity toward p‐nitrophenyl acetate and α‐naphthyl acetate, but limited activity toward the pesticides.

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“…The FDA staining indicates the esterase activity by the multiple esterase enzymes in the viable cell. In this context, the fluorescence of FDA staining does not verify the metabolism activity of the cell (Steward et al, 1999a(Steward et al, , 1999b. We should limit our conclusion to the esterase activity of the Nostoc sp.…”

Section: Revitalization and Recovery Of The Growth Of Nostoc Sp Hk-0mentioning

confidence: 99%

Growth of terrestrial cyanobacterium, Nostoc sp., on Martian Regolith Simulant and its vacuum tolerance

et al. 2008

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The growth of terrestrial cyanobacterium, Nostoc sp., on the Martian Regolith Simulant (MRS) and its vacuum tolerance were studied as one of our challenges in this century to inhabit Mars. The viability of the tested cyanobacteria was evaluated by microscopic observation after staining by fluorescein diacetate (FDA). The general terrestrial cyanobacterial lump collected from the ground showed a significantly high tolerance to a high vacuum environment (10 -5 Pa) for over one year. To scientifically elucidate its high tolerance function, Nostoc sp. HK-01 was used as another suitable scientific material for this study. After exposure to the high vacuum environment (10 -5 Pa) for two weeks, Nostoc sp. HK-01 began to grow again. Some of it was also re-incubated again with a liquid culture medium. The A'MED (Arai's Mars Ecosystem Dome) is designed to be installed on Mars for agricultural production. A'MED was useful to conduct our study. We performed the fundamental experiment using the MRS. Nostoc sp. HK-01 was found to grow for over 140 days along with having the normal function of chlorophyll synthesis on the MRS. These results show the possibility that cyanobacteria could adapt to the MRS, and grow under the low pressure environment expected on Mars.

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Determination of growth and lysis kinetics in plant cell suspension cultures from the measurement of esterase release

Cited by 20 publications

References 37 publications

Growth‐phase‐dependent gene expression profiling of poplar (Populus alba × Populus tremula var. glandulosa) suspension cells

Growth‐phase‐dependent gene expression profiling of poplar (Populus alba × Populus tremula var. glandulosa) suspension cells

Biochemical characterisation of esterases active in hydrolysing xenobiotics in wheat and competing weeds

Growth of terrestrial cyanobacterium, Nostoc sp., on Martian Regolith Simulant and its vacuum tolerance

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