Determination of Glucose in Presence of Maltose. Rapid and Convenient Semimicromethod

Phillips, Louise Lang; Caldwell, M

doi:10.1021/ac60056a037

Cited by 4 publications

(3 citation statements)

References 10 publications

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“…In the presence of celobiose, glucose was determined by using the Phillips & Caldwell (1951) reagent. Deproteinization, when necessary, was effected with a zinc sulphatebarium hydroxide reagent (Somogyi, 1945).…”

Section: Methodsmentioning

confidence: 99%

Studies on carbohydrate-metabolizing enzymes. 10. Barley β-glucosidases

Anderson¹,

Cunningham²,

Manners³

1964

Biochemical Journal

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2. The carbon dioxide ratio, i.e. the ratio of the specific activities of expired carbon dioxide during the infusion of [1-14C]acetate and [2-14C]acetate, respectively, was 1-58 ± 0-17 (3) for non-pregnant sheep, 1-66 ± 0-25 (4) for sheep in early pregnancy, 1-74 + 0-14 (4) for sheep in late pregnancy, and 1-18 ± 0-11 (3) for under-fed sheep in late pregnancy. Calculations based on the last two ratios indicate that citric acid-cycle turnover in under-fed pregnant sheep is about one-quarter of the turnover found in well-fed pregnant sheep.3. The output of carbon dioxide is similar in all four groups of animals, but the percentage of carbon dioxide derived from acetate is low in the poorly-fed sheep in late pregnancy. The amount of acetate converted into carbon dioxide is lower in these animals but, because total aceta.te utilization is low in this group, the acetate presented to the tissues is oxidized as readily as in the sheep of other groups.

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Section: Methodsmentioning

confidence: 99%

Studies on carbohydrate-metabolizing enzymes. 10. Barley β-glucosidases

Anderson¹,

Cunningham²,

Manners³

1964

Biochemical Journal

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“…On incubation of fraction 2a with maltose, at 200 and pH 5 9 for 23 hr., no increase in reducing power was observed [with either Somogyi (1952) or Phillips & Caldwell (1951) reagents] and glucose was not detected by paper chromatography. Under similar conditions, the P-amylolysis limit of glycogen and amylopectin fl-dextrin increased by 25 and 23 % respectively.…”

Section: Preparation Of Isoamyla8ementioning

confidence: 99%

Studies on carbohydrate-metabolizing enzymes. 7. Yeast isoamylase

Gunja

Manners

Maung

1961

Biochemical Journal

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“…20 The reducing values of hydrolyzates of the Schardinger dextrins were determined by the copper method of Somogyi.21 The unit concentration of the amylase refers to the concentration required to cause the formation of maltose equivalents corresponding to 20% theoretical maltose in 30 minutes at 40°from 1% Lintner soluble potato starch adjusted to 0.01 M acetate and pH 5.0.7 Glucose and maltose were determined in the hydrolyzates by selective fermentation22-24 and by chromatographic techniques.25 -27 Glucose was determined also by a copper acetate method. 28 Results and Discussion Hydrolysis of Linear Substrate, Reducing Values. The data given in Table I are typical of the results obtained when highly purified4 or crystalline6 maltase-free taka amylase reacted with the linear fraction from corn starch.8 Increasing concentrations of taka amylase increased the rate and the extent of the hydrolysis of the substrate until a limit was reached, at the equivalent of 109% of the theoretical maltose.…”

Section: Introductionmentioning

confidence: 99%