Determination of Glimepiride in Human Plasma by LC–MS–MS

Yüzüak, N.; Özden, Tuncel; Eren, Şule; Ozilhan, Selma

doi:10.1365/s10337-007-0368-6

Cited by 17 publications

(7 citation statements)

References 8 publications

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“…8 There are two polymorphs of this molecule reported in the literature, form I and form II, of which form II has about 3.5-fold higher solubility than that of form I. 9 There is a growing number of studies describing the determination of glimepiride concentration in biological fluids [10][11][12][13][14][15][16][17][18] and pharmaceutical formulations [19][20][21][22][23][24][25][26][27][28][29][30] using a variety of methods. However, there is no dissolution method for glimepiride tablets in the literature.…”

Section: Introductionmentioning

confidence: 99%

A Discriminating Dissolution Method for Glimepiride Polymorphs

Bonfílio

Pires

Ferreira

et al. 2012

Journal of Pharmaceutical Sciences

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Section: Introductionmentioning

confidence: 99%

A Discriminating Dissolution Method for Glimepiride Polymorphs

Bonfílio

Pires

Ferreira

et al. 2012

Journal of Pharmaceutical Sciences

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“…[11] Also, after oral administration, glimepiride is completely (100%) absorbed from the gastrointestinal tract. [12] A significant absorption of glimepiride within 1 h after administration and peak drug levels ( C max ) at 2–3 h was reported. [13] In addition, recently, it was found that licorice juice significantly ( P < 0.05) increased the AUC 0–6 , C max , and t 1/2 of glimepiride and significantly ( P < 0.05) decreased the clearance and elimination rate constant, whereas grapefruit juice found to have no significant ( P > 0.05) effect on glimepiride pharmacokinetics.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of liquid chromatography method for determination of glimepiride in presence of (Vimto®) soft drinks in rats: application to pharmacokinetics studies

Hamad¹,

Rahhal²,

Dayyih³

et al. 2019

J Pharm Bioall Sci

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Context: Diet and beverages are thought to have notable effects on drugs. Recently, this relationship has received significant consideration. Aims: To develop and validate a simple, rapid, and sensitive method for the determination of glimepiride in rat serum. This will be performed using high-performance liquid chromatography–mass spectrometry (HPLC-MS/MS). Potential pharmacokinetic interactions between glimepiride and the soft drink, Vimto, will also be investigated in the serum of experimental rats. Materials and Methods: HPLC-MS/MS was constructed and clarithromycin was used as an internal standard. Results: The method was validated in terms of linearity, precision, accuracy, stability, and system suitability parameters. The method was found to be satisfactory and suitable for the determination of glimepiride. The precision of glimepiride was high (coefficient of variation, CV% <15%), the accuracy over all 3 days of validation was within the accepted criteria. Glimepiride peak serum concentration ( C max ) was 126.01 ng/mL and was reached within 1 h ( T max ) of administration. Mean area under curve (AUC) was 964.70 ng/mL and was reached within 24 h of administration. The Vimto soft drink significantly ( P < 0.050) reduced glimepiride peak serum concentration to 57.87 ng/mL and was reached within 2 h of administration. AUC was significantly reduced to 335.04 ng * h/mL ( P < 0.050). Conclusion: Glimepiride pharmacokinetic parameters such as C max and AUC were significantly affected by the Vimto soft drink. Therefore, this study developed a simple, rapid, and sensitive method for validation and determination of the effects of soft drinks on drugs using the LC-MS/MS method.

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“…Atorvastatin (ATV)-Glimepiride (GLI) combines a competitive inhibitor of HMGCoA reductase and a sulfonylurea anti-diabetic drug GLI. In the references few bioanalytical methods were reported for the determination of ATV and GLI separately and in combination with another products [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21]. Two methods have been reported for the simultaneous determination of ATV and GLI [22,23].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Atorvastatin and Glimepiride by LC-MS/MS in Human Plasma and Its Application to a Pharmacokinetic Study

Kumar¹,

Reddy²,

Thriveni³

et al. 2012

AJAC

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The aim of the proposed research work was to develop and validate a simple, selective, high sensitive and high-throughput assay for the simultaneous estimation of Atorvastatin and Glimepiride in human plasma using liquid chromatography tandem mass spectrometry (LC-MS/MS). Atorvastatin-Glimepiride combines a competitive inhibitor of HMG-CoA reductase and a sulfonylurea anti-diabetic drug. The purpose of this study was to develop single method for Atorvastatin and Glimepiride in plasma by liquid chromatography-tandem mass spectrometry (LC-MS/MS) that would result into a simultaneous estimation of Atorvastatin and Glimepiride avoiding acid-lactone inter conversions right from sample collections to analysis on the LC-MS/MS. Sample collection procedure optimized for Atorvastatin holds good for Glimepiride, hence resulting into a simultaneous estimation of Atorvastatin and Glimepiride. Liquid-liquid extraction and liquid chromatography coupled to positive ion mode tandem mass spectrometry was used to develop the method and was validated according to US FDA guidelines. The calibration curves for two analytes were linear (R2 ≥ 0.9950, n = 4) over the concentration range of 0.2 -30 ng/mL for Atorvastatin and 1 -250 ng/mL for Glimepiride. Mean extraction recoveries 80.34 ± 9.43 for Atorvastatin and 88.19 ± 7.13 for Glimepiride. Intra-and inter-run mean percent accuracy was between 85% -115% and percent imprecision was ≤15%. Stability studies revealed that Atorvastatin and Glimepiride were stable in plasma during bench top (10.5 h at room temperature), in Injector (47.5 h), at the end of three successive freeze and thaw cycles and long term at −65˚C ± 15˚C for 114 days. The method was successfully applied to the study of pharmacokinetics of Atorvastatin and Glimepiride in healthy volunteers. Simultaneous estimation of Atorvastatin and Glimepiride is cost effective, reduces analysis cycle time, enables effective utilization of resources and reduces bleeding burden on human volunteers.

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Determination of Glimepiride in Human Plasma by LC–MS–MS

Cited by 17 publications

References 8 publications

A Discriminating Dissolution Method for Glimepiride Polymorphs

A Discriminating Dissolution Method for Glimepiride Polymorphs

Development and validation of liquid chromatography method for determination of glimepiride in presence of (Vimto®) soft drinks in rats: application to pharmacokinetics studies

Simultaneous Determination of Atorvastatin and Glimepiride by LC-MS/MS in Human Plasma and Its Application to a Pharmacokinetic Study

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