Determination of Glutathione

Jw, Patterson; Lazarow, Arnold

doi:10.1002/9780470110188.ch9

Cited by 129 publications

(40 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Tissue protein was estimated by the method of Lowry et al (15) . Glutathione content (reduced glutathione; GSH) was determined by the method of Patterson & Lazarow (16) . Superoxide dismutase (SOD) was assayed by the method of Kakkar et al (17) .…”

Section: Biochemical Analysismentioning

confidence: 99%

Amelioration of alcohol-induced hepatotoxicity by the administration of ethanolic extract ofSida cordifoliaLinn.

2012

View full text Add to dashboard Cite

Sida cordifoliaLinn. (Malvaceae) is a plant used in folk medicine for the treatment of the inflammation of oral mucosa, asthmatic bronchitis, nasal congestion and rheumatism. We studied the hepatoprotective activity of 50 % ethanolic extract ofS. cordifoliaLinn. against alcohol intoxication. The duration of the experiment was 90 d. The substantially elevated levels of toxicity markers such as alanine aminotransferase, aspartate aminotransferase and γ-glutamyl transferase due to the alcohol treatment were significantly lowered in the extract-treated groups. The activity of antioxidant enzymes and glutathione content, which was lowered due to alcohol toxicity, was increased to a near-normal level in the co-administered group. Lipid peroxidation products, protein carbonyls, total collagen and hydroxyproline, which were increased in the alcohol-treated group, were reduced in the co-administered group. The mRNA levels of cytochrome P450 2E1, NF-κB, TNF-α and transforming growth factor-β1 were found to be increased in the alcohol-treated rats, and their expressions were found to be decreased in the co-administered group. These observations were reinforced by histopathological analysis. Thus, the present study clearly indicates that 50 % ethanolic extract of the roots ofS. cordifoliaLinn. has a potent hepatoprotective action against alcohol-induced toxicity, which was mediated by lowering oxidative stress and by down-regulating the transcription factors.

show abstract

Section: Biochemical Analysismentioning

confidence: 99%

Amelioration of alcohol-induced hepatotoxicity by the administration of ethanolic extract ofSida cordifoliaLinn.

2012

View full text Add to dashboard Cite

show abstract

“…The method described by Patterson and Lazarow (1955) was employed. The principle is based on the fact that glutathione reacts with an excess of alloxan to produce a substance with an absorption peak at 350 nm.…”

Section: Determination Of Reduced Glutathione In the Brain Of Kindledmentioning

confidence: 99%

Antioxidant and antikindling effect of Tapinanthus globiferus growing on Ficus glumosa in pentylenetetrazole induced kindled rats

Abubakar¹,

Yunus²,

Abubakar³

et al. 2018

Afr. J. Biotechnol.

View full text Add to dashboard Cite

Tapinanthus globiferus growing on Ficus glumosa is a plant used locally for the treatment of epilepsy.It is found in West Africa on many tree crops. The present study aims to investigate the anti-kindling and antioxidant activity of the aqueous extract of T. globiferus. A total of 40 rats were divided into 4 groups (n=10). Groups 1 to 3 received 100, 200 and 400 mg/kg, respectively, of the extract orally, followed by 35 mg/kg of pentylenetetrazole (PTZ) i.p after an hour. Group 4 (control) was given 35 mg/kg of PTZ and normal saline and also observed for 30 min. This was repeated after every 48 h until all rats in the control group became fully kindled, that is, attained a racine score of 4 or 5 on three consecutive occasions. At the end of the experiment, the brain tissues of all rats were removed, homogenized and analyzed for antioxidant effect using lipid peroxidation, reduced glutathione, catalase, superoxide dismutase and uric acid tests. The extract was observed to significantly (p < 0.001) reduce the development of stage 5 kindling state as compared to the control group. The extract also significantly (p < 0.05) increased the activity of superoxide dismutase in the group treated with 400 mg/kg and also increased the activity of catalase in the 100 mg/kg treated group as compared to the control. The data obtained from this study suggests that the aqueous extract of T. globiferus growing on F. glumosa may possess bioactive compounds with antikindling and antioxidant effect and this may support its traditional use in the management of epilepsy.

show abstract

“…The tissue homogenates were prepared in appropriate buffers for each estimation. The concentration of the lipid peroxidation product malondialdehyde 2 (MDA), the non-enzymatic antioxidant reduced glutathione 3 (GSH), and activities of the enzymes catalase 4 (CAT), superoxide (GPx), glutathione-S-transferase 6 (GST) were determined spectrophotometrically using standard procedures. Analysis of important physico-chemical characteristics of aquarium water collected initially (before passing H 2 S) and during the experimental period was carried out according to standard procedures described in APHA 8 .…”

Section: Healthy Specimens Of Oreochromis Mossambicus (Tilapia)mentioning

confidence: 99%

Hydrogen Sulphide Exposure on Lipid Peroxidation and Antioxidant Enzymes in Fishes

2016

IJSR

View full text Add to dashboard Cite

Oxidative stress may ensue when the ability to buffer against reactive oxygen species (ROS) is exceeded either by excessive production of ROS or by depletion of antioxidant. This can alter cellular redox-poise and initiate a variety of responses via intracellular pathways. Hydrogen sulphide is a toxic gas which is harmful to aquatic organisms especially in the unionized form. In the present study two different concentrations of hydrogen sulphide is used for the toxicity assay in two test groups of Oreochromis mossambicus in time intervals of 12hrs, 24 hrs, 48hrs, 72 hrs and 96 hrs. With respect to the H 2 S concentration and duration of exposure, alterations were recorded in the concentration of lipid peroxidation products and antioxidants in the various organs of the test fishes. Malondialdehyde content increases in liver, gill, kidney and brain on exposure to hydrogen sulphide up to 48hrs and then the MDA content showed steady value up to 98hrs experimental period. Brain and kidney showed the maximum increase in concentration of reduced glutathione in initial stages (48 hrs) of hydrogen sulphide treatment. The gradual decrease in concentration of GSH in the tissues of H 2 S exposed fishes after 48 hrs up to 96hrs compared to the control shows the loss of adaptive mechanisms and the oxidation of GSH to GSSG. Slight increase in the activity of glutathione-S-transferase and decrease in activity of glutathione peroxidase demonstrated the incapability of the vital organs in neutralizing the peroxides generated in the oxidative stress condition.

show abstract

Determination of Glutathione

Cited by 129 publications

References 47 publications

Amelioration of alcohol-induced hepatotoxicity by the administration of ethanolic extract ofSida cordifoliaLinn.

Amelioration of alcohol-induced hepatotoxicity by the administration of ethanolic extract ofSida cordifoliaLinn.

Antioxidant and antikindling effect of Tapinanthus globiferus growing on Ficus glumosa in pentylenetetrazole induced kindled rats

Hydrogen Sulphide Exposure on Lipid Peroxidation and Antioxidant Enzymes in Fishes

Contact Info

Product

Resources

About