Determination of Grayanotoxins in Honey by Liquid Chromatography Tandem Mass Spectrometry Using Dilute-and-Shoot Sample Preparation Approach

Kaplan, Muammer; Olgun, Elmas Öktem; Karaoğlu, Öznur

doi:10.1021/jf501560t

Cited by 20 publications

(18 citation statements)

References 15 publications

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“…The limit of detection and the limit of quantification values of the validated analysis method were 0.0033 mg/ kg and 0.01 mg/kg, respectively. The GTX-I and GTX-III amounts of the samples were found to be 32 and 8 μg/g, respectively, which were higher than the average values reported previously [13] and in parallel with the amounts of toxic substances in RH used in other studies [14][15][16] .…”

Section: Methodscontrasting

confidence: 54%

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Ormangülü Balı’nın Fare Kalp Kas Dokusu Lipitleri Üzerindeki Toksik Etkilerinin Moleküler Düzeyde İncelenmesi

Çakmak-Arslan¹,

Emir²,

Goc-Rasgele³

et al. 2019

Kafkas Univ Vet Fak Derg

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Section: Methodscontrasting

confidence: 54%

“…The amount of GTX-I and GTX-III in the RH used in this study was detected by liquid chromatography-mass/mass spectrometry (LC-MS/MS) using the method developed by Kaplan et al [13] . The limit of detection and the limit of quantification values of the validated analysis method were 0.0033 mg/ kg and 0.01 mg/kg, respectively.…”

Section: Methodsmentioning

confidence: 99%

Ormangülü Balı’nın Fare Kalp Kas Dokusu Lipitleri Üzerindeki Toksik Etkilerinin Moleküler Düzeyde İncelenmesi

Çakmak-Arslan¹,

Emir²,

Goc-Rasgele³

et al. 2019

Kafkas Univ Vet Fak Derg

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“…They were fed standard mice chow (210 kcal/100 g/day) and drank tap water. Five groups were arranged: Solutions in different concentrations, prepared with rhododendron honey (RH) from Düzce (West Black Sea, Turkey), were dissolved in water and given by gavage to the mice in 0.01 ml per gram ratio (Silici 2014).18 Test subjects of group 5 were given grayanotoxin-III (GTx) (Grayanotoxin, Lot 3-H1 293B-Enzo), approximately onetenth the rate of the concentration LD50, which is 0.908 mg/kg, so a 0.01 mg/kg dose intraperitoneally (Kaplan et al, 2014;Öztasan et al, 2005). Liver tissue samples were taken under anesthesia from half of the test subjects of each group after 24 h and the other half after 48 hours, which were identified with 10 % neutral formalin.…”

Section: Methodsmentioning

confidence: 99%

The Effect of Rhododendron Honey on Mice Liver Tissue

et al. 2016

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ILTER, G.; SÖYLER, G.; RASGELE, P. G.; KEKEÇOGLU, M & KAMBUR, M. The effect of rhododendron honey on mice liver tissue. Int. J. Morphol., 34(3): 842-847, 2016. SUMMARY: Rhododendron honey, made by bees from rhododendron pollen, contains a toxic substance called grayanotoxin. Depending on the dose, the poisonous honey can result in serious effects such as cardiac arrhythmia, fibrillation, and myocardial infarction. The purpose of this study is to investigate the effects of the poisonous RH of the Black Sea Region on the liver. Male mice were divided into five groups of twelve mice each, two being the control groups (distilled water) and the others being the rhododendron honey (RH) groups (25, 50, and 75 mg/kg) and 0.01 mg/kg grayanotoxin (GTx) groups. Liver tissues were collected 24 and 48 h later. The sections were stained with hematoxylin, eosin and PAS, then the histopathological score was performed. Significant statistical differences were observed between the RH and control groups in terms of congestion, steatosis, sinusoid dilatation, and inflammation. The control group demonstrated a normal liver structure in the light microscopy, while the GTx-applied 24 h group exhibited expansions in the sinusoids and congestion. Higher levels of congestion, steatosis, and inflammatory cells were seen in the GTx-applied 48 h group. In the same group, giant cells consisting of many nuclei were observed in the sinusoids. The results of the 25 mg RH-applied groups were similar in 24 and 48 h, histopathological score levels were increased slightly, congestion and steatosis were prominent in the 48 h group. Dense steatosis was seen in the hepatocytes around the vena centralis in 50 mg/kg RH-applied 48 h group. Congestion, steatosis and an increase in inflammatory cells were observed in the hepatocytes in the 75 mg/kg RH-applied 24-and 48 h groups. PAS (+) stained hepatocytes were decreased in the RH-and GTx-applied groups. The toxic effects of the rhododendron honey were observed in the mice liver tissue with respect to dose and time. KÜKNER, A.; ILTER, G.; SÖYLER, G.; RASGELE, P. G.; KEKEÇOGLU, M & KAMBUR, M. The effect of rhododendron honey on mice liver tissue. Int. J. Morphol., 34(3):842-847, 2016.

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“…F98 cells were seeded in 96-well plates (3 × 10 5 cell /mL). After culturing overnight, cells were incubated with the test compounds at various concentrations of rhododendron's lyophilized extract (6,12,25,50, 100, 500, 1000 and 2000 µg/mL in DMEM-F12), the medium only and 0.1% Triton X-100 served as negative and positive controls for 24 hours. Each concentration was tested in triplicate.…”

Section: Cell Viability and Cytotoxicity Assaysmentioning

confidence: 99%

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2018

IJPSR

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The genus Rhododendron has been widely used in traditional medicine. Rhododendron ponticum L. (R. ponticum) contains grayanotoxins with diterpene qualities. The aim of this study was to investigate in vitro cytotoxic and antiproliferative effects of R. ponticum L. extract on glioma in a rat glioma cell line (F98) and to calculate the amounts of grayanotoxins I and III. Rhododendrons were gathered from the Eastern Black Sea region of Turkey during the common flowering period. Their flowers were dried under suitable conditions, extracted with distilled water and lyophilized. The amounts of grayanotoxin I and III in the common rhododendron extract were determined by the chromatographic method. The cytotoxic and antiproliferative activities of different concentrations of the R. ponticum L. extract in mitochondrial (MTT) and lysosomal (Neutral red) assays was evaluated in glioma F98 cell lines. It was determined that the R. ponticum L. extract with a grayanotoxin I amount of 55.75 μg/kg and a grayanotoxin III amount of 7.4 μg/kg had a dose-dependent cytotoxic effect. IC 50 was found to be 122.8 µg/ml in the MTT and 79.61µg/ml in the neutral red (NR) assay. Previous studies show that R. ponticum L. may prevent high proliferative activity of tumor cells by its cytotoxic effects. The predictable amounts of grayanotoxin I and/or III may be preferred as a natural remedy for treatment of incurable glioma, which is one of the most common brain tumors in humans.

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Determination of Grayanotoxins in Honey by Liquid Chromatography Tandem Mass Spectrometry Using Dilute-and-Shoot Sample Preparation Approach

Cited by 20 publications

References 15 publications

Ormangülü Balı’nın Fare Kalp Kas Dokusu Lipitleri Üzerindeki Toksik Etkilerinin Moleküler Düzeyde İncelenmesi

Ormangülü Balı’nın Fare Kalp Kas Dokusu Lipitleri Üzerindeki Toksik Etkilerinin Moleküler Düzeyde İncelenmesi

The Effect of Rhododendron Honey on Mice Liver Tissue

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