Determination of grandiflorenic acid in organic and aqueous extracts of Montanoa tomentosa (zoapatle) by reversed-phase high-performance liquid chromatography

Antispasmodic and Antimicrobial Diterpenic Acids from Viguiera hypargyrea Roots

“…The active n-hexane extract was fractionated by vacuum liquid chromatography [6] over silica gel (300 g) using n-hexane-ethyl acetate mixtures to yield an active fraction (F-1, 15.0 g) eluted with n-hexane-ethyl acetate (97: 3 v/v, 6900 ml) which was composed of a diterpenic acid mixture. This mixture (500 mg) was further purified by column chromatography on silica gel (30.0 g) impregnated with 50 % silver nitrate solution [7], using mixtures of n-hexane-CH 2 Cl 2 resulting in the isolation of 40 mg of 1 (4 : Letter parison of mass, 1 H-, 13 C-, and DEPT-NMR data with literature values [8], [9]. Copies of the original spectra are available from the author of correspondence.…”

Increase of the Ejaculatory Potency by the Systemic Administration of Aqueous Crude Extracts of Cihuapatli (Montanoa Genus) Plants in Spinal Male Rats

“…It has been demonstrated that kaurenes that include the kaurenoic, grandiflorenic, kauradienoic, and monoginoic acids are the main biologically active compounds contained in the extracts of Montanoa tomentosa, Montanoa frutescens, and Montanoa grandiflora. [28][29][30][31] At present, the quantities of kaurenes contained in Montanoa tomentosa, Montanoa frutescens, and Montanoa grandiflora has been not elucidated, but might be speculated that equivalent concentrations of this acids could be contained in all Montanoas and that these kaurenes are responsible of the pro-ejaculatory effects of these plant extracts. Further experiments using individual fractions of Montanoas should be conducted to test this possibility.…”

Total assignment of 1H and 13C spectra of kauradien-9(11),16-oic acid with the aid of heteronuclear correlated 2D spectra optimized for geminal and vicinal 13C–1H coupling constants: or what to do when "INADEQUATE" is impossible

“…Full details of the isolation of 1 have been presented elsewhere (17). 2D experiments were performed on a Varian XL-200 spectrometer at a probe temperature of ca.…”