Determination of glabridin in human plasma by solid-phase extraction and LC–MS/MS

Aoki, Fumiki; Nakagawa, Kaku; Tanaka, Akiyoshi; Matsuzaki, Kazue; Arai, Naoki; Mae, Tadahiko

doi:10.1016/j.jchromb.2005.09.012

Cited by 51 publications

(31 citation statements)

References 17 publications

(17 reference statements)

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“…Measurements of waist and hip, body weight (and calculated BMI), and blood pressure/pulse rate, plus hematology, urinalysis, and medical history review were conducted at each visit. Plasma glabridin concentrations were measured at weeks 2 and 4 of ingestion and post-ingestion week 2, in accordance with the method described by Aoki et al 17) Briefly, approximately 10 ml of venous blood was collected in each tube containing heparin. After collection, the blood samples were centrifuged (himac CF7D2; HITACHI, Tokyo, JAPAN) for 10 minutes, and plasma samples were frozen until glabridin concentration measurement using solid-phase extraction and LC-MS/MS [an SIL-HTC or an LC-10A HPLC system (Shimadzu Corporation, Kyoto, Japan) coupled to an API 4000 mass spectrometer (Applied Biosystems/MDS Sciex, Foster City, CA)].…”

Section: Study Design ---mentioning

confidence: 99%

Licorice Flavonoid Oil Effects Body Weight Loss by Reduction of Body Fat Mass in Overweight Subjects

Tominaga

Mae

Kitano

et al. 2006

JOURNAL OF HEALTH SCIENCE

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Licorice flavonoid oil (LFO) is a new dietary ingredient for functional foods consisting of licorice hydrophobic polyphenols in medium-chain triglycerides (MCT). In an effective dose finding study conducted previously, LFO has exhibited a dose-dependent body fat-reducing effect. Here we report the weight-reducing effect of LFO in a placebo-controlled, double-blind, long-term (12 weeks) ingestion study at 300 mg/day, the minimal effective dose observed in the dose finding study. A total of 103 overweight subjects [body mass index (BMI): 24-30] completed this study and were analyzed. Body weight increased in the placebo group, but was maintained at close to preingestion level in the LFO group, resulting in significant (p < 0.05) differences in the changes in body weight and BMI between the LFO group and the placebo group at each time-point. Dual-energy X-ray absorptiometry (DXA) measurement of body fat indicated that the weight-reducing effect was attributable to reduced body fat. No clinically significant adverse events occurred during the 12-week ingestion period. To confirm the safety of LFO for practical use we also conducted a placebo-controlled, double-blind safety study in 40 overweight subjects with a 4-week excessive ingestion at 1800 mg/day; 6 times the dose of the 300 mg/day study that exhibited a weight-reducing effect. No clinically significant adverse events occurred during the 4-week ingestion period. Based on these findings in both human studies it was shown that LFO is a safe ingredient for functional foods even for long-term or excessive ingestion, with a potential weight-reducing effect.

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Section: Study Design ---mentioning

confidence: 99%

Licorice Flavonoid Oil Effects Body Weight Loss by Reduction of Body Fat Mass in Overweight Subjects

Tominaga

Mae

Kitano

et al. 2006

JOURNAL OF HEALTH SCIENCE

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“…One such source is Glycyyrhiza glabra (L.) commonly called Mulhati or Liquorice, the root of the this plant species, has been used medicinally for more than 4000 years (Aoki et al, 2005). The genus glycyrrhiza consists of approximately 30 species, in which six species produce a sweet saponin glycyrrhizic acid (GA), and they are widely used in Asia countries.…”

Section: Introductionmentioning

confidence: 99%

Studies on the development and storage of cocoa -mulhati guava based products

Pawar

Sharma

Garg

et al. 2017

AJDFR

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Cocoa-mulhati guava toffee/ bar/nuggets are concentrated product having good nutritive value, appeal and are consumed readily as a confectionery product. The varying amount of ingredients such as sugar, skim milk powder and mulhati were added to guava pulp for formulating cocoa-mulhati guava product. The prepared products were subjected to physicochemical (total soluble solids, ascorbic acid, acidity, sugars and total phenols) and nutritional analysis. The value added products (toffee/ bar/ nuggets) were also evaluated for colour, appearance, flavor, taste, texture and overall acceptability. Cocoa-mulhati-guava based product (toffee/bar/nuggets) prepared with 1 kg guava pulp, 600 g sugar, 150 g skim milk powder and 40 g of mulhati powder was found to be best in terms of sensory acceptance. It was found to have moisture, fat, protein and carbohydrate of 7.8 percent, 16 percent, 0.1 percent and 76 percent respectively. Total soluble solids, total and reducing sugars increased significantly with increase in storage time. Value added products were found to be acceptable even after three months of storage period; however, there was decrease in ascorbic acid, total phenols and overall acceptability of products during storage period.

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“…Cellular extracts were prepared from whole inserts by shaking slowly for 30 min with 100 L methanol for the apical side and 700 L for the basolateral side. The recovered solutions were submitted to solid-phase extraction by methanol with Sep-PakR (Vac 1 cc C8, Waters), and analyzed by HPLC ( 19 ).…”

Section: Chemicalsmentioning

confidence: 99%

“…1), and may also escape conjugation. We have previously found that dietary glabridin appeared in human plasma in its free form ( 19 ). The reported obvious bio-functions may correlate with the high bioavailability of glabridin.…”

mentioning

confidence: 94%

Absorption of Dietary Licorice Isoflavan Glabridin to Blood Circulation in Rats

Ito

Hashimoto

et al. 2007

J Nutr Sci Vitaminol

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Summary Bioavailability of glabridin was elucidated to show that this compound is one of the active components in the traditional medicine licorice. Using a model of intestinal absorption, Caco-2 cell monolayer, incorporation of glabridin was examined. Glabridin was easily incorporated into the cells and released to the basolateral side at a permeability coefficient of 1.70 Ϯ 0.16 cm/s ϫ 10 5 . The released glabridin was the aglycone form and not a conjugated form. Then, 10 mg (30 mol)/kg body weight of standard chemical glabridin and licorice flavonoid oil (LFO) containing 10 mg/kg body weight of glabridin were administered orally to rats, and the blood concentrations of glabridin was determined. Glabridin showed a maximum concentration 1 h after the dose, of 87 nmol/L for standard glabridin and 145 nmol/L for LFO glabridin, and decreased gradually over 24 h after the dose. The level of incorporation into the liver was about 0.43% of the dosed amount 2 h after the dose. These detected glabridins were in the aglycone form and not conjugated forms. The bioavailability was calculated to be AUC inf of 0.825 and 1.30 M ·h and elimination T 1/2 of 8.2 and 8.5 h for standard glabridin and LFO, respectively. Adipocytokine levels were determined in the rats. The secreted amount of monocyte chemoattractant protein-1 was significantly lower in the glabridin group compared to control vehicle group. Thus, dietary glabridin was at least partly incorporated into the body in an unchanged form, though most dietary flavonoids are converted to non-active conjugate forms during intestinal absorption.

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Determination of glabridin in human plasma by solid-phase extraction and LC–MS/MS

Cited by 51 publications

References 17 publications

Licorice Flavonoid Oil Effects Body Weight Loss by Reduction of Body Fat Mass in Overweight Subjects

Licorice Flavonoid Oil Effects Body Weight Loss by Reduction of Body Fat Mass in Overweight Subjects

Studies on the development and storage of cocoa -mulhati guava based products

Absorption of Dietary Licorice Isoflavan Glabridin to Blood Circulation in Rats

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