Determination of glucose in blood by flow injection analysis and an immobilized glucose oxidase column

A new long-wavelength fluorigenic substrate for alkaline phosphatase: synthesis and characterisation

“…100 ml. 12 Cyclodextrins and (3-[3-cholamidopropyl]-dimethylamino)-1-propane sulfonate (CHAPS) were also products from Sigma. Fluorescence detection in flow injection analysis experiments was performed using a small laboratory constructed spectrometer incorporating a 635 nm diode laser light source (Power Technology Inc.), a 640 nm cut-off filter in the emission beam, and a silicon photodiode.…”

Micro Flow Injection Analysis Combined with a Separation Technique for the Urinary Glucose Assay

“…There was no significant effect of the pH value from 4 to 10, although the optimum pH value of 4.5 was reported in the case of voltammetry using GOD. 18 The present method, therefore, has an advantage that a pH treatment of the sample is unnecessary, because the normal pH range of human urine is from 5.0 to 7.4. The proposed µFIA system was then applied to multicomponent samples.…”

Kinetic Flow-injection Determination of Hydrogen Peroxide by Use of Iron(III)-catalyzed Coloration and Its Application to the Determination of Biological Substances

“…[24][25][26] After being cleaned with 5% nitric acid and water, and dried at 95˚C, the CPG was then silanized with 5 g of 10% (3-aminopropyl)triethylsilane in 45 ml of acetate buffer solution (pH 3.75) at 75˚C for 2.5 h. The silanized CPG was rinsed with water and dried at 95˚C to allow storage. A portion (5 g) of 2.5% glutaraldehyde was added to 1 g of the silanized CPG.…”

“…Especially, FI procedures including immobilized enzyme reactors, which lead to the generation of hydrogen peroxide, have provided attractive methods for specific determinations of clinically interesting substances, such as glucose, cholesterol and glutamic acid. 5,16,[19][20][21][22][23][24][25][26] Although these methods could determine micro-or submicromolar levels of hydrogen peroxide or biological substances, they suffer from disadvantages due to the use of unstable and/or expensive reagents and of complicated equipment.…”